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Prevention of C5aR1 signaling delays microglial inflammatory polarization, favors clearance pathways and suppresses cognitive loss

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Figure

Fig. 1 Performance in Novel Object and Object Location Memory Tasks. Animals were tested in novel object recognition test (with a novel object (solid square) (object location memory test (for iNOR (a,b) and novelc-e) after 5 days of handling and 4 days of
Fig. 2 Fibrillar plaque and CD45 microglial expression in hippocampus. Representative pictures of thioflavine-S staining (fibrillar plaques) (toppanels) and CD45 staining (microglia) (lower panels) at 7 (a) and 10 months (c)
Fig. 3 Glia clustering around plaques are CX3CR1+CCR2−. Brain sections of (a) WT, (b) Arctic, (c) C5aR1KO, and (d) Arctic/C5aR1KO mice(all CX3CR1+/GFPCCR2+/RFP) at 2, 5, 7, and 10 months were fluorescently labelled in blue with anti-Aß antibody (1536)
Fig. 4 Temporal gene expression profiles across four genotypes. (a) RNA-seq experiments were performed using duplicate samples from WT (blue),C5aR1KO (red), Arctic (green) and Arctic/C5aR1KO (orange) mice at 2, 5, 7 and 10–11 months
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