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Evolution of Recombinant Lymphocytic Choriomeningitis Virus/Lassa Virus In Vivo Highlights the Importance of the GPC Cytosolic Tail in Viral Fitness

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Academic year: 2019

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Figure

FIG 1 A mutant evolves in an rLCMV/LASV wt GP-infected HHD mouse. (A) HHD mice (rLCMV/LASV wt GP
FIG 2 Mutation in the GP-2 cytosolic tail increases fitness of virus. (A) TherLCMV/LASV mut GP
FIG 3 In vivoof the predicted cytosolic location of the observed K461G mutation. (B) LCMV Cl13, LASV Josiah, and LASV mut GP cytosolic tail sequences were aligned, andresidue conservation is indicated
FIG 4 growth kinetics of mutant virus. (A) MC57, BHK-21, and Vero cells, each in triplicate, were infected at 60 to 70% confluence with eitheran MOI of 0.01 with rLCMV/LASV GP, the mutated rLCMV/LASV mut GP, or combinations between VGKS, KGGS, and VGGS with
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