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From Single Cell to Whole Body: Developing a Molecular Neuroscience Toolkit

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Figure

Fig. 1. Schematic of screen for GR spectral tuning. Briefly, mutant GR libraries were transformed into E
Fig. 3. Spectrally shifted variants identified by site-saturation mutagenesis and recombination
Fig. 4.  Relationship between hydrophobicity and residue distance from the Schiff base (K257) for blue- and red-shifting mutations
Fig. 8.  Fluorescent properties of GR variants measured in live E. coli. (a) The AUC was computed from the excitation spectra of 70 unique GR, ranked-ordered, and color-coded according to spectral shift [technical replicates of GR(D121N), a red-shifted var
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