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Highly efficient targeted mutagenesis in axolotl using Cas9 RNA guided nuclease

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Academic year: 2020

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Figure

Fig. 1. Efficient targeted mutagenesis of EGFPdisplayed no apparent EGFP expression contain indels at the targeted site (yellow)
Fig. 2. Efficient targeted mutagenesis ofbrachyury ortholog. (A,B) Whereas animalsinjected with nls-EGFP mRNA display nophenotype at 21 days post-fertilization (A), theirsiblings injected with sgRNA directed against aputative brachyury ortholog and cas9dis
Fig. 3. Efficient targeted mutagenesis at the bambi locus. (A) Sequencing of the targeted region of the bambi locus from three bambi RGN-injected5-day-old whole embryos reveals a high indel frequency at the targeted site

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