Estrus Synchronization Protocols for Cows

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Estrus Synchronization Protocols for

Cows

Cliff Lamb

University of Florida

Applied Reproductive Strategies in Beef Cattle

NCBA Convention

January 28, 2010

Estrous Synchronization Protocols

Two shot PGF

7-11 Synch

Select Synch

Ov-synch

CO-synch

MGA/PGF

MGA Select

CIDR/PGF

CO-synch+CIDR

Heat Synch

Hybrid Synch

Hybrid Synch+CIDR

One shot PGF

Presynch

_Resynch

US Beef

Semen

Sales and Custom

Collected Semen

http://www.naab-css.org/sales/table_list.html 4 x 106 _units

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http://www.nass.usda.gov

US Beef Cow Inventory

Percentage of Semen Sold and Collected

_{Compared to Beef Cow Inventory}

11%

Definitions

!Synchronization Rate:

% of females detected in estrus compared to total number synchronized.

!Conception Rate:

% of females pregnant compared to number of females inseminated.

!Pregnancy Rate:

% of females pregnant compared to total number synchronized.

2 ! Prostaglandin (PGF

_2!

) System

PGF PGF Heat detect

and AI

-14 0 144

Days from PGF Hours from PGF

Select Synch

GnRH PGF Heat detect

and AI

-7 0 144

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Ovsynch

GnRH PGF GnRH AI

-7 0 48 60-66

CO-Synch

GnRH PGF

-7 0 48

GnRH & AI

Select Synch & TAI

GnRH PGF

-7 0 72-84

GnRH & AI Heat detect & AI

2!PGF

_2!

vs. Select Synch

GnRH PGF Heat detect and AI Select Synch PGF PGF Heat detect and AI 2!PGF2! (Stevenson et al., 2000)

Item 2!PGF2! Select Synch No. of Cows 294 289 Synchronization rate, % 142/294 (48) 175/289 (61) Conception rates, % 86/142 (61) 115/175 (66) Pregnancy rates, % 86/294 (28) 115/289 (38)

(Stevenson et al., 2000)

2!PGF

_2!

vs. Select Synch

Select Synch vs. CO-Synch

GnRH PGF Heat detect and AI Select Synch GnRH PGF GnRH & AI CO-Synch (Grieger et al., 2001)

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Item Select Synch CO-Synch No. of Cows 237 223 Synchronization rate, % 130/237 (55) -Conception rates, % 93/130 (72) 106/223 (48) Pregnancy rates, % 93/237 (39) 106/223 (48) (Grieger et al., 2001)

Select Synch vs. CO-Synch

CO-Synch vs. Ovsynch

GnRH PGF GnRH _{& AI} CO-Synch

(Geary et al., 2001)

GnRH PGF GnRH AI

Ovsynch

Days relative to PGF Hours relative to PGF -7 0 48 72 (Geary et al., 2001)

CO-Synch vs. Ovsynch

PGF TAI &GnRH PGF GnRH GnRH TAI &GnRH -7 0 48 Days relative to PGF Hours relative to PGF

CO-Synch

CO-Synch+CIDR CIDR

CO-synch vs. CO-synch+CIDR

(Lamb et al., 2001)

First Service Pregnancy Rates

Treatments

(Lamb et al., 2001)

Effect of BCS on Pregnancy Rates

(Lamb et al., 2001) Body condition score

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PGF TAI &GnRH Detect estrus & AI PGF TAI &GnRH

PGF

PGF TAI &GnRH Detect estrus & AI PGF TAI &GnRH

Detect estrus & AI GnRH GnRH GnRH GnRH TAI & GnRH -18 to -16 -7 0 60 84 B B

Days relative to PGF Hours relative to PGF

CIDR-PG & TAI

CO-Synch

CO-Synch+CIDR

Select Synch & TAI

Select Synch+CIDR & TAI

CIDR

Effect of Location on Pregnancy Rates

Locations

(Larson et al., 2006)

Pregnancy Rates after Estrus

Detection Alone vs TAI

Treatment P < 0.05 a b c a,b b 53 43 54 ₅₃ 58 (Larson et al., 2006) PGF GnRH TAI &GnRH -5 d 0 8±2h 72h CIDR (Day et al., 2008)

5-day CO-Synch + CIDR

PGF

GnRH TAI &GnRH

-7 d 0 60-66h CIDR

7-day CO-Synch + CIDR

PGF

GnRH TAI &GnRH

-5 d 0 8±2h 72h CIDR

5-day CO-Synch + CIDR

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PGF GnRH TAI &GnRH -7 d 0 60-66h CIDR PGF GnRH TAI &GnRH -5 d 0 8±2h 72h CIDR PGF 7-day CO-Synch+CIDR 5-day CO-Synch+CIDR

Distribution of Estrus for 5-day and

7-day CO-Synch + CIDR protocols

(Wilson et al., 2010)

Pregnancy rate to 7-day

CO-Synch + CIDR protocol

n > 7,000 n > 2,600

Pregnancy rate to 5-day

CO-Synch + CIDR protocol

n > 2.100 n > 2,000

Control n = 285

CIDR Natural mating

GnRH PGF TAI + GnRH TAI n = 263 Natural mating

Effects of a fixed-time AI system on

calving distribution

(Rodgers, 2007)

Calving Distribution of Cows Calving

after TAI or Bull breeding

44.1%

vs. 24.7%

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Calving Distribution after

Synchronization of Estrous

(Larson et al., 2006)

Does the use of the CO-Synch + CIDR

Protocol delay return to estrus?

438 suckled cows were estrous synchronized using

the 7-day CO-Synch + CIDR Protocol.

PGF GnRH TAI &_GnRH CIDR (Mercadante et al., 2010) n=275 n=85 n=37 n=34

90% Overall pregnancy rate

??

Calving Distribution

(Mercadante et al., 2010)

97.5% return to estrus rate!!

Estrus response of cows following the

7-day CO-Synch+CIDR protocol

Average Interval to estrus = 20.9 days (Mercadante et al., 2010)

Acknowledgements

People

Dr. Carl Dahlen Dr. Jamie Larson Mr. Guilherme Marquezini Dr. Jeff Stevenson Graduate Students Technical staff Support staff Collaborators Co-authors Beef cattle producers

Funding and Product

Support

ABS Global, Inc. Blandin Foundation Intervet Animal Health IVX/Teva Animal Health

Merial Animal Health MN-AURI

NAAB Pfizer Animal Health

Select Sires, Inc. U of FL U of MN USDA-CSREES

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Thank You!

Contact Information: Cliff Lamb University of Florida 3925 HWY 71 Marianna, FL 32446 Tel: 850-394-9124 Email: [email protected]