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Configuration of Viral Ribonucleoprotein Complexes within the Influenza A Virion

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FIG 1 Isolation and purification of RNPs. (A) Gel electrophoresis of the proteins of each fraction after ultracentrifugation through 30% to 70% glycerolgradients
FIG 2 Negative-staining immuno-EM of purified RNPs with an anti-NP (A),anti-PA (B), anti-PB1 (C), or anti-PB2 (D) antibody or anti-FLAG antibody(E) conjugated with 5-nm gold particles
FIG 4 Immunostaining of thin-sectioned virions. The sections were sequentially incubated with an anti-NP (A), anti-PA (B), anti-PB1 (C), anti-PB2 (D), oranti-FLAG (E) monoclonal antibody or without a primary antibody (F) and with a secondary antibody conju
FIG 6 Schematic diagrams of RNPs within a virion. (A) The diagram showing aRNPs are vertically packaged in a virion

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