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Bmi1 regulates murine intestinal stem cell proliferation and self renewal downstream of Notch

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Figure

Fig. 1. Notch and βrequired for maintaining intestinalhomeostasisGOF mutantand Alcian Blue staining of goblet cells onintestinal crypts of the indicated genotypes.(D,E) Quantification of the number of goblet(D) and Ki67-positive cells (E) per crypt unit in
Fig. 2. Notch and βmousestatistical significance was determined using Studentantibodies
Fig. 3. Bmi1***animals. One representative from twoindependent experiments is shown.(G) ISH analysis of the indicated ISCgenes in(H) Quantification by using qRT-PCRanalysis of the mRNA levels in the cryptfractions
Fig. 4. Increased p16INK4a*Ctnnb1(the latter a control for the lack of proteinexpression) inembryonic day (E)15.5
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