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Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections

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Figure

Figure 2 Illustration of the recovery process for antigen-retrieval buffer in the micro-sized chamber
Figure 3 Number of identified proteins in (A) FFPE samplesand (B) supernatants. FFPE samples underwent HIAR with andwithout micro-sized chamber in antigen-retrieval buffer at 90°C or110°C
Figure 4 Amount of supernatant recovered from micro-sizedchamber after HIAR. A total of 120 μl of antigen-retrieval bufferwas sealed in the micro-sized chamber
Figure 5 Characterization of the proteins identified in the non-retrieved and retrieved FFPE sample and the supernatantdiagram comparing the number of proteins identified in the above 3 samples

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