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Three photon light sheet fluorescence microscopy

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Figure

Fig. 1.Published by Comparison between 2P- and 3P-LSFM. Axial maxi-mum intensity projections of 3D stacks of images of 1 µm bluefluorescing microspheres embedded in agarose under (a) 2Pexcitation at 700 nm and (b) 3P excitation at 1000 nm
Fig. 2. HEK 293 T17 cellular spheroids labeled with PURE-(d-g) Single XY planes imaged with 2P- (d, e) and 3P-LSFMBLU™ Hoechst 33342 nuclear staining dye imaged with 2P-and 3P-LSFM
Fig. 3. Characterization of LSFM with Bessel beam illumina-White lines indicate the isosurface at 5% contrast

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