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Chemical analysis of multicellular tumour spheroids

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Figure

Fig. 1MTS organisation and radial changes that are established or pre-dicted (adapted from Lin R
Fig. 2Examples of methods of MTS formation: (a) MTS being grownusing the basic hanging drop technique, (b) MTS growing in spinnerflasks, (c) MTS being grown on microcarrier beads and (d) cells (red)growing in a scaffold of alginate gel (green), an example of the start ofMTS growth in a scaffold of NovaMatrix-3D™.
Fig. 3(a) MTS imaged using a light microscope; (b) a cross sectionthrough the centre of an MTS using OCT; (c) an OPT image of an MTSderived from a primary cancer specimen where blue core = implantedMTS, green = outgrowth into culture in collagen and red = positiveimmunoreactivity for Her2; and (d) LSM image of a MTS.8
Fig. 4comparison of the morphology of cells growing in (a) monolayerculture to (b) cells growing as MTS and (c) a magnified area of cellsgrowing as MTS.
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