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Development of agrobacterium mediated transformation protocol for mature seed derived callus tissues of citrus cultivar ‘gailiangcheng orange x weizhang satsuma mandarin’

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Academic year: 2020

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Figure

Table 1. Media composition
Figure 1. Effect of Kanamycin concentrations on survival of callus lines. Each experiment was repeated three times
Figure 3.  Factors influencing gfp expression and transformation efficiency 3a) Effect of optical density of Agrobacterium culture (O.D600) on gfp expression and transformation efficiency 3b) Effect of immersion time on gfp expression and transformation ef
Figure 5. PCR analysis of the transgenic plants with primers specific to: 5a) gfp gene and 5b) nptII gene

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