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CRISPR/Cas9 mediated genome editing induces exon skipping by alternative splicing or exon deletion

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Figure

Fig. 1 sgRNA targeting Kras induces exon skipping in single cell clones.Thechain reaction (RT-PCR) primers
Table 1 Genomic lesion and mRNA splicing results of single cell clones
Fig. 2 Ctnnb1were designed to target exon 3: strong sgRNAs inphosphorylation amino acids 33, 37, 41, and 45 promotes degradation of the sgRNAs targeting exon 3 induces exon skipping
Fig. 3 Cas9 nuclease activity required for skipping of one or more exons. a RT-PCR analysis of Ctnnb1 mRNA in KP cells transduced with lentivirusesthat encode sgCtnnb1.2 and nuclease-defective Cas9 (dCas9), dCas9-KRAB fusion, or WT Cas9
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