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Systematic comparison of single-chain Fv antibody-fusion toxin constructs containing Pseudomonas Exotoxin A or saporin produced in different microbial expression systems

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Academic year: 2020

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Figure 1 Expression & characterization of the 4KB scFv.scFv (continuous line) or 10Red lines indicate the MFI obtained by staining Daudi cells with the scFv (continuous line) and mAb (dashed line) previously incubated at37°C for the same time lengths as fo
Figure 2 Constructs for the expression of toxin-based fusions in E. coli. Schematic representation of 4KBscFv (A), PE (B and C) and saporin(D)-derived constructs
Figure 3 Characterization of recombinant ITs expressed inof 4KB-PE40 (blue diamonds), 4KB(218)-PE40 (green circles) and 4KB(218)-SAP (red triangles) analyzed by flow-cytometry using Daudi cells incubated at E
Figure 6A) fusing the sequences coding for the anti-With this aim, we prepared a panel of constructs (see,CD22 VH and VL domains alternatively connected byusing (G4S)3 or 218 linkers, as described for 4KB-PE40,to a saporin yeast-optimized sequence [30] either carry-ing an N- or C-terminal hexahistidine tag.
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