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MicroRNA-Based Attenuation of Influenza Virus across Susceptible Hosts

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Figure

FIG 2 miR-21 is abundantly expressed in IAV-susceptible hosts. Small RNA Northern blot analysis wasperformed to analyze expression of miR-21, miR-29, and U6 in MDCK, DF-1, A549, and MLE15 cells (A),A549 and MLE cells infected with PR8 at an MOI of 1 (B), and lungs of a naive mouse and a mouseinfected with 40 PFU of PR8 (C).
FIG 3 Generation of miR-21 knockout MDCK cells. (A) Model demonstrating use of dual-guide RNAs toeliminate the entire primary miR-21 locus
FIG 4 miR-21-targeted virus is attenuated in influenza-susceptible hosts. (A) miR-21 KO MDCK and A549 cells were infectedat an MOI of 0.5 with NP-21t or NP-ctrl virus for a single cycle of replication
FIG 5 Vaccination with NP-21t virus confers protection during secondary IAV infection

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