• No results found

Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage

N/A
N/A
Protected

Academic year: 2020

Share "Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage"

Copied!
17
0
0

Loading.... (view fulltext now)

Full text

Loading

Figure

Fig. 1 SpCas9 variants employed in these studies. Schematics depicting the main features of the wild type and the five mutant variants ofSpCas9 used: each protein sequence is flanked by a nuclear localization signal (NLS) at both ends and is preceded by a
Fig. 3 Side-by-side comparison of SpCas9 variants programmed with perfectly matching sgRNAs reveal a target-selectivity ranking among thetargets examined in Fig
Fig. 4 Cleavability ranking of the targets by nuclease variant as well as fidelity ranking (eSpCas9 < SpCas9-HF1 < HeFSpCas9) of the nucleases on these
Fig. 5 Variant HeFm2SpCas9 exhibits target selectivity and fidelity between those of SpCas9-HF1 and HeFSpCas9
+2

References

Related documents

This essay asserts that to effectively degrade and ultimately destroy the Islamic State of Iraq and Syria (ISIS), and to topple the Bashar al-Assad’s regime, the international

It was decided that with the presence of such significant red flag signs that she should undergo advanced imaging, in this case an MRI, that revealed an underlying malignancy, which

Standardization of herbal raw drugs include passport data of raw plant drugs, botanical authentification, microscopic &amp; molecular examination, identification of

The analysis of ground water samples being collected from the different places of Gorakhpur city and all water quality parameters (pH, electrical conductivity,

In our study, consumption of high zinc biofortified wheat flour for 6 months compared to low zinc bioforti- fied wheat resulted in statistically significant reduction in days

3: The effect of PTU-exposure (1.5, 3, and 6 ppm) and levothyroxine therapy (Hypo 6 ppm + Levo) on the distance moved (A) and the escape latency (B) of the male

19% serve a county. Fourteen per cent of the centers provide service for adjoining states in addition to the states in which they are located; usually these adjoining states have

Aim: The aim of this study was to determine the prevalence of GBS colonization among HIV positive and HIV negative pregnant women in relation to CD4 cell