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Preservation of mouse ovarian tissue follicle morphology and ultra-structure after vitrifying in biotechnological protocols

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Figure

Table 1 Number and percentage of intact (Int) and degenerated (Deg) follicles of various stages isolated from vitrifiedand non-vitrified ovarian tissue after Trypan blue staining
Figure 1 Morphological images of the mouse ovarian tissue. A)vitrified in 5% EG + 5% DMSO,vitrified alone in 10% EG, non-frozen, B) CV1 group that vitrified alone in 5% EG, C) CV2 group that D) CV3 group that vitrified alone in 5% DMSO, E) CV4 group that vitrified alone in 10% DMSO, G) CV5 group that H) CV6 group that vitrified in 10% EG + 10% DMSO and I) CV7 group that equilibrated in CV5 and then CV6 andfinally vitrified in CV6 concentration.
Figure 4 The electron micrograph of granulosa cells of a secondary follicle. ATEM: ×4646,ovaries in double cryoprotectant, showing arrangement of mithochondria m, numerous lipid droplets Lp, vacuole V and atypical or irregularlyshaped mithochondria with lo
Figure 5 Transmission electron microscopy images of the secondary follicle from single cryoprotectants

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