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Simple allele-discriminating PCR for cost-effective and rapid genotyping and mapping

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Figure

Table 1: The strength of destabilization for all combinations of nucleotide pairing
Figure 1Illustration of the SAP principleThe WT (SEU) sequence and the Illustration of the SAP principle
Table 2: Primer sequences for three different alleles
Figure 2SAP-based genotyping of three different mutant allelespositive amplification of a 401 bp band when the WT (LUG) primer and the SAP-based genotyping of three different mutant alleles

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