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RavN is a member of a previously unrecognized group of Legionella pneumophila E3 ubiquitin ligases.

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Figure

Fig 1. RavN has ubiquitin ligase activity. (A) The silver-stained SDS-PAGE gel showing proteins present on either uncoated control beads (left) or His6-RavN-coated beads before (right) or after (center) incubation with lysate from HEK293T cells
Fig 2. The N-terminal region of RavN mediates E3 ligase activity. (A) Shown here is the schematic representation of the RavN fragments examined in theubiquitylation assay in (B)
Table 1. Crystallographic data collection, phasing and refinement statistics.
Fig 4. The U-box-E2 binding interface is conserved in RavN. (A) Superimposition of RavN1-55 (orange) and two related E3s (RING domain of Ark2C, light green;PDB 5D0K; U-box domain of CHIP, dark pink, PDB 2OXQ) in complex with their respective E2s (UbcH5b an
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