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Hypoxia-inducible factor 1 mediates TAZ expression and nuclear localization to induce the breast cancer stem cell phenotype

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Figure

Figure 1: The WWTR1 gene encoding TAZ is a HIF-1 target gene. (A) Reverse transcription and quantitative real-time PCR (RT-qPCR) were performed to quantify TAZ mRNA levels in 5 breast cell lines following exposure to 20% or 1% O2 for 24 h
Figure 2: Analysis of TAZ target gene expression. (A) RT-qPCR was performed to quantify CTGF, PAI-1 and Survivin mRNA levels in breast cell lines following exposure to 20% or 1% O2 for 24 h
Figure 3: Analysis of TAZ subcellular localization. (A) MDA-MB-231 subclones (NTC, sh1α, and sh2α) were exposed to 20% or 1% O2 for 48 h and stained with anti-TAZ antibody (green), Alexa Fluor 568-conjugated phalloidin to detect cytosolic F-actin (red), an
Figure 4: Analysis of LATS2 expression. (A) Immunoblot assays were performed to analyze actin, LATS1, and LATS2 protein levels in 4 breast cancer cell lines following exposure to 20% (N) or 1% (H) O2 for 48 h
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