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sNASP inhibits TLR signaling to regulate immune response in sepsis

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Figure 1. sNASP inhibits TLR4-induced NF-κB activation through TRAF6. (A) Immunoprecipitation (IP) of Flag-TRAF6 (with anti-Flag agarose) from HEK293 cells transiently transfected with Flag-TRAF6 plus GFP-tNASP (+), GFP-tNASP (+), or empty vector (–), foll
Figure 2. sNASP inhibits LPS-induced proinflammatory cytokine production. Expression of TNF-α and IL-6 in Raw264.7 cell lines transduced with EV or GFP-tagged sNASP (A) or EV or siNASP (B) and stimulated with LPS
Figure 3. Phosphorylation of sNASP regulates its interaction with TRAF6 and affects cytokine production
Figure 4. sNASP phosphorylation was observed following TLR-1, -2, -5, and -6 signaling
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