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The ubiquitin ligase Siah is a novel regulator of Zeb1 in breast cancer

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Figure

Figure 1: Siah protein levels decrease during EMT. (A) Protein expression levels, as assessed by Western blot, of E-cadherin in MCF-7 untreated control cells and cells undergoing EMT, as induced by a combination of TGF-β and TNF-α treatment
Figure 2: Siah knockdown induces an EMT gene profile. (A) The efficiency of Siah knockdown with Dharmacon SMARTpool siRNAs as confirmed by qPCR
Figure 3: Siah causes reduction of Zeb1 protein levels. (A) Western blot of Zeb1 protein abundance in U2OS cells expressing Zeb1 alongside increasing concentrations of Siah1 or Siah2 (50 ng, 250 ng, 500 ng) as indicated
Figure 4: PyMT-Siah2-/- cell lines undergo spontaneous EMT. (A) Phase contrast images of PyMT-WT and PyMT-Siah2-/- cells in 2D culture
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