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Expanding the Toolkit for Synthetic Biology: Frameworks for Native like Non natural Gene Circuits

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Figure

Figure 2.1: Workflow for generating novel sensors. The in vitro TX-TL platform allows forthe rapid screening of sequences selected with the help of computational protein design
Figure 2.2: QacR bound to DNA. Cartoon representation of a crystal structure of qacR (PDBID: 1JT0), a tetR-family repressor, bound to DNA
Figure 2.3: Computationally aided selection of qacR mutants. (a) PDB structures of the non-ligand bound (cyan, PDB ID: 1JTO) and ligand bound (magenta, PDB ID: 3BQZ) conformations ofqacR
Figure 2.5: TX-TL allows us to prototype circuits in vitro (a) TX-TL contains the transcrip-tional and translational machinery allowing you to express proteins in the reaction
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