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Discovery of the congenital nephrotic syndrome gene discloses the structure of the mysterious molecular sieve of the kidney

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Figure

Fig. 1. Positional cloning of the congenital nephrotic syndrome gene.genes and APLP1 located in the 150 kb region were analyzed for mutations(E)  (B) markers D19S208 and D19S224, in a 920 kb region containing a few knownwhich overlapping cosmid clones were
Fig. 2. Expression of nephrin mRNA in human embryonic kidney by in situ hybridization.
Fig. 3. Schematic domain structure of nephrin. The red box representsthe transmembrane domain
Fig. 5. Immunoelectron microscopic localization of nephrin in humanrenal glomeruli.ity purified IgG against the extracellular region of recombinant humannephrin and 10 nm gold -coupled secondary antibody
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