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(1)

Anticancer

Drugs ?

Dr.lokendra Sharma

Associate Professor

(2)

Advances in Cancer Chemotherapy

Treatment options of cancer:?

• No Treatment: Before 1940

• Surgery: before 1955

• Radiotherapy: 1955~1965

• Chemotherapy: after 1965

(3)

Cell Cycle

Cell Cycle Specific Agents

• Antimetabolites • Bleomycin

• Podophyllin Alkaloids • Plant Alkaloids

Cell Cycle Non-Specific Agents

• Alkylating Agents • Antibiotics

•Cisplatin

(4)

Chemotherapy

Cell cycle effects of anticancer drugs

CCS

Drugs

CCS Drugs

CCNS Drugs

G

1

- S

Etoposide

Platinum compounds

S

Antimetabolites

Alkylating agents

G

2

– M

Bleomycin

Etoposide

(Ref Harrison

17

th

/525)

Anthracyclines

Dactinomycin

M

Vinca alkaloids

Taxanes

Ixabepilone

Estramustine

Mitomycin

Camptothecins

(5)

Goals of Therapy ?

Cure or induce prolonged

‘remission’

so that all macroscopic and

microscopic features of the cancer

disappear

Acute Lymphoblastic Leukaemia

Wilm`s tumor, Ewing`s sarcoma etc.

In children, Hodgekin`s lymphoma, testicular

teratoma and choriocarcinoma

(6)

Goals of Therapy ?

Palliation:

Shrinkage of evident tumour,

Alleviation of symptoms and

prolongation of life

Breast cancer, ovarian cancer,

endometrial carcinoma,

CLL, CML,

Small cell cancer of lung and

(7)

Goals of Therapy ?

Insensitive or less sensitive but life

may be prolonged

Cancer esophagus, cancer stomach,

sq. cell carcinoma of lung,

melanoma,

pancreatic cancer,

myeloma,

(8)

Aim of Therapy – contd.

• Adjuvant therapy:

– For mopping up of residual cancer cells

including metastases after Surgery,

– Radiation and immunotherapy etc.

• Routinely used now

(9)

General Principles

Analogous with Bacterial chemotherapy –

differences are

Selectivity of drugs is limited

No or less defence mechanism – Cytokines

adjuvant now

All malignant cells must be killed to stop

progemy

Subpopulation cells differ in rate of

proliferation and susceptibility to

chemotherapy

(10)

General Principles

Drug regimens or combined cycle

therapy after radiation or surgery

Complete remission should be the

goal

Formerly single drug – now 2-5

drugs in intermittent pulses

Total tumour cell kill –

(11)

COMBINATION CHEMOTHERAPY

- SYNERGISTIC ?

Drugs which are effective when used

alone

Different mechanism of action

Differing toxicities

Different mechanism of toxicities

Synergistic biochemical interactions

Optimal schedule by trial and error

method

(12)

Classification ?

 According to chemical structure and sources of drugs

– Alkylating Agents, Antimetabolite, Antibiotics, Plant Extracts,

Hormones and Others

 According to biochemistry mechanisms of anticancer action:

– Block nucleic acid biosynthesis

– Direct influence the structure and function of DNA – Interfere transcription and block RNA synthesis – Interfere protein synthesis and function

– Influence hormone homeostasis

 According to the cycle or phase specificity of the drug:

– Cell cycle nonspecific agents (CCNSA) & Cell cycle specific agents

(CCSA)

(13)

Block nucleic acid (DNA, RNA)

biosynthesis

Antimetabolites:

• Folic Acid Antagonist: inhibit dihydrofolate

reductase (methotrexate)

• Pyrimidine Antagonist: inhibit thymidylate

synthetase (fluorouracil) ; inhibit DNA polymerase

(cytarabine)

• Purine Antagonist: inhibit interconversion of purine

nucleotide (6-mercaptopurine and 6-Thioguanine)

• Ribonucleoside Diphosphate Reductase

(14)

Influence :Structure & Function of

DNA

• Alkylating Agent: mechlorethamine,

cyclophosphamide, ifosfamide, chlorambucil,

Mephalan, Busulfan, Nitrosoureas and Thio-TEPA

• Platinum: cis-platinium, carboplatin and

imatinib

• Antibiotic: bleomycin and mitomycin C

• Topoismerase inhibitor: camptothecin

analogues and podophyllotoxin and antibiotics

like actinomycin D, daunorubicin and

(15)

Sites of Antineoplastic Action ?

(16)

Clinical Considerations ?

Early intensive start …. helpful

Complete remission….. goal

Combined chemotherapy useful

…..delayed emergence of resistance

Combined chemotherapy …..curative

Treatment must continue past the

time when cancer cells can be

detected using conventional

techniques

(17)

Resistance ?

Intrinsic:

malignant melanoma, renal cell cancer, and

brain cancer, exhibit primary resistance

Acquired:

Single drug:

change in the genetic apparatus amplification or

increased expression of one or more specific

genes

Multidrug resistance:

Resistance variety of drugs exposure to a single variety of drug

increased expression of a normal gene (the MDR1 gene) for a cell surface glycoprotein (P-glycoprotein) involved in drug efflux

(18)

Toxicities ?

Harmful to normal tissues too

Steep dose response curve

Low therapeutic index

Particularly harmful to rapidly

multiplying normal tissues: GI

mucosa, Bone Marrow, RE system and

gonads and hair cells

(19)

Toxicities ?

Bone marrow depression – limits treatment

Buccal mucosa erosion – high epithelial turnover

(stomatitis, bleeding gums)

GIT: Diarrhoea, shedding of mucosa, haemorrhage

Nausea, vomiting – CTZ direct stimulation

Skin: alopecia

Gonads: oligospermia, impotence, amenorrhoea

and infertility

Lymphoreticular system: Lymphocytopenia and

inhibition of lymphocyte function – loss of host

defense mechanism – susceptibility to infections

Carcinogenicity

(20)

Distinctive Toxicities of Alkylating Agents ?

Drug

Toxicity

Cyclophosphamide

Alopecia, Hemorrhage cystitis, SIADH

Ifosfamide

Hemorrhagic cystitis, SIADH

Busulfan

Pulmonary fibrosis, Hyper pigmentation,

Adrenal insufficiency

Procarbazine

Secondary leukemias, Disulfiram like

reaction, behavioral changes, CNS

depression

Cisplatin

Emesis, Nephrotoxicity, Peripheral

sensory neuropathy, ototoxicity

(21)

Countering the Toxicities ?

Intermittent therapy

Folinic acid rescue

Systemic Mesna (sodium-2-mercaptoethane

sulfonate) administration and irrigation by

acetylcysteine – detoxify toxic metabolites

Ondansetron

Hyperurecaemia: uricosuric agents like

allopurinol

Platelet and granulocyte transfusion

Granulocyte colony stimulating factors

(GM-CSF/G-CSF) – recovery of garnulocytopenia

(22)

Drugs used to prevent toxicity of Anti cancer drugs

Drug Mechanism Indications

Allopurinol Inhibit xanthine oxidase Prevent hyperuricemia from tumor lysis syndrome

Rasburicase Recombinant urate oxidase Prevent hyperuricemia from lysis Mesna Neutralizing agent Prevent hemorrhagic cystitis due to

ifosfamide and high dose cyclophosphamide

Leucovoring Replete Tetrahydrofolic acid Rescue after high dose methotrexate Amifostine Prevent radiation induced

xerostomia and Prevent radiation induced xerostomia and cisplatin induced nephrotoxicity

Dexrazoxane Iron chelator Prevent cardiotoxicity due to anthracyclines

Palifermin Keratinocyte growth factor Prevent mucositis following chemotherapy

Pilocarpine Cholinergic agonist Radiation induced xerostomia Pamidronate and

(23)

Drugs used to prevent toxicity of

Anti cancer drugs

Drug Mechanism Indications

Epoetin alpha and darbopoetin alpha

Erythropoietin Anemia

Filgrastim,

peg-filgrastim G-CSF and Febrile neutropenia prophylaxis Sargramostim GM - CHF

Oprelvekin IL-11 Thrombocytopenia Ondansetron 5-HT3 antagonist Nausea and vomiting

Granisetron Palonosetron

(24)

Interfere Protein Synthesis

Antitubulin:

vinca alkaloids (vincristine and

vinblastin) and taxanes (paclitaxel and

docetaxel)

Bind tubulin, destroy spindle to produce

mitotic arrest

Influence amino acid supply:

L-asparaginase

(25)

Some Alkylating Agents used in cancer Chemotherapy

Agent Route of

Admin. Cancer where preferred Delayed Toxicity 1. Busulphan Oral CML, PV BMD, bleeding, skin

pigmentation adrenal insufficiency, pulmonary fibrosis

2. chlorambucil Oral CLL, PV BMD, bleeding 3.

Cyclophosphamide Oral, i.v All, NHL, PV, Carcinoid tumour, Neurobalstoma BMD, bleeding, hemorrhagic, cystitis 4. Melphalan Oral Multiple myeloma BMD, Bleeding

5. Mechlorethamine i.v. Hodgkin’s disease BMD, alopecia, Diarrhea oral ulcer leukaemia

6. Cisplatin i.v. CA tests, ovary, cervix, lung, head & neck, thyroid,

Melanoma

Renal damage, ototoxicity, neuropathy, BMD

7. Dacarbazine i.v. Melanoma, Hodgkin’s disease BMD 8. Carmustine

(BCNU) i.v. Brain tumours Leukopaenia, thrombocytopaenia 9. Lomustine

(CCNU) Oral Brain tumours Leukopaenia, thrombocytopaenia 10. Thiotepa i.v. CA bladder (early) & Ovary BMD

(26)

Tyrosine Kinase Inhibitors

Drug Inhibit TK activated Indication

Axitinib VEGFR – 1,2,3 Advanced renal cell carcinoma Bosutinib Abl –bcr, src CML

Crizotinib c-MET, ALK Non small cell lung carcinoma Cabozantinib c-MET, VEGFR-2 Medullary carcinoma thyroid

Dasatinib abl -bcr CML

Erlotinib EGFR Non small cell lung carcinoma Pancreatic carcinoma

Geftinib abl-bcr, c- KIT, PDGF Non small cell lung carcinoma Imatinib her-2/neu, erb-B2 CML GIST

Lapatinib abl-bcr Breast carcinoma Nilotinib VEGFR-1,2,3 PDGFR α β

c-KIT

CML

(27)

Tyrosine Kinase Inhibitors

Drug Inhibit TK activated Indication

Regorafenib VDGFR2, TIE2 Colorectal carcinoma GIST Ruxolitinib JAK 1,2 Myelofibrosis

Sorafenib VEGFR, PDGFR RAF Renal cell carcinoma Hepatocellular carcinoma Sunitinib VEGFR, PDGFR c- KIT, FLT-3 RET Renal cell carcinoma,

Pancreatic neuroendocrine tumors GIST

Tofacitinib JAK Rheumatoid arthritis Vandetanib VEGFR, EGFR Medullary carcinoma

thyroid

(28)

Monoclonal Antibodies

S.

No. Monoclonal antibody Targeted against Indication Comments 1 Rituximab CD - 20 Non hodgkin lymphoma

2 Alemtuzumab CD - 52 Low grade lymphomas and CLL

3 Trastuzumab HER 2/neu Breast Carcinoma Can cause cardiotoxicity 4 Cetuximab and

panitumumab EGFR EGFR – positive metastatic colorectal carcinoma Cause rash, Hypomagnesemia and tnterstitial lung

disease 5 Bevacizumab VEGF Metastatic colorectal

carcinoma Combined with 5 - FU 6 Gemtuzumab CD-33 CD-33 Positive AML Linked to

calicheamicin 7 I131 – Tositumomab

Y90 – Ibritumomab

tiuxetan

CD-20 Relapsed lymphomas Conjugated with radioisotopes 8 Denileukin diftitox - Recurrentcutaneous T-cell

(29)

Therapy of choice for various cancers

S. No. Diagnosis Treatment of choice

1 All Induction: Vincristine + Prednisolone+Daunorubicin+ Asparaginase+Intrathecal Methotrexate

Consolidation: Hyper-CVAD alternated with cytarabine+Methotrexate

2 AML Cytarabine+Daunorubicin/Idarubicin

3 CML Imatinib

4 CLL FCR or Fludarabine 5 Hairy cell leukemia Cladribine

6 Hodgkin disease ABVD 7 Non hodgkin

lymphoma CHOP-R

8 Multiple Myeloma Bortezomib+Dexamethasone+Lenalidomide 9 Waldenstrom

macroglobulinemia FCR

(30)

Therapy of choice for various cancers

S. No. Diagnosis Treatment of choice

11 Non small cell lung

cancer Cisplatin + Vinorelbine ± Bevacizumab 12 Small cell lung

cancer Cisplatin + Etoposide 13 Mesothelioma Cisplatin + Pemetrexed 14 Head and neck cancer Cisplatin + 5-FU

(31)

Some antimetabolites used in cancer chemotherapy

Agent Route of admin. Cancer (s) where preferred Delayed toxicity

1. Cytarabine i.v. AML BMD, nausea, Vomiting stomatitis ataxia

(cerevellar) 2. 5- Fluorouracil i.v. Carcinoma head &

neck, Stomach colon, breast

BMD, Oral and GI ulceration, nausea, diarrhoea,

neurotoxicity, *hand and foot syndrome 3. 6-

Mercaptopuine Oral All BMD, Hyperuricaemia, immunosuppression, hepatotoxicity

4. Methotrexate Oral All, choriocarcinoma,

osteogenic sarcoma BMD, vomiting, oral & GI ulcers hepatotoxicity (acute & chronic)

(32)

Some natural products in cancer chemotherapy

Agent Cancer (s) where preferred Delayed toxicity

Antibiotics

1. Bleomycin Carcinoma testis, malignant

effusion (intracavity) Alopecia, oedema of hand, pulomonary fibrosis, stomatitis 2. Dactinomycin Wilm’s tumour Alopecia, BMD, Stomatitis, Oral

ulcer

3. Daunorubicin AML Alopecia, BMD, Cardiomyopathy 4. Doxorubicin HL, NHL, neuroblastoma,

Carcinoma thyroid, stomach, carcinoid tumouir, sarcomas, osteogenic sarcoma

Alopecia, BMD, Cardiomyopathy, stomatitis

5. Mitomycin Carcinoma stomach Thrombocytopaenia, leukopaenia 6. Streptozotocin

(sreptozocin) Insulinoma Renal damage, hypoglycaemia, hyperglycaemia, liver damage, BMD, fever eosinophilia,

(33)

Some natural products in cancer chemotherapy

Agent Cancer (s) where

preferred Delayed toxicity

Plant Alkaloids

1. Docetaxel Advance case of

carcinoma breast Neurotoxicity, fluid retention, neutropaenia 2. Etoposide Carcinoma testis,

choriocarcinoma

Alopecia, BMD

3. Paclitaxel Carcinoma breast, ovary BMD, peripheral neuritis

4. Vinblastine HD Alopecia, BMD, Loss of reflex 5. Vincristine ALL, NHL Alopecia, BMD, Peripheral

neuritis

6. Vinorelbine Carcinoma lung BMD, fatigue, constipation, hyporeflexia paresthesia

(34)

Miscellaneous agents including monoclonal antibodies in cancer chemotherapy

Agent Route of admin. Cancer(s) where used Delayed toxicity

1. Asparaginase i.v. All in child Hepatotoxicity, mental depression,

pancreatitis 2. Cisplatin i.v. CA testis, ovary,

cervix, lung, head & neck, thyroid,

melanoma

Renal damage,

otoxicity neuropathy, BMD

3. Hydroxyurea Oral CML, AML (blast

crisis) BMD

4. Mitotane Oral Adrenocortical

carcinoma Adrenal insufficiency, diarrhoea, lethargy, skin rash (transient) 5. Mitoxantrone Oral Aml BMD, cardiotoxicity,

alopecia 6. Imatinib Oral CML (chronic phase)

& blast crisis Fluid retention (periorbital and ankle oedema), diarrhoea, myalgia

7. Trastuzumab i.v. Carcinoma breast

(metaastatic) BMD, cardiomyopathy, pulm. Toxicity, cardiac

(35)

Hormones, their antagonists and related agents in

cancer chemotherapy

Agent Route of admin Cancer(s) where

preferred Delayed toxicity Corticosteroids

Hydrocortisone Prednisone

Oral

Oral All, CLL, NHL, HL Multiple myeloma Fluid retention,Hypertension, diabetes mellitus, susceptibility to infection, moon face

Androgens

Testosterone i.m. Premenopausal breast cancer (oestrogen receptor positive) Fluid retention masculinization Oestrogens Diethylstilboesterol Ethinyloestradiol Oral

Oral Carcinoma prostate,Postmenopausal breast cancer (oestrogen receptors negative) Feminization, Fluid retention Progestins Hydroxyprogesterone Medroxyprogesterone i.m.

(36)

Influence hormone

homeostasis

Estrogens and estrogen antagonistic drug

(EE, SERM-tamoxifene)

Androgens and androgen antagonistic

drug (flutamide and bicalutamide)

Progestogen drug (hydroxyprogesterone)

Glucocorticoid drug (prednisolone and

others)

Gonadotropin-releasing hormone

inhibitor: nafarelin, triptorelin

aromatase inhibitor: Letrozole and

(37)

Hormones, their antagonists and related agents in

cancer chemotherapy

Agent Route of admin Cancer(s) where

preferred Delayed toxicity Antiandrogen

Flutamide Oral Carcinoma prostate None

Antiandrogen

Tamoxifen Oral Carcinoma breast (early stage, metastatic after surgery)

None

Others GnRH Agonist Goserelin

Leuprolide s.c)s.c.)

Carcinoma prostate None

Aromatase Nhibitors

Aminogulutethimide Oral Metastatic breast cancer None Peptide hormone

(38)

Choice of drug in some malignancies where the response of chemotherapy is very good

Cancer Treatment of choice

1. Acute lymphocytic leukaemia Induction: Vincristine + presnisone

Maintenance: Methotrexate + Mercaptopurine + Cyclophosphamide

2. Hodgkin’s disease stage I and II

Stage III and IV RadiotherapyDoxorubicin +

bleomycin+vinblastine+dacarbazine

3. Non Hodgkin’s disease Cyclophosphamide + doxorubicin + vincristine + prednisolone

4. Choriocarcinoma Methotrexate + folic acid or cisplatin + etoposide 5. Cancer testis Bleomycin + cisplatin+ etoposide

6. Wilm’s tumour Surgery + radiotherapy followed by vincristine + dactinomycin

(39)

Choice of drug in some malignancies where the

response of chemotherapy is good

Cancer

Treatment of choice

1. Acute myeloid leukaemia Cytarabine + idarubicin/daunorubicin 2. Chronic lymphocytic

leukaemia Chlorambucil + prednisone (if indicated) + fludarabine or cytarabine alone or in combination with other drugs

3. Chronic myelogenous

leukaemia Busulfan or interferon, imatinib (bone marrow transplatation in selected patients) 4. Multiple myeloma Melphalan + prednisone

5. * Carcinoma breast stage 1 Tomoxifen after breast surgery 6. Endometrial carcinoma Progestins or tamoxifen

7. Carcinoma cervix Radiation + cisplatin (localized), cisplatin/carboplatin (metastatic)

8. Carcinoma prostate GnRh agonist or oestrogen + androgen anatagonist (flutamide)

(40)

Choice of drug in some malignancies where the

response of chemotherapy is average

Cancer Treatment of choice 1. Carcinoma breast stage II to

IV Cyclophosphamide + methotrexate + 5-FU or Transtuzumab + prednisone + antioestrogen 2. Carcinoma ovary Cisplatin or carboplatin + paclitaxel + interferom 3. Carcinoma thyroid Radioidine (I131), doxorubicin, cisplatin

4. Carcinoma stomach 5-FU + doxorubicin + mitomycin 5. Carcinoma colon 5-FU + leucovorin + irinotecan

6. Osteogenic sarcoma Doxorubicin or methotrexate with leucovorin after surgery

(41)

Choice of drug in some malignancies where the

response of chemotherapy in unsatisfactory

Cancer

Treatment of choice

1. Carcinoma lung

Etopise + cisplatin, vinorelbine

2. Carcinoma head and

neck

5-fu+cisplatin or cisplatin + paclitaxel

3. Carcinoma adrenal

gland

Mitotane

4. Carcinoid tumour

Doxorubicin + cyclophospamide or

5-FU + octreotide

5. Polycythaemia vera

Busulfan, chlorambucil or

cyclophospamide

(42)

Alkylating Agents

Mechanism of Action:

• Nitrogen mustards inhibit cell reproduction

binding irreversibly nucleic acids (DNA)

• After alkylation, DNA is unable to replicate

• no synthesize proteins and essential cell

metabolites

• Consequently, cell reproduction inhibited cell

eventually dies inability maintain metabolic

functions.

(43)

Nitrogen Mustards

• Mechlorethamine:

– Uses: IV

– MOPP (Mechlorethamine – oncovine-prednisolone and procarbazine) in Hodgekin`s lymphoma and disease – ADRs: Severe Vomiting, myelo and immunosuppression – Extravasation – severe local toxicity

• Cycolphosphamide:

– Transformed active aldophosphamide and phospharamide

– orally

– Used Hodgkin's lymphoma, breast and ovary cancers – Ifosphamide longer half life and used mainly testicular

(44)

Nitrogen Mustards – contd.

• Chlorambucil: orally, active against

lymphoid tissues (Ch. Lymphatic leukaemia

and non-Hodgkin's lymphoma)

• Busulfan: orally, active against CML

• Carmustine: IV, effective against brain

tumors and Hodgkin's lymphoma

• Dacarbazine: Different from other alkylating

agents – action against RNA and protein

synthesis

(45)

Antimetabolites

Folic acid Antagonists: MTX

Purine Antagonists: 6MP and 6TG

Pyrimidine

Antagonists:

5FU

and

cytarabine

General Characteristics:

Antimetabolites S phase-specific drugs

structural analogues of essential metabolites and

that interfere with DNA synthesis.

(46)

Methotrexate – Folate

Antagonist

• MOA:

– Structures MTX and folic acid similar

– MTX actively transported mammalian cells and

inhibits dihydrofolate reductase

– the enzyme that normally converts dietary folate

to the tetrahydrofolate form required for thymidine

and purine synthesis

• Leucovorin rescue:

– Administered as a plan in MTX therapy

– Leucovorin (Folinic acid) is directly converted to

tetrahydrofolic acid - production of DNA cellular

protein inspite of presence of MTX

(47)

Methotrexate – contd.

• Kinetics:

– orally/IM /IV intrathecally , good oral

absorption

– CSF entry - intrathecal

• Indications:

– Choriocarinoma - was the first demonstration of curative chemotherapy

– Tumors of head and neck – Breast cancer

– Acue lymphatic leukemia

(48)

Purine Antagonists

– 6MP,

6TG

6-Mercapapurine (6-MP) and others

• Exact mechanisms uncertain

– inhibit

purine base synthesis

• Used in childhood Acute lymphatic

Leukaemia for maintenance and remission

• combination MTX choriocarcinoma

• Metabolized xanthine oxidase (inhibited

by allopurinol) and allopurinol dose has to

be adjusted to

½ or 1/4

th

• Well tolerated, mild myelosuppression ,

(49)

Antimetabolites (Pyrimidine

Antagonists) - 5 FU

• MOA:

– Fluorouracil analogue of thymine

– Converted to 5-fluoro-2deoxy-uridine

monophosphate (5-FdUMP)

– 5-FdUMP inhibits thymidylate synthase and

blocks conversion of deoxyuridilic acid to

deoxythymidylic acid

– failure of DNA

synthesis

• Indications: solid tumors, especially

breast, colorectal, and gastric tumors

and squamous cell tumors of the head

and neck

(50)

Antibiotics

• Anthracyclines (doxorubicin and dau norubicin), Dactinomycin, Bleomycin, and mitomycin

• Anthracyclines:

– Enters themselves into DNA and causes DNA break – Activates TopoisomeraseII and cause break in DNA

strands

– Generates excess free radicals causing production of superoxide – damage to DNA

– Known to damage cardiac cells also (unique)

– Resistance developes due to increased eflux of drug – Uses: Doxo- Breast, ovary, lung, [prostate and acute

lymphatic leukaemia – Dauno- ALL and AML

(51)

1. Which of the following is a radioprotector?

a. Colony stimulating factor

b. Amifostine

c. Cisplatin

d. Methotrexate

(b)

2. Topical mitomycin-C is used in

a. Sturge-Weber syndrome

b. Laryngotracheal stenosis

c. Endoscopic angiofibroma

d. Skull base osteomyelitis

(52)

3. Which group of anticancer drugs Temozolomide belong to a. Oral alkylating agent

b. Antitumor Antibiotic c. Antimetabolite

d. Mitotic Spindle Inhibitor (a)

4. Methotrexate is used for the management of all of these conditions except a. Rheumatoid arthritis

b. Psoriasis

c. Sickle cell anemia d. Organ transplantation

(53)

5. Which of the following drug is used for the is treatment of sickle cell anemia? a.Hydroxyurea b. Cisplatin c. Paclitaxel d. Carboplatin (a)

6. Use of tamoxifen in carcinoma of breast patients does not lead to the following side effects

a. Thromboembolic events b. Endometrial carcinoma c. Cataract

d. Cancer in opposite breast (d)

(54)

7. All of the following are true regarding ifosfamide EXCEPT a. Metabolised by cytochrome p450 enzymes

b. Less neurotoxic than cyclophosphamide

c. Chloracetaldehyde is the metabolite of ifosfamide d. It is a nitrogen mustard

(b)

8. Alkalinisation of urine ameliorates the toxicity of which of the following drugs? a. Arabinoside-cytosine

b. Ifosfamide c. Cisplatin

d. Methotrexate (d)

(55)

11. Pulmonary fibrosis is seen with a. Bleomycin b. Cisplatin c. Methotrexate d. Actinomycin D (a)

12. Which of the following drug is used in the treatment of estrogen dependent breast carcinoma?

a. Tamoxifen b. Methotrexate c. Paclitaxel

(56)

13. Methotrexate resistance is due to: a. Depletion of Folate

b. Overproduction of DHFRase

c. Overproduction of Thymidylate kinase d. Decreased DHFRase

(b)

14. Hemorrhagic cystitis is caused by a. Cyclophosphamide

b. 6 Mercaptopurine c. 5 Fluorouracil d. Busulfan

(57)

15. Thalidomide is used in all of the following except a. HIV associated peripheral neuropathy

b. HIV associated aphthous (mouth) ulcers c. Behcet syndrome

d. Erythema Nodosum Leprosum (a)

16. Most common dose-limiting toxicity of cancer chemotherapy is a. Gastrointestinal toxicity

b. Neurotoxicity

c. Bone marrow suppression d. Nephrotoxicity

(58)

17. Which of the following parameters is not monitored in a patient on methotrexate therapy?

a. Liver function tests b. Lung function test c. Eye examination d. Hemogramz

(c)

18. All of the following are true about thalidomide except

a. Used in pregnancy as anti-emetic but withdrawn due to teratogenicity

b. Can be used in multiple myeloma as primary treat ment as well as in refractory disease c. Causes euphoria and diarrhea

d. Can be used in erythema nodosum leprosum (c)

(59)

19. Which of the following drug acts by inhibiting tyrosine kinase activated by EGF receptor as well as HER2?

a. Imatinib b. Geftinib c. Erlotinib d. Lapatinib

(d)

20. Tyrosine kinase inhibitors are first line treatment in a. Gastrointestinal stromal tumors

b. Receptor mediated neuroendocrine tumors c. Breast cancer

d. Renal cell carcinoma (a)

(60)

• 21. Drug locally used for tracheal stenosis is a. Mitomycin C b. Doxorubicin c. Bleomycin d. Clindamycin (a)

22. Cetuximab (an EGFR antagonist) can be used in a. Palliation in head and neck cancer

b. Anal canal carcinoma c. Gastric carcinoma d. Lung carcinoma

(61)

23. Most emetogenic anticancer drug is a. Cisplatin

b. Carboplatin

c. High dose cyclophosphamide d. High dose methotrexate

(a)

24. Cerebellar toxicity is seen with a. Cisplatin

b. Cytarabine c. Bleomycin

d. Actinomycin D (b)

(62)

25. All are alkylating agents, except a. 5-Fluorouracil b. Melphalan c. Cyclophosphamide d. Chlorambucil (a)

26. Which of the following can be given orally? a. Cytosine arabinoside

b. Cisplatin c. Doxorubicin d. Mesna

(63)

27. In treatment of osteosarcoma, all of the following are used EXCEPT a. High dose methotrexate

b. Cyclophosphamide c. Vincristine

d. Doxorubicin (c)

28. 'Hand and Foot' syndrome can be caused by a. Cisplatin

b. Vincristine c. Capecitabine d. Mitomycin-C

(64)

29. Which of the following anti-cancer drugs is cell cycle specific? a. Ifosfamide b. Melphalan c. Vinblastine d. Cyclophosphamide (c)

30. Topical mitomycin-C is used in a. Sturge-Weber syndrome

b. Laryngotracheal stenosis c. Endoscopic angiofibroma d. Skull base osteomyelitis

(65)

31. Amifostine is protective to all EXCEPT a. Salivary glands b. Skin c. CNS d. GIT (c)

32. Bleomycin toxicity affects which type of cells a. Type-I pneumocytes

b. Type-II pneumocytes c. Endothelial cells

d. Pulmonary alveolar macrophages (b)

(66)

33. SIADH is caused by all EXCEPT a. Vincristine b. Vinblastine c. Actinomycin D d. Cyclophosphamide (c)

34. Imatinib is used in the treatment of? a. Chronic myelomonocytic leukemia b. Myelodysplastic syndrome

c. Acute lymphoid leukemia

d. Gastro intestinal stromal tumors (d)

(67)

35. Sustained neutropenia is seen with? a. Vinblastine b. Cisplatin c. Carmustine d. Cyclophosphamide (c)

36. Rituximab is used in all EXCEPT a. Non Hodgkin lymphoma

b. Paroxysomal nocturnal hemoglobinurea c. Rheumatoid arthritis

d. Systemic lupus erythematosis (b)

(68)

39. Which of the following anticancer drug is excreted by lungs? a. 5-Fluorouracil b. Cyclophosphamide c. Doxorubicin d. Cisplatin (a)

40. Which of the following drugs is used for the treatment of refractoty histiocytosis?

a. High dose methotrexate b. High dose cytarabine c. Cladribine

d. Fludarabine (c)

(69)

41. Thalidomide, used for multiple myeloma, is a. Associated with diarrhea

b. Characterized by enantiomeric intercon-versions c. Metabolized extensively by hepatic CYP system d. Safe for use in pregnant females

(b)

42. A patient on treatment for leukemia, develops chest pain, pulmonary infiltrates and pleural effusion. The likely causeis.

a. Daunorubicin b. Hydroxyurea c. Cytarabine d. Tretinoin

(70)

43. Mechanism of action of paclitaxel is a. Topoisomerase inhibition

b. Increases the polymerization of tubulin c. Inhibits protein synthesis

d. Alkylation of DNA (b)

44. Which antineoplastic drug is a peptide? a. Bleomycin

b. Asparteme c. Valinomycin d. Dactinomycin

(71)

45. Leucovorin is used to decrease the toxicity of

a. Methotrexate

b. Mercaptopurine

c. Thio-TEPA

d. Cytosine arabinoside

(a)

46. All-trans-retinoic acid is used in treatment of

a. Acute promyelocytic leukemia

b. A.L.L.

c. CML

d. Transient myeloproliferative disorder

(a)

(72)

47. Treatment of choice for chronic myeloid leukemia is a. Imatinib b. Hydroxyl-urea c. Interferon-alpha d. Cytarabine (a)

48. Which of the following anticancer drugs can cause hypercoagulable state? a. 5-FU

b. L-asparaginase c. Melphalan

d. Carmustine (b)

(73)

49. Anticancer drug causing SIADH as an adverse effect is a. Vincristine b. Paclitaxel c. Dacarbazine d. Cyclophosphamide (a)

50. Which of the following anticancer drugs acts by hypomethylation? a. Gemcitabine

b. 5-FU

c. Decitabine

d. Homoharringotonine (c)

(74)

51. High dose methotrexate is used for the treatment of a. Osteosarcoma b. Rhabdomyosarcoma c. Retinoblastoma d. Ewing's sarcoma (a)

52. Which of the following drugs is topoisomerase 1 inhibitor? a. Doxorubicin

b. Irinotecan c. Etoposide d. Vincristine

(75)

53. All of the following anticancer agents cause bone marrow suppression EXCEPT a. Chlorambucil b. Daunorubicin c. Doxorubicin d. Flutamide (d)

54. All the following are hormonal agents used against breast cancer EXCEPT a. Letrozole

b. Exemestane c. Taxol

d. Tamoxifen (c)

(76)

55. Which is the most active single chemotherapeutic agent in the treatment of leiomyosarcoma? a. Adriamycin b. Daunorubicin c. Methotrexate d. Cisplatin (a) 56. Gemcitabine is effective in a. Head and neck cancers

b. Pancreatic cancer

c. Small-cell lung cancer d. Soft tissue sarcoma

(77)

57. All of the following statements about methotrexate are correct EXCEPT a. Folinic acid enhances the action of methotrexate

b. Methotrexate inhibits dihydrofolate reductase

c. Non-proliferative cells are resistant to methotrexate d. Methotrexate is used in the treatment of psoriasis

(a)

58. Mesna is given with cyclophosphamide to a. Increase absorption

b. Decreased excretion

c. Ameliorate hemorrhagic cystitis d. Decrease metabolism

(78)

59. A 35 yr old patient is having carcinoma lung with a past history of lung disease. Which of the following drugs should not be given?

a. Vinblastine b. Bleomycin c. Mithramycin d. Adriamycin

(b)

60. Arsenic is useful in the treatment of a. Acute promyelocytic leukemia

b. Myelodysplastic syndrome

c. Transient myeloproliferative disorder d. All of the above

(79)

61. Which of the following is an anti-metabolite? a. Methotrexate b. Cyclosporine c. Etoposide d. Vinblastine (a)

62. Mechanism of action of imatinib mesylate is a. Increase in metabolism of P glycoprotein

b. Blocking the action of P glycoprotein

c. Blocks the action of chimeric fusion protein of bcr abl d. Non-competitive inhibition of ATP binding site

(80)

63. Which of the following drugs is associated with untoward side effect of renal tubular damage? a. Cisplatin b. Streptozocin c. Methysergide d. Cyclophosphamide (a)

64. Which of the following chemotherapeutic agents is associated with secondary leukemia? a. Vinblastine b. Paclitaxel c. Cisplatin d. Bleomycin (c)

(81)

65. The drug imatinib acts by the inhibition of a. Tyrosine kinase b. Glutathione reductase c. Thymidylate synthetase d. Protein kinase (a)

66. The new drug pemetrexed useful in breast cancer belongs to which of the following category of the drugs?

a. Antitumor agent b. Alkylating agent c. Hormonal agent d. Antimetabolite

(82)

71. Sodium 2-mercapto ethane sulfonate is used as a protective agent in a. Radiotherapy b. Cancer chemotherapy c. Lithotripsy d. Hepatic encephalopathy (b) 72. Pulmonary fibrosis is a common complication after treatment with

a. 6-Mercaptopurine b. Vincristine

c. Bleomycin d. Adriamycin

(83)

73. A patient receiving allopurinol requires dose reduction of a. 6-Meracaptopurine b. Cyclophosphamide c. 6-Thioguanine d. Climetidine (a)

74. Which of the following are alkylating agents? a. Cyclophosphamide

b. Ifosfamide c. Methotrexate d. Vincristine

(84)

75. Anticancer drugs of plant origin is/are a. Vincristine b. Isotretinoin c. Bleomycin d. Methotrexate (a, b)

76. Alkylating agents are a. Vincristine b. Actinomycin-D c. Chlorambucil d. 5-Fluorouracil e. Cyclophosphamide (c, e)

(85)

77. Which of the following drugs are anticancer antibiotics? a. Vancomycin b. Actinomycin D c. Bleomycin d. Mithramycin e. Vincristine (b, c, d)

78. Metaphase arrest is caused by a. Griseofulvin b. Vincristine c. Paclitaxel d. Colchicine e. Etoposide (b, c, d)

(86)

79. The mechanism of anticancer action of fluorouracil is

a. Cross linking of double stranded DNA and the resulting inhibition of DNA replication and transcription

b. Cytotoxicity resulting from a metabolite that interferes with the production of dTMP c. Irreversible inhibition of dihydrofolic acid reductase

d. Selective action on DNA polymerase (b)

80. A cell cycle specific anticancer drug that acts mainly in the M phase of the cycle is a. Cisplatin

b. Etoposide c. Methotrexate d. Paclitaxel

(87)

81. Maintenance of high urinary pH is important during methotrexate treatment because

a. Bladder irritation is reduced

b. It decreases renal tubular secretion of methotrexate

c. Leucovorin toxicity is increased in a dehydrated patient d. Methotrexate is a weak acid

(d)

82. All of the following statements about methotrexate are true Except a. It is cell cycle specific and kills in the S phase

b. Its toxicity primarily affects bone marrow and epithelial structures c. Folic acid reverses its toxic effects

d. It is the drugs of choice for choriocarcinoma (c)

(88)

83. Mechanism of action of vincristine in the treatment of All is a. Inhibition of topoisomerase II to cause breaks in DNA strands b. Alkylation and cross linking DNA strands

c. Inhibition of DNA mediated RNA synthesis

d. Inhibition of polymerization of tubulin to form microtubules (d)

84. All of the following statements about vincristine are true EXCEPT a. It acts by inhibiting mitosis

b. Its prominent adverse effect is peripheral neuropathy c. It does not suppress bone marrow

d. It is a drug of choice for solid tumors (d)

(89)

85. All of following statements about are true about mercaptopurine EXCEPT a. It is metabolized by xanthine oxidase

b. It does not cause hyperuricemia

c. Its dose should be reduced when allopurinol is given concurrently d. It is an active metabolite of azathioprine

(b)

86. Which of the following immunosuppressants is not used for the treatment of cancers? a. Cyclophosphamide b. Cyclosporine c. Methotrexate d. 6-Mercaptopurine (b)

(90)

87. Which of the following drugs is not used in prostate carcinoma? a. Finasteride b. Diethylstilbesterol c. Testosterone d. Flutamide (c)

88. Pentostatin acts by inhibiting a. RNA dependent DNA polymerase b. Aldolase

c. Adenosine deaminase d. Adenylyl cyclase

(91)

89. Hand and foot syndrome is an adverse effect of a. 5-Fluorouracil b. Bleomycin c. Etoposide d. Actinomycin D (a)

90. Side effects of cisplatin include all of the following EXCEPT a. Nausea and vomiting

b. Nephrotoxicity c. Blindness

d. Ototoxicity (c)

(92)

91. Most common side effect of 5-fluoracil is a. G.I. toxicity

b. Bone marrow depression c. Cardiotoxicity d. Neurotoxicity (a) 92. Sterility is caused by a. Vinca alkaloids b. Alkylating agents c. Antimetabolites d.Actinomycin Ds (b)

(93)

99. Neoadjuvant chemotherapy is used in all except a. Esophageal carcinoma

b. Breast carcinoma c. Thyroid carcinoma

d. Non- small cell carcinoma of lung (c)

100.Which of the following anticancer drugs can cross blood brain barrier? a. Cisplatin

b. Nitrosourea c. Vincristine d. Vinblastine

(94)

101. Which of the following drugs produce significant nephrotoxicity? a. Cisplatin b. Carboplatin c. Vinblastine d. Vincristine (a)

102. Phocomelia is due to teratogenic effects of a. Thailidomide

b. Chlopromazine c. Methotrexate d. Carbamzepine

(95)

103. Folinic acid counteracts the toxicity of a. Doxorubicin b. Methotrexate c. Cyclophosphamide d. Fluorouracil (b)

104. Which of the following antineoplastic and immunosuppressant drugs is a dihydrofolate reductase inhibitor?

a. Methotrexate b. Adriamycin c. Vincristine

d. Cyclophosphamide (a)

(96)

105. Toxicity of nitrogen mustards can be decreased by a. Amifostine b. Folinic acid c. GM-CSF d. MESNA (c)

106. Which one of the following alkaloids is used as anticancer agent? a. Vincristine

b. Papaverine c. Ephedrine d. Atropine

(97)

107. The antimalignancy drug which is potentially cardiotoxic is a. Doxorubicin b. Bleomycin c. Fluorouracil d. Dacarbazine (a)

108. The drug of choice in choricarcinoma is a. Methotrexate

b. Actinomycin –D c. Vincristine

d. 6-thioguanine (a)

(98)

109. “Stocking and glove” neuropathy is seen in a. Vinblastine b. Paclitaxel c. Etoposide d. Mitroxantrone (b)

110Drug that is radioprotective is a. Paclitaxel

b. Vincristine c. Etoposide d. Amifostine

(99)

111. Hemorrhagic cystitis is caused by a. Cyclophosphamide b. Ifosfamide c. Vincristine d. Adriamycin (b)

112. Which of the following anti-cancer drug is cell cycle specific? a. Cyclophosphamide

b. Vincristine

c. Nitrogen mustard d. Doxourubicin

(100)

113. Which of the following anticancer drug is not ‘S’-phase specific? a. Methotrexate b. Meracaptopurine c. Ifosfamide d. Thiouanine (c)

114. All are alkylating agents except a. Cyclophosphamide

b. Lomustine c. Busulfan d. Zalcitabine

(101)

115. Chemotherapy is not useful in a. Chondrosarcoma b. Wilm’s tumor c. Choriocarcinoma d. All (a)

116. Cisplatin does not cause a. Cardiomyopathy

b. Nephrotoxicity c. Neuropathy d. Tinnitus

(102)

117. Cyclophosphamide can cause a. Hemorrhagic cystitis b. Cardiomyopathy c. Neuropathy d. Convulsions (a)

118. Which of the following is not an early adverse effect of methotrexate? a. Hepatic fibrosis

b. Myelosupression c. Nausea

d. Stomatitis (a)

(103)

119. Which of the following is not an antineoplastic antibiotic? a. Actinomycin D b. Doxorubicin c. Bleomycin d. Spiramycin (d)

120. All cause myelosuppression except a. Docetaxel vincristine

b. Vincristine c. Methotrexate d. Irrnotecan

(104)

121. Leucovorin rescue is related to a. Methotrexate toxicity b. Cyclophosphamide toxicity c. Oncovin toxicity d. Cisplatin toxicity (a) 122. Which of the following causes peripheral neuritis?

a. Methotrexete b. Vincristine c. Busulfan

d. Cyclophosphamide

(105)

123. The drug of choice for chronic myeloid leukemia, is a. Chlorambucil b. Busulfan c. Vincristine d. Procarbazine (b)

124. Proliferation independent agents include all the following except a. Vincristine

b. Carmustine c. Melphalan

d. Cyclophosphamide

(106)

125. People with high risk for development of breast cancer should be treated by prophylactic admini-stration of a. Tamoxifen b. Aminoglutethimide c. Diethyistibesterol d. Flutamide (a)

126. Which of the following is widely used in the management of carcinoma breast? a. Actinomycin D

b. Bleomycin c. Doxorubicin d. Dacarbazine

(107)

127. Rituximab is used in a. Hodgkin,s disease

b. Acute myeloid leukemia c. Non-Hodgkin lymphoma d. Multiple myeloma

(c)

128. Allopurinol potentiates action of a. Azathioprine

b. Busulfan c. Actinomycin d. Procarbazine

(108)

129. Alkylating agengts include

a. Doxorubicin

b. Cholorambucil

c. Vinblastine

d. Busulfan

e. Methotrexate

(b, d)

(109)

69. Sterile hemorrhagic cystitis is caused by a. Busulfan b. Ketoprofen c. Methicillin d. Cyclophosphamide (d)

70. A 50 year old woman, Hema has been diagnosed with locally advanced breast cancer and recommended for chemotherapy. She has five years history of myocardial infarction and congestive heart failure. Which antineoplastic drug should be best avoided?

a. Anthracycline b. Alkylating agent c. Platinum compound d. Bisphosphonates

(110)

67. Which of the following statements is FALSE regarding vincristine? a. It is an alkaloid

b. Its use is associated with neurotoxicity c. It does not cause alopecia

d. It is a useful drug for induction of remission in acute lymphoblastic leukemia (c)

68. A patient with cancer developed extreme degree of radiation toxicity. Further history revealed that the dose adjustment of a particular drug was missed during the course of radiotherapy. Which of the following drugs required a dose adjustment during

radiotherapy in order to prevent radiation toxicity? a. Vincristine

b. Dactinomycin

c. Cyclophosphamide d. 6- Mercaptopurine

(111)

37. Ifosfamide belongs to which group of anticancer drugs? a. Alkylating agents b. Antimetabolites c. Mitotic inhibitors d. Topoisomerase inhibitors (a)

38. A 56 year old female presented with breast carcinoma and she was prescribed herceptin (trastuzumab). Which of the following statements regarding this drug is true?

a. It is an antibody produced entirely from mouse con taining no human component. b. It is a monoclonal antibody produced by injecting her-2 antigen

c. It is a polyclonal antibody

d. It is a monoclonal antibody containing only human component (b)

(112)

93. Which of the following is a common side effect of cisplatin a. Diarrhea b. Vomiting c. Pulmonary fibrosis d. Alopecia (b)

94. The antimetabolite ‘X’ inhibits DNA polymerse and is one of the most active drugs in the treatment of leukemia. Although myelo-suppression is done limiting, the drug may also cause cerebellar dysfunction, including ataxia and dysarthria. Which of the following can be ‘X’? a. Bleomycin b. Cytarabine c. Mercaptopurine d. Methotrexate (b)

(113)

95. Which of the following antineoplastic drugs should not be administered to a chronic alcoholic patient due to risk of development of disulfiram like reaction?

a. Dacarbazine b. Procarbazine c. Melphalan d. Hydroxyurea

(b)

96 Roopa devi, a 65 year old female with overian cancer is being treated with cisplatin based chemotherapy. All of the following are used to limit the toxicity of cisplatin except

a. N-acetylcysteine b. Slow rate of infusion c. Chloride dieresis d. Amifostine

(114)

97. Roopmati, A 56 year old femal with lymph node positive breast cancer was treated with systemic chemotherapy. Four weeks later, she developed frequent urination, suprapublic pain, dysuria and hematuria. Which of the following could have prevented this patient’s condition?

a. Folinic acid b. Mesna c. Dexazoxane d. Amifostine

(b)

98. Sunder, a ypung male was diagnosed as suffering from acute myeloid leukemia. He was started on induction chemotherapy with doxorubicin based regiments. Induction regimen was successful. Two months later, he presents to opd with swelling of both the feet and breathlessness on climbing the stairs. He also complains the he had to wake up many times because of breathlessness. Which of the following is most likely responsible for this patient’s symptoms?

a. Restrictive cardiomyopathy b. Hypertrophic cardiomyopathy c. Dilated cardiomyopathy

d. Pericardial fibrosis (c)

(115)

• It is important to remember that antimetabolites such as

cytarabine, 5-FU do not cause acute toxicity

• Most of the hormones and hormone antagonist do not

cause acute toxicity

• All alkylating agents such as chlorambucil

cyclophosphamide, melphalan etc cause nausea and

vomiting as acute toxicity.

• Most of the natural products such bleomycin vincristine

and vinbalstine etc used for cancer chrmotherapy cause

nausea and vomiting as acute toxicity

(116)

Substances used to reduce the toxicity of anticancer drugs

1. Leukovorin/citrovorum factor folinic acid

2. Xanthine oxidase inhibitor allopurinol

3. Colony stimulating factor for Neutrophils filgrastim &

sargramostim, For RBCs darbopoetin –α &

erythropoietin.

4. Thiophosphate cytoprotectants amifostine

5. Acrolein conjugator, mesna, acetylcysteine

6. Iron chelator, Dexrazoxane

(117)

References

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