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Expression, Characterization and Metallation Studies of Human Metallothionein Isoform 2a Using Electrospray Ionization Mass Spectrometry

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Figure

Figure 1. Cysteine alignments of human MT isoform sequences. Both the β  figure is modified from Zalewska different amino acid residues between MT1a and MT2a are highlighted in red
Figure 2. Molecular model structures of human metallothionein 1a. Left side shows the domain and right side shows the cluster formation of MT (one cysteine (yellow) bound to more than one Cd (green) formed clusters)
Figure 3. The sequence of human metallothionein 2a with S-tag (blue). Seven divalent metals (green) bind to 20 cysteines (yellow)
Figure 4. Metal-dependent protein folding of metallothioneins binding up to four Cd, then the consists of four Cd and the in vitro
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