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Cell culture-based analysis of postsynaptic membrane assembly in muscle cells

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Figure

Fig.  1:  Schematic  representation  of  the  secondary-plating method. C2C12 myoblasts were plated on uncoated plastic culture dishes in growth  medium, and after  two  days, the  medium  was replaced by Differentiation  Medium  to  induce  myoblast  fusi
Fig.  3:  Effect  of  substrate  laminin  on  Rac  activity  in  replated myotubes. Myoblasts were cultured and replated as described in Fig
Fig.  4:  Effects  of  blocking  Rho-mediated  signaling  on  AChR clustering  induced  by  substrate  laminin
Fig.  5:  Combinatorial  effects  of  agrin  and  laminin  in  replated myotubes. To study the combined effects of agrin and laminin, replated myotubes were used to analyze AChR aggregation patterns induced by soluble and immobilized agrin and laminin

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