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Panfungal PCR and Multiplex Liquid Hybridization for Detection of Fungi in Tissue Specimens

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Figure

TABLE 1. Primers and probes used in the fungal ITS PCR liquid hybridization protocol
TABLE 2. Summary of specimen data and results of culture, direct microscopy, ITS PCR-hybridization, and sequencing
FIG. 3. Hybridization sensitivity and efficiency. The indicated amount of single-stranded ITS PCR amplification product was hybridized in a mixture of three probes.The amount of hybridized product (peak area) was measured on the ALFexpress sequencer, and an average hybridization efficiency (%) was calculated for the four

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