Commercially available Technology Test Data from Lab and Field Studies

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(2)

Packaging Solution using IGR Methoprene for Insect Control

• Commercially available Technology

• Test Data from Lab and Field Studies

• Objective: Reduction in use of fumigants and

(3)

IGR

(Insect Growth Regulator)

X

Methoprene is used extensively in Integrated Pest Management Programs

Adult

Pupa

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Feed Products

Purina Brand markets a “Feed-through” for Beef Cattle and Horses to control fly populations in feedlots

Grain Storage

Diacon(Central Garden and Pet)- Markets a powdered additive to grain bins during harvest and transfer for insect control

Pet Flea and Tick Treatments

Applied to pets to control fleas and ticks in Neck applications and collars

Food Manufacturing Facility

Fogging solutions in warehouses and plants to control insect populations

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Commercially Available Products

Film/Woven Packaging Solution

PGT IGR 30000 Film Integration Additive

•Liquid additive to film making process • Combines and resides in films

• Manually combines with Pellets • Can be metered directly in extruder • Fully Compliant w/ all regulatory agencies

• No Odor or Taste • Adds insect control

Paper Packaging Solution

PGT 23207 Water Based Coating

•_{Clear water based topcoat}

•_{Typically printed over graphic images} • Replaces existing coatings

•_{Duplicates existing coating in}

COF Gloss

Rub Resistance

•_{Fully Compliant w/ all regulatory}

agencies

•_{Adds insect control} Controls for over

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FDA Status

ProvisionGardTM_{complies with FDA requirements for Food, Feed & Tobacco}

packaging EPA Status

ProvisionGard™ is registered with the EPA (registration # 81390-1) CONEG (Coalition of Northeastern Governors)

ProvisionGard™ complies with the requirements of the toxics in packaging law(s)

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Test Results

All data generated using USDA

Protocols and trained Entomologists

.

(8)

Evaluation of ProvisionGard for Control of Flour

Beetles and Indian Meal Moth in Paper and

Woven Poly Bags

Go to

www.pvgard.com

to watch video

Jade Vardeman

Research Entomologist

Moses Biologic, LLC

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Treated vs Untreated Cartons with Water Based Coating

0 100 200 300 400 500 600 1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 Ins e c t C ount Shipper Number ProvisionGard Study Breakfast Cereal Cartons

September 2009

PG Treated Control

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Rice Packaged in Treated Cartons and Treated Corrugate Boxes

CBR Cartons (18 cartons/shipper) Control Treated Total # of shippers 14 10 Total # of insects 329 0 Average # insects/shipper 23.5 0.0

Range of insect count/shipper 0 – 132 0 – 0

Average reduction in

treated vs. control 100% efficacy

Brown Rice Cartons (18 cartons/shipper)

Control Treated

Total # of shippers 8 11

Total # of insects 60 5

Average # insects/shipper 7.5 0.5

Range of insect count/shipper 0 – 37 0 – 3

Average reduction in

treated vs. control 93.4% efficacy

CBR Poly (6 bags/shipper) In Corrugate Boxes

Control Treated Total # of Shippers 28 28 Total # of Insects 182 3 Average # of insects/shipper 6.5 0.1

Range of insect count/shipper 2 – 13 0 – 1

Average Reduction in Treated vs. Control 99% efficacy

Whole Grain 5# Poly (12 bags/shipper)in Corrugate Box

Control Treated Total # of Shippers 10 15 Total # of Insects 95 0 Average # of insects/shipper 9.5 0

Range of insect count/shipper

1– 23 0 – 0

Average Reduction in

Treated vs. Control

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“Blinded” Pet Food: Multiwall Bags

0 20 40 60 80 100 120 1 11 21 31 41 51 61 71 81 91 101 111 121 Treated In s ec t Count

Bag Number Control

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Evaluation of ProvisionGard for

Control of Flour Beetles

Packaging- Film Woven Poly

Dr. Frank Arthur

Research Entomologist

USDA-ARS-CGAHR

Manhattan, KS 66502

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Methods-Test 1

• Circular disks of packaging cut to fit bottom portion of a 9.6 in2 plastic Petri dish • Disks with inner and outer portions of the packaging

• Ten 4-week old red flour beetle larvae or ten confused flour beetle larvae of same age put in separate dishes with 300 mg flour

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Results to Date

• Confused flour beetle the more tolerant of the two species (consistent with other studies) • Outer portion still active after 8 months

• Inner portion losing efficacy

Inner Outer Month 0 5.0 ± 2.0 0.0 ± 0.0 Month 2 3.3 ± 2.1 1.6 ± 1.6 Month 4 23.3 ± 3.3 5.0 ± 3.4 Month 6? 66.7 ± 21.1 6.7 ± 3.3 Month 8 30.0 ± 12. 1 0.0 ± 0.0 • Conclusions

% Emergence-Red Flour Beetle

Inner Outer Month 0 41.7 ± 14.7 6.7 ± 6.7 Month 2 81.7 ± 9.0 5.0 ± 3.4 Month 4 90.0 ± 5.7 40.0 ± 8.1? Month 6 83.3 ± 4.2 1.7 ± 1.7 Month 8 93.3 ± 3.3 15.0 ± 6.1

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Field Applications Data

• Bartlett Milling reports a 90% reduction in returns the first Year.

• Mars Food reports a 100% elimination of returns due to insect damage in 2011 for corrugated bulk shipments. Normally 4-5 truckloads are returned annually.

• Southern States reports no incidents of insect damage in packaged seed products for 2011.

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ProvisionGard

TM

_{Test Results}

0 2 4 6 8 10 12 14 16 18 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 In sect s p er S h ip p er Shipper Number No Column represents Zero Insects

ProvisionGard

Pet Food Multiwall -

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90 Day Field Study- SOS Bag

Pet Food SOS Bags Fort Worth Texas Distribution Center

Control Treated

Total # of Shippers 41 41

Total # of Insects 58 3 Average # of insects/shipper 1.4 0.07

Range of insect count/shipper 2 – 16 0 – 1

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ProvisionGard

TM

_{Test Results}

Learnings from Testing Results



_{Infestation typically reduced 85-99%}



Works on both exterior & buried substrate



_{Effective on a broad spectrum of insects}

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