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Cryo-electron Microscopy Structures of Expanded Poliovirus with VHHs Sample the Conformational Repertoire of the Expanded State

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Figure

FIG 1 Amino acid sequences and cryo-EM reconstructions of four anti-C/anti-H-VHH complexes with expanded poliovirus.(Top) Sequence alignment of the four VHHs shows that they are very similar to one another
FIG 2 Quality of the electron density in the reconstructions of anti-C-VHH complexes with poliovirus.(Left) Slice through the VP2 beta barrel illustrating an area where rigid-body fitting of the native atomicmodel accounts satisfactorily for the PVSP17B exp
TABLE 1 Amino acids that form structurally consistent core regionsa
FIG 3 Anti-C VHH PVSP17B can bind to purified 80S and 135S poliovirions but not to 160S native virions.Poliovirions, in various states of expansion, were incubated at 25°C, first with the VHH in solution andthen with Ni-NTA-agarose beads that bind to the His
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