Bertrand Guillotin. Bordeaux, FRANCE Tel:

Bertrand Guillotin

Place of birth : Lilles (59) Citizenship : French Married

email : bertrand.guillotin@inserm.fr

Education at the University of Victor Segalen, Bordeaux2:

2001 – 2004 : Ph.D thesis at the INSERM 1026 BioTis (ex U577), on the molecular interactions between endothelial cells and osteoprogenitors and the effects of these interactions on cell differentiation. Supported by a grant from Région Aquitaine/ Europe. Thesis defended on December 6th _2004.

2000 – 2001 : Post-graduate diploma in biology of health.

1999 – 2000 : Master’s Degree in Biology, concentration in Cellular Biology and Physiology. 1998 – 1999 : Bachelor’s Degree in Biology, concentration in Cellular Biology and Physiology. 1997 – 1998 : Two-year diploma in Science, concentration in Biology.

1996 – 1997 : First year of Medical School. Professional experience :

2005 - 2007: Starting on February 2005, post-doctoral research associate at Washington University School of Medicine (Missouri, USA), in Dr. Roberto Civitelli's lab. This team is focused on cell/cell interaction involved in bone development.

2007 - 2008 : From March 1st to August 31st, 6 months position as an assistant professor at Victor Segalen University of Bordeaux (UB2). Research project was aimed at unveiling genetic mechanisms involved in pluripotency of mouse embryonic stem cells. This project was coordinated by Dr. Hélène Bœuf, in lab UMR 5164 CNRS-CIRID directed by Pr. Jean François Moreau. I extended this collaboration as a research associate until the 31st of July 2008 (funded by European Fungen ES). Current position :

2008… Starting on September 2008, 2 year position as a research associate at INSERM unit 577 "Biomaterials and Tissue Repair" directed by Dr. Joëlle Amédée. Within the team "TEAL" coordinated by Dr. Fabien Guillemot, I develop the project entitled "Study and assembly of 3D biological structures engineered by Laser Assisted Bioprinting".

Skills:

- Bilingual in French and English

- Ability to work with mice (Washington University agreement),

- Ability to work with radioactivity (Washington University agreement),

- Cell culture : primary human osteoprogenitor cells, primary HUVEC, primary mouse calvaria cells, primary mouse bone marrow cells, cell lines (ROS, UMR, packaging cells, MG63, Eahy926, CGR8 mouse embryonic stem cell line)

- Piximus (bone mineral density measurement device on small animal)

- Molecular biology: RT-PCR, Real time quantitative PCR (Applied Biosystems, Biorad, Roche, Stratagene), cloning, in vitro transcription

- Biochemistry: cytochemistry of alkaline phosphatase, von Kossa /alizarin red/alcian blue/oil red, immunocytochemistry, western blot, cytotoxicity tests, EMSA, dye (Lucifer yellow) micro-injection into the cell (for gap junction study)

- Laser Assisted Bioprinting - Teaching

- Ability to work with deadlines and in a team.

Fond of sports, skilled skier, love surfing and kite surfing, play tennis and basket ball. Like to travel (North America, Central America, Africa, Europe)

Memberships and Certificates:

- Followed a one year training program by the firemen of Bordeaux during years 1998 and 1999, for beach surveillance (ocean and lakes of Gironde) during summers 98 and 99.

- First Aid Certificate

- French Life Guard Certificate (1998-2003) Teaching :

2007 Professor assistant at University of Bordeaux2 on Molecular and Cell Physiology, under the direction of Pr. Nadine Thézé.

2008 Teacher on Cell Biology to first year medical students at the private college "Médical Sciences".

2009 Lecture entitled " Cell differentiation and osteogenesis "(Master's degree, UB2)

2010 Original lecture entitled " Bioprinting approach in Tissue Engineering "(Master's degree, ENSCPB)

2011 Original lecture entitled " Bioprinting approach in Tissue Engineering "(Master's degree ENSCPB)

Master student supervision (100%):

_ Master entitled "Biomatériaux et Dispositifs Médicaux" (UB2). Head supervisors: Pr. Laurence Bordenave (UB2) and Pr. Michel Mesnard (UB1).

8 week training (2009) to elaborate a synthetic bibliographic report about " the concepts of angiogenesis, and the bioprinting materials for microvasculature engineering . The objective was to propose an original strategy based on bioprinting to fabricate blood vessels.

_ Master training ERASMUS (march to may 2010). This work has been reported in the journal Applied Surface Science (referred in the list of publications below).

Reviewing activities:

Reviewer for peer-reviewed journals: Biofabrication, Tissue Engineering, Biomedical Engineering Online.

Publications:

Catros S, Fricain J-C, Guillotin B, Pippenger B, Bareille R, Rémy M, Lebraud E, Desbats B, Amédée J and Guillemot F. Biofabrication. In revision.

Watkins M, Grimston S, Norris J, Guillotin B, Shaw A, Beniash E, and Civitelli R. Osteoblast

Connexin43 Modulates Skeletal Architecture by Regulating Both Arms of Bone Remodeling.

MBoC. In press.

Guillotin B*, Catros S*, Bačáková M and Guillemot F. Effect of laser energy, substrate film thickness and bioink viscosity on viability of endothelial cells printed by Laser-Assisted

Bioprinting. Applied Surface Science. In press. *These two authors have equally contributed to

this work.

Zouani O, Chollet C, Guillotin B and Durrieu M-C. Differentiation of pre-osteoblast cells on

poly(ethylene terephthalate) grafted with RGD and/or BMPs mimetic peptides. Biomaterials.

2010:31:8245-8253

Guillotin B, Souquet A, Catros S, Duocastella M, Pippenger B, Bellance S, Rémy M, Bareille R, Bordenave L, Amédée J and Guillemot F. Laser-assisted bioprinting of engineered tissue with high cell density and micro scale organization. Biomaterials. 2010:31:7250-7256

Kériquel V, Guillemot F, Arnault I, Guillotin B, Miraux S, Amédée J, Fricain J-C and Catros S. In vivo high-throughput biological laser printing of nano-hydroxyapatite in mice calvarial defects. Biofabrication. 2010:2:014101

Guillemot F, Souquet A, Catros S and Guillotin B. Laser-assisted cell printing: principle, physical parameters vs. cell fate, and perspectives in tissue engineering. Nanomedicine 2010:5:507-515

Guillemot F, Souquet A, Catros S, Guillotin B, Lopez J, Faucon M, Pippenger B, Bareille R, Rémy M, Bellance S, Chabassier P, Fricain J-C and Amédée J. High-throughput laser printing

of cells and biomaterials for tissue engineering. Acta Biomaterialia 2010:6:2494-2500

Trouillas M, Saucourt C, Guillotin B, Gauthereau X, Ding L, Buchholz F, Doss M-X,

Sachinidis A, Hescheler J, Hummel O, Hubner N, Kolde R, Vilo J, Schulz H and Bœuf H. Three LIF-dependent signatures and “competency genes” identified by gene profiling studies in

mouse ES cells and early derivatives. BMC Genomics. 2009:10:73.

Guillotin B, Bareille R, Bourget C, Bordenave L and Amédée J. Interaction between human umbilical vein endothelial cells and human osteoprogenitors triggers pleiotropic effect that may

support osteoblastic function. Bone. 2008:42:1080-1091.

Guillotin B, Bourget C, Remy-Zolgadri M, Bareille R, Fernandez P, Conrad V and Amedee-Vilamitjana J. Human primary endothelial cells stimulate human osteoprogenitor cell

differentiation. Cell Physiol Biochem. 2004:14:325-32

Villars F, Guillotin B, Amédée T, Dutoya S, Bordenave L, Bareille R and Amédée J. Effect of human endothelial cells on human osteoprogenitor cell differentiation needs heterotypic gap

junction communication. Am. J. Physiol Cell Physiol. 2001:282:C775-85 Review :

Guillotin B and Guillemot F. Cell manipulation technologies for organotypic tissue fabrication. Trends in biotechnology. Proposal accepted.

Invited reviews :

Trouillas M, Saucourt C, Guillotin B, Gauthereau X, Taupin J-L, Jean François Moreau J-F, and Hélène Bœuf H. The pleiotropic LIF cytokine: still looking for adulthood. Eur Cytokine Netw. 2009:20:51-62

Amédée J, Guillotin B, Pallu S, Bareille R, Guignandon A, Bourget Ch, Durrieu MC, Bordenave L. Functionalization of biomaterials and cell to cell communication. Biomed Mater Eng. 2006

Book chapter:

Guillemot F, Guillotin B, Catros S, Souquet A, Mezel C, Keriquel V, Hallo L, Fricain JC and Amédée J. High-Throughput Biological Laser Printing : Droplet Ejection Mechanism,

Integration of a Dedicated Workstation and Bioprinting of Cells and Biomaterials. Published in

"Cell and Organ Printing" (Editors: Bradley R. Ringeisen, Barry J. Spargo, and Peter Wu), Springer, 2010.

Recently invited to contribute a chapter on laser assisted cell printing (Guillotin B and Guillemot F) for the book entitled "Biofabrication for Tissue Science and Engineering” (Forgacs G and Sun W co-editors).

Oral communications and posters: International meetings:

•

F. Guillemot, B. Guillotin, S. Catros, A. Souquet, V. Kériquel, A. Fontaine, JC. Fricain.

Laser-Assisted Bioprinting for microfabrication. Oral communication

• Tissue Engineering & Regenerative Medicine Society (TERMIS 2010, Galway, Ireland)

S. Catros, S. Ziane, A. Nandakumar, B. Guillotin, L. Moroni, C. A. Van Blitterswijk, O. Chassande, B. Rousseau, R. Bareille, C. Lalande, J. Amédée, J-C. Fricain, F. Guillemot. Laser

Assisted Bioprinting on PCL Electrospun Biopapers. Oral communication

B. Guillotin, A. Souquet, S. Catros, R. Bareille, M. Rémy, J. Amédée, F. Guillemot. Laser

assisted bioprinting of engineered tissue. Poster

• International symposium on laser precision microfabrication (LPM 2010, Stuttgard, Germany)

A. Souquet, B. Guillotin, S. Catros, C. Mézel, L. Hallo, F. Guillemot. Laser assisted bioprinting of cells and biomaterials : development of a dedicated workstation, experimental

results and jet formation modeling. Oral communication

• American Society for Bone and Mineral Research (ASBMR 2010, Toronto, Canada)

M. Watkins, S. Grimston, J. Norris, B. Guillotin, R. Civitelli. A Gja1 Mutant with Dominant-Negative Action in the Osteogenic Lineage is Sufficient to Cause a Skeletal Phenotype

Resembling Oculodentodigital Dysplasia. Oral communication

• Laser Precision Microfabrication (LPM 2010, Stuttgart, Germany)

A. Souquet, B. Guillotin, S. Catros, C. Mézel, L. Hallo and F. Guillemot. Laser assisted bioprinting of cells and biomaterials: development of a dedicated workstation, experimental

• European Society for Biomaterials (ESB 2009, Lausanne, Switzerland)

O. Zouani, C. Chollet, B. Guillotin, MC. Durrieu. Mechanism of BMP induced osteogenesis

and design of peptides mimeting the activation of their receptors. Poster

• 2nd TERMIS World Congress (2009, Séoul, South Korea)

F. Guillemot, A. Souquet, B. Guillotin, S. Catros, C. Chollet, R. Bareille, M. Rémy, JC Fricain, J. Amédée. High-throughput biological laser printing for tissue engineering. Oral communication

S. Catros, JC Fricain, B. Guillotin, B. Pippenger, R. Bareille, J. Amédée, F. Guillemot.

High-throughput biological laser printing for bone tissue engineering. Oral communication

• China-Europe Symposium on Biomaterials in Regenerative Medicine (2009, Barcelone, Spain)

F. Guillemot, A. Souquet, B. Guillotin, S. Catros, R. Bareille, M. Rémy, JC. Fricain, J. Amédée. High-throughput laser printing of cells and biomaterials for bone tissue engineering.

Oral communication

• Bioprinting and Biofabrication in Bordeaux (3B09 2009, Bordeaux – organisateur

http://www.teal.u-bordeaux2.fr/)

B. Guillotin, A. Souquet, B. Pippenger, S. Bellance, M. Rémy, R. Bareille, J. Amédée, F. Guillemot. Tissue Engineering Assisted by Laser : high throughput, high resolution of cell

printing. Oral communication

• The Biology of Stem Cells (2008, Paris, France)

C Saucourt, B Guillotin, X Gauthereau, M Trouillas, H Boeuf. LIF Transcriptome and

functional tests in mouse embryonic stem cells. Poster

• American Society for Bone and Mineral Research (ASBMR 2006, Philadelphia, USA) Poster

and

• 7th _{Advanced Summer Course in Cell-Material Interactions (2006, Porto, Portugal) Oral}

communication.

M Grellier, R Bareille, C Bourget, B Guillotin, J Amédée. Human osteoprogenitor cells regulate Human endothelial cell phenotype in co-culture.

• American Society for Bone and Mineral Research (ASBMR 2005, Nashville, USA) :

M. Watkins, J. Screen, B. Guillotin, R. Civitelli. Gene Array Analysis of Connexin43 Null

Osteoblasts. Poster.

• Symposium of 15th _{Interdisciplinary Research Conference on Biomaterials (2005, Shangaï,}

Chine)

J Amédée, S Pallu, B Guillotin, R Bareille, MC Durrieu, L Bordenave, C Baquey. RGD-containing peptides may activate osteoprogenitor cells and bone regeneration in addition to

promote cooperation with vascular cells. Oral communication.

• American Society for Bone and Mineral Research (ASBMR 2002, San Antonio, USA) :

B. Guillotin, F. Villars, S. Pallu, R. Bareille, V.Conrad, J. Amédée. A method to separate human umbilical vein endothelial cells (HUVEC) from human bone marrow stromal cells

(HBMSC) after co-culture ; analysis of HUVEC effects on HBMSC differentiation.. Poster.

• American Society for Bone and Mineral Research (ASBMR 2001, Phoenix, USA):

B. Guillotin, F. Villars, R. Bareille, L. Bordenave, J. Amédée. Function of Connexin 43 and Connexin 45 in the Reciprocal Interaction between Human Endothelial Cells and Human

Bone Marrow Stromal Cells. Poster.

B. Guillotin, F. Villars, R. Bareille, L. Bordenave, J. Amédée. Reciprocal Interaction between Human Endothelial Cells and Human Bone Marrow Stromal Cells seems to be

mediated by the Production of Nitric Oxide. Poster. National meetings :

• Young Investigator Conference of "Institut Européen de Chimie et de Biologie " (IECB 2009,

Bordeaux).

B. Guillotin, A. Souquet, B. Pippenger, M. Rémy, R. Bareille, J. Amédée, F. Guillemot.

Tissue Engineering Assisted by Laser : how to achieve high resolution of cell printing. Poster.

• Graduating student Conference of Washington University (Saint Louis, 2005)

Andrew Shearer, Bertrand Guillotin, Roberto Civitelli. The role of Connexin43 in

osteoblastic differentiation and the genetic disease oculodentodigital dysplasia. Poster.

• Journée de l’école doctorale (Arcachon 2004) :

B. Guillotin, R. Bareille, C. Bourget, L. Bordenave, J. Amédée. Les cellules endothéliales de veine ombilicale humaines (HUVEC) modifient le phénotype des ostéoprogéniteurs humains

(HOP) en co-culture. Oral communication.

• Journées Françaises de Biologie des Tissus Minéralisés (JFBTM 2004, Nice):

B. Guillotin, R. Bareille, C. Bourget, L. Bordenave, J. Amédée. Les cellules endothéliales humaines de veine ombilicale (HUVEC) modifient le phénotype des ostéoprogéniteurs

humains (HOP) après co-culture. Awarded oral communication.

• Journées Françaises de Biologie des Tissus Minéralisés (JFBTM 2002, Paris):

B. Guillotin, F. Villars, S. Pallu, R. Bareille, V.Conrad, J. Amédée. Mise au point d'une méthode de séparation des cellules endothéliales humaines de veine ombilicale (HUVEC) et des ostéoprogéniteurs humains (HBMSC) après co-culture en vue d'analyser l'effet des

HUVEC sur la différenciation des HBMSC. Awarded oral communication.

• Journées Françaises de Biologie des Tissus Minéralisés (JFBTM 2001, Strasbourg):

B. Guillotin, F. Villars, R. Bareille, L. Bordenave, J. Amédée. Implication des Connexines 43 et 45 dans la Différenciation des Ostéoprogéniteurs Humains par les cellules endothéliales

Humaines en co-culture directe. Poster.

B. Guillotin, F. Villars, R. Bareille, L. Bordenave, J. Amédée. Rôle des Jonctions de Type "Gap" entre les Cellules Stromales de la Moelle Osseuse Humaines et les Cellules

Endothéliales Humaines : Etude de la Signalisation Intracellulaire. Awarded oral