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Comparative examination of various PCR-based methods for DNMT3A and IDH1/2 mutations identification in acute myeloid leukemia

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Figure

Figure 1 Restriction analysis of DNMT3A R882H mutations. 1) Agarose gel analysis of restricted products of 5 positive (12, 34, 57, 65, 187)and 2 negative (54, 143) patients
Figure 2 Sensitivity analysis of DNMT3A R882H detection. 1) Endonuclease restriction analysis of serial dilutions of DNMT3A R882H; Undilutedmutation ratio was 59% (estimated by sequencing)
Figure 5 Melting curve profiles of wt allele and IDH2 140Q G>A. Vertical axis corresponds to changes in the fluorescence signal over time(dF/dT)
Figure 6 Sensitivity analysis of IDH2 R140Q detection. 1) Serial dilutions of IDH2 R140Q: Undiluted mutation ratio was 45% (estimated bysequencing)
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