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Systematic analysis of the kalimantacin assembly line NRPS module using an adapted targeted mutagenesis approach

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Academic year: 2020

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Figure

Figure 1. Biosynthetic pathway and structure of kalimantacin. Biosynthesis of kalimantacin is initiated in the loading module by the acyltransferase BatH, which loads an acetyl-CoA unit onto the first ACP
Table 1. Overview of adapted specificity- conferring codes for use in of the Phe- incorporating A domain in GrsA and the Gly- incorporating A domain in Bat2mod1 are illustrated
Figure 2. Ligation independent cloning strategy. Flanking regions (350 bp and 500 bp) of the 10 AA containing active site were amplified from genomic DNA with tailed primers, introducing restriction sites that enabled restriction and ligation into the pUC1
Figure 3. Bioassay with kalimantacin and analogues. (A). Halo formation by extracts of mutant strains

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