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Accurate and efficient amino acid analysis for protein quantification using hydrophilic interaction chromatography coupled tandem mass spectrometry

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Figure

Fig. 1 a Chromatographic separation of twenty amino acids using a Z-HILIC column b. Leucine and isoleucine were separated capitalizing on the HILIC-water layer
Table 1 Performance characteristics of 20 amino acids for quantitative analysis
Table 2 Quantitation of  ubiquitin and  BSA standards as  determined by  LC–MS/MS isotope dilution and  AccQ-Tag methods
Fig. 2 Quantitation of individual amino acids upon hydrolysis using three different methods
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