www.scanburresearch.com
ContaCt us
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Customer serviCe
Laboratory test management (Ltm™) Help Desk
email: Ltm@crl.comLaboratory testing management (Ltm™) is Charles river’s online, interactive order entry and results management system that centralises your health and genetic testing programmes into one virtual location. use Ltm™ to search for tests and sample submission information, create and track orders, as well as archive and trend your testing results. to learn more, visit www.criver.com/ltm
the Ltm™ Help Desk can assist you with: • Online ordering and sample shipping • Results retrieval and interpretation • Account management and troubleshooting • One-on-one or group demonstrations and training • Creating custom templates and reports
• Summarising and trending data
www.scanburresearch.com
We speak Danish, swedish, norwegian, Finnish, english and German.
Denmark & southern sweden (malmö and Lund)
tel. +45 5686 5644 @ orders-dk@scanbur.eusweden
tel. +46 8 594 767 80 @ orders-se@scanbur.eunorway
tel. +45 5686 56 48 @ orders-no@scanbur.euFinland & the Baltic states
tel . +358 40 125 2694@ orders-fin@scanbur.eu
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orDerinG inFormation
orDerinG inFormation
animals, products and services can be ordered from our Customer service Department:
Cancellation Policy
Guinea-pigs:Cancellation accepted before the guinea-pigs are weaned (2 weeks). rabbits:
time mated females
• 4 weeks before mating date, we invoice the price of an animal of 14 weeks old.
• 2 weeks before mating date, we invoice the price of an animal of 16 weeks old.
• 1 week before mating date, we invoice the price of an animal of 18 weeks old.
• 1 day before mating date, we invoice the price of a time mated female.
other rabbits:
Cancellation accepted before they are 10 weeks old.
rodents:
• Standard outbred/inbred strain and standard age/weight range (catalogue strain < 8 weeks old): no cancellation fee before animals are prepared for dispatch.
• Standing orders: 6 weeks notice for cancellation/modification • Specific strains and special age/weight range: no cancellation
allowed, “take or pay”
• Mated/pregnant females (standard strains OFA, CD, WISTAR, oF1, and CD1): no cancellation fee if the order is cancelled before mating. after mating, invoice at the price list cost.
• Other strains / specific orders (large quantities, age range…): invoice of cost up to the date of cancellation.
Additional Charges - Specific Orders
Research models are sold within specific weight ranges or at a certain age. For specific orders outside the standard weight range or age, additional charges may apply.
Denmark & southern sweden (malmö and Lund)
tel. +45 5686 5644 @ orders-dk@scanbur.eusweden
tel. +46 8 594 767 80 @ orders-se@scanbur.eunorway
tel. +45 5686 56 48 @ orders-no@scanbur.euFinland & the Baltic states
tel . +358 40 125 2694Preconditioning Service S, Surger y and contract Service S
Preconditioning
ServiceS, Surgery
and contract
ServiceS
Our services can help alleviate the space, time and labour costs involved with refining a model
to meet your unique research requirements. Whether you are looking for animals fed a special
diet, or reared to a certain age, or sugically modified, Charles River has state-of-the-art animal
facilities, professional animal care and robust model selection to deliver study-ready animals
right to your door.
Contact us with your enquiry, please refer to page 6.
Preconditioning Services
Preconditioning Services (page 45)
Charles River offers a flexible range of
preconditioning services including model aging programmes and diet inductions. During the preconditioning period, various phenotypic
parameters can be measured and recorded on an
as-needed basis. In addition, surgical manipulation can be performed on the animals as part of the preconditioning process.
Surgical Services (pages 45-47)
Charles River offers a variety of surgical services, including vascular and non vascular catheterisations; device implants; and soft tissue, neurological and cardiovascular procedures, on various species - rat, mouse, Guinea pig - in its European dedicated production facilities. Charles River’s surgical procedures are developed
in-house and subject to review and approval by their
Ethics’ committee.
Charles River’s biosecurity measures include barrier-type surgical suites, use of dedicated laminar flow workstations by each surgeon and housing of animals in adapted caging systems during the animals’ stay in surgery.
Surgical Support
• The “Rent-a-Surgeon” programme allows one of Charles River’s surgical technicians or veterinarians to travel to your facility for an entire day or more to perform surgical procedures as requested by you. Please note that the “Rent-a-Surgeon” service is also available for large animal surgeries.
• The “Rent-a-Trainer” programme provides a full day or more of on-site surgical training on approved procedures for your staff.
Charles River Surgery Experts can also help you develop new surgically prepared models.
Biospecimens (page 48)
Charles River can provide rat, mouse, guinea pig or gerbil biospecimens (tissues, organs,
blood products) upon request. Biospecimens
are collected and processed in Charles River’s dedicated facilities by experienced technicians.
antibody Production Protocols
(page 49)
Charles River serves as a flexible and reactive partner for conducting your immunisation protocols on a wide range of species from SPF rodents to large animals.
aLZet
®osmotic Pumps
(pages 50)
ALZET® osmotic pumps are miniature infusion systems that deliver test agents at controlled
rates into mice, rats and other laboratory animals.
SCANBUR Research is distributor for Denmark, Sweden, Norway, Finland and the Baltics.
Biological and Quality control
testing (pages 52-53)
Charles River offers a set of tests conducted in compliance with Good Manufacturing Practices in its Pharmaceutical Facility: Sample preparation, in vivo pyrogen testing, abnormal toxicity tests, safety tests, systemic injection tests, biological activity testing … Preconditioning Service S, Surger y and contract Service S
2014 | www.scanburresearch.com
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Preconditioning Services
Charles River Preconditioning Services allow researchers to save valuable resources by purchasing
animals ready to use for your research. Charles River offers preconditioning studies from standardised
to highly individualised protocols to suit your requirements. For further information, please contact us
(see page 6)
.
PReConditioning SeRviCeS
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Feeding Studies / Surgical and Chemical
Manipulations
Charles River offers customised preconditioned models in accordance with the objectives/requirements of your experiments.
Models can be pre-fed with special diets to induce obesity,
hypertension, stroke or other conditions. our team can advise you on the selection of the appropriate diet depending on the model. Charles River offers specific disease induction by injection or irradiation (e.g. diabetes induced by streptozotocin injection, pristanisation…).
Physiological modifications or specific diseases can be induced by surgical manipulation upon request, see page 48.
Aging Studies
in some models, disease conditions develop only as the animal ages. Charles River can hold and care for the animals in a barriered preconditioning room and deliver them to you as needed.
in addition, Charles River also offer ‘off the shelf’ aged models. Please enquire with Customer Services for availability.
Aged Animals Age
JAX™ Mice strain C57BL/6J Up to 15 months
Crl:WI(Han) Enquire
Crl:OFA(SD) Enquire
therapeutic Field induced Model Surgically induced Model Contract Research Service
Metabolic and Cardiovascular Type 1 or 2 Diabetes Left Coronary Artery Ligation Custom Diet Administration
(Rat / Mouse) (Myocardial infarction) • High Fat / Energy Diet Diet Induced Obesity Models Ischemia/perfusion • Cholesterol Diet • C57BL/6 DIO Mouse • Cafeteria Diet • CD DIO Rat Organ / Tissue Collection • Obese Prone Rat
STZ Injection
Nephropathy and Diet Induced Models Renal Failure Model Custom Diet Administration
Hypertension • Dahl/Salt Sensitive Rat (Unilateral or 5/6 Nephrectomy) • High Salt Diet • Stroke Prone Rat Ischemia/perfusion Organ/Tissue Collection Gerontology and Aged Animals Parkinson’s Model Custom Ageing
Neurodegeneration (6-OH Induction) Organ / Tissue Collection
ALZET® osmotic pump Histopathology
implantation
Brain canulation
Endocrinology Aged Animals Ovariectomy Custom Ageing
Hysterectomy / Castration Custom Diet Administration
ALZET® osmotic Pump Compound Administration
Implantation - Pellets
ADME & PK Vascular Catheterizations Compound Administration Non Vascular Catheterizations Intermediate / End Point Blood
Sampling
Clinical Observation
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Surgery Services
Charles River offers a variety of surgical services on three species - rats, mice and guinea pigs - at their
various locations across europe. their surgical procedures are developed in-house and are subject to
approval by their ethics committee. the procedures are performed by trained surgical technicians under
the supervision of veterinary staff with regular quality control checks.
vascular Catheters
(1)Weight Rats € Mice € guinea Pigs €
Carotid artery > 25 g mice 110,35 138,55 -
> 150 g rat
Femoral artery > 200 g 120,77 -
Jugular vein > 25 g mice 97,10 141,20 141,20
Jugular vein connected to harness > 100 g rat 157,10 171,20 201,20
Femoral vein > 100 g 123,10 -
Portal vein (through mesenteric vein) > 250 g 186,05 Enquire
Double jugular vein > 150 g 157,65 -
Carotid artery and jugular vein > 150 g 209,25 -
Femoral artery and femoral vein > 200 g 239,15 -
Jugular vein and femoral vein > 100 g 216,35 -
Portal vein and jugular vein > 250 g 278,56 -
Femoral vein connected to harness > 100 g 186,60 -
Jugular vein and femoral vein connected to a double harness > 100 g 314,60 -
-(1) Prices given are for standard polyurethane catheters. other types of catheter are available on request. Contact us for additional information.
non-vascular Catheters
(1)Weight Rats € Mice € guinea Pigs €
Bile duct (connected to the duodenum) > 250 g 226,05 -
Bile duct connected to a double harness > 250 g 371,90
Duodenal catheter* > 150 g 175,60 -
Stomach catheter* > 150 g 150,10 -
-(1) Prices for standard Polyurethane catheters. other types of catheters are available on request, contact us for additional information. * infusion only.
excision Surgery
Age/ Weight Rats € Mice € guinea Pigs €
Hypophysectomy* > 4 weeks 42,95 - - Orchidectomy (castration) > 3 weeks 24,05 24,05 24,05 Ovariectomy > 3 weeks 34,85 34,85 34,85 Splenectomy 5 - 6 weeks 47,25 47,25 Adrenalectomy 5 - 6 weeks 47,25 47,25 Thyro-parathyroidectomy 5 - 6 weeks 58,35 - Thymectomy > 200 g 166,65 - Vasectomy > 3 weeks 41,40 41,40
Thyroidectomy with parathyroid implant 5 - 6 weeks 66,90 - - Parathyroidectomy 5 - 6 weeks 66,90 - - Partial hepatectomy (70%) 8 weeks 136,10 - -
* Price for oFA Rats. other strains: please consult us.
disease models
Weight Rats € Mice € guinea Pigs €
Myocardial infarction (left coronary artery ligation)* > 28 g 340,50 340,50 -
Unilateral nephrectomy > 45-50 g 48,15 48,15 48,15
Nephrectomy 5/6 > 150 g 108,35 - -
Nephrectomy 2/6 > 150 g 48,10 -
Parkinson’s disease (chemical induction) > 225 g 250,95 -
Arthritis (Freund Adjuvant)* > 140 g 42,20 -
Arthritis (Monoiodoacetate Sodium)* > 125 g 47,85 -
-* Performed only on specific strains. Contact us.
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neurological Procedures
Weight Rats € Mice € guinea Pigs €
Intralateral brain cannulation (1) > 175 g 208,60 237,30 -
Double intralateral ventricle cannulation (1) > 175 g 219,65 - -
3rd ventricle cannulation > 175 g 208,60 - -
Unilateral ventricle cannulation (1) > 175 g 219,65 237,30 -
Bilateral subdiaphragmatic vagotomy > 175 g 129,00 - -
Intrathecal Catheter (1) > 175 g 205,40 - -
Intracisternal Catheter > 175 g 205,40 -
-(1) For infusion only; no sample collection.
device implantations
(1)Age/Weight Rats € Mice € guinea Pigs €
ALZET® osmotic pumps
- Connected to a catheter > 3 weeks 47,10 47,10 - Connected to a BIK > 3 weeks 60,30 60,30 - Subcutaneous > 3 weeks 44,40 44,40 - Intraperitoneal > 3 weeks 59,75 59,75
Electrocardiogram sensor (ECG) > 225 g 138,60 Enquire
Electroencephalogram sensor (EEG) > 200 g 151,65 -
Electromyogram sensor (EMG) > 200 g 119,25 -
Blood pressure sensor (BP) > 200 g 172,80 - Blood pressure + Electrocardiogram sensor (BP + ECG) > 200 g 204,90 -
Temperature sensor > 200 g 119,25 119,25
Electromyogram + Electroencephalogram sensor (EMG + EEG) > 200 g 270,65 -
-(1) Price of implants is not included in the price of the surgical procedure. telemetry implants are to be provided by the customer.
Cancellation policy: Cancellations are accepted until 10 days before the scheduled date of surgery. no cancellations are accepted after this date.
SuRgeRy SeRviCeS
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training
• Customized Training Sessions : Charles River offers specific training programs that meet exactly your requirements and aim to provide you with the good practices of surgery and peri operative care technics. topics include: anesthesia, analgesia, asepsis, peri operative care, surgical procedures. Contact us (see page 6).
• Charles River and Professor René Rémie are combining their expertise
to offer surgery training courses combining lectures with “hands-on” exercises to ensure that you receive the most comprehensive training experience. Please contact us to learn more about future sessions (see page 6).
Customised Surgical Procedures
Many of our specialised surgical offerings were developed based on customer demand. if you are interested in a surgically altered model that is not listed on the previous pages, please contact technical assistance to discuss the development of a customised procedure. Please consult us (see page 6).
on-site Surgery
Charles River offers surgical procedures performed by our surgeons at your site. Quotations are established individually for each project depending on the complexity and the number of procedures to be performed. Please consult us (see page 6).
From Surgery to Protocol
Surgically prepared animals are available as « ready-to-use models » for your research protocols. Charles River can also manage many aspects of your in vivo protocols, by:
• compound administration
• collecting blood for your pharmacokinetic studies • collecting many body fluids such as CSF, urine,…
• performing specific clinical observations and organ collection. Please contact us (see page 6)to discuss your project and see how we can help you design and perform your protocols.
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BioSPeCiMenS
tissues and organs
Charles River is able to supply normal tissues and organs from various species. Specimens are shipped in insulated dry ice igloos.
RAtS, MiCe, guineA PigS, RABBitS Adrenal – vessels - Bladder - Brain – Cerebrospinal Fluid - Colon - eyes - Jejunum - Heart - ileum - Kidney - Liver - Lung -
Milk (per ml) - oesophagus - Pancreas - Pituitary - Prostate - Salivary gland Stomach Skin (shaved) Spinal Cord
-Spleen - tails - testis – thymus - thyroid - urine (per ml) - uterus.
Please enquire for availability of other biospecimens
Blood Products
Blood, serum, and plasma can be collected from various species, including rodents, rabbits, hen and turkey.
Collection of Sera and Plasma
Sera will be collected from coagulated blood. After centrifugation sera are placed in different sized tubes or vessels. the plasma and blood is collected in monovettes that include an anticoagulant.
Standard anticoagulants are :
• Lithium-heparin (15 IU * heparin / ml blood) and sodium-heparin or • Potassium-EDTA K3 or K2 (1.6 mg/ml blood)
other anticoagulants, as :
• Sodium citrate solution (0,5 ml citrate solution per 5 ml blood). Citrate solution: 3.13 % sodium citrate dihydrate = 0.106 ml/l • Alsever’s solution 25/50%
are also available upon request.
transport of Blood Products and Biospecimens
Serum and plasma are stored at -20°C and usually transported on dry ice.
Blood, serum, and plasma will be transported by our own delivery trucks or a courier express service. transportation charges are calculated according to the destination and the means of transport used.
Packaging Price - €
Polystyren container (isotherm) 21,70
Insulated, cooled (+4°) 25,80
Insulated, dry ice 85,00
Biospecimens
Mice and Rats
description Amount in ml outbred Mouse inbred Mouse outbred Rat inbred Rat
oF1, nMRi and C57BL/6 and oFA, Wistar Han Fischer Rats
Cd1 mice BALB/c mice and Cd Rats
Price - €/tube Price - €/tube Price - €/tube Price - €/tube
Blood 1 Enquire Enquire Enquire Enquire
5 34,35 Enquire 19,60 46,40 10 67,35 Enquire 38,45 90,90 Plasma 1 12,70 Enquire 6,60 17,45 5 60,55 Enquire 31,00 Enquire 10 122,70 Enquire 60,65 Enquire Serum 1 12,70 Enquire 6,60 17,45 5 60,55 Enquire 31,00 Enquire 10 122,70 Enquire 60,65 Enquire
other volumes (ml) available on request.
gerbils, Hen, turkey, guinea Pigs and Rabbits
description Amount gerbils Amount Hen* Amount turkey* Amount guinea Pigs Amount Rabbits* in ml Price - €/ml in ml Price - €/tube in ml Price - €/tube in ml Price - €/tube in ml Price - €/tube
Blood 1-500 23,62 2 16,45 1 3,80 1 On request 501-1000 On request 5 41,05 5 44,15 5 17,30 10 19,30 10 78,25 10 84,10 10 33,90 20 37,80 Plasma 1-500 26,72 1 6,05 1 On request 501-1000 26,11 5 27,50 10 44,00 >1001 On request 10 54,15 20 On request Serum 1-500 25,94 10 96,35 1 6,05 1 On request 501-1000 25,36 5 27,50 10 44,00 >1001 On request 10 54,15 20 On request
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AntiBody PRoduCtion PRotoCoLS
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Antibody Production Protocols
With Charles River’s dual experience in breeding and service provision, they are a flexible and reactive
partner for conducting your immunisation protocols, from research quantity supplies to industrial
production on a wide range of species from SPF rodents to large animals (on request, after feasibility
study). the immunisation and sampling schedule is adapted to customer specifications. in accordance
with Charles River’s policy, a scientific justification and an ethical review are required prior to conducting
any protocols. Monoclonal and Polyclonal Antibody Production Services are developed and performed
in collaboration with Squarix Biotechnology, gmbH.
Polyclonal Antibody Production
Price (>3 animals)*
Immunisation according to our standard protocol
•Rabbit 417,00 (1 Rabbit)
•Guinea pig 247,00 (1 Guinea pig)
I. Complete Package One Price - €
•Peptide synthesis (purity >80%, 5-10mg, up to 15 AA included) •Support in selecting the peptide
•Conjugation to KLH, BSA or OVA (2 mg peptide, 20 mg KLH) Upon Request •Two rabbits, standard protocol
•One pre-immune serum, two serum samples, final bleeding
II. Complete Package Two
Complete package one, plus:
•3x ELISA antibody titre control Upon Request
III.Complete package Three
•Complete package two, plus:
•Preparation of a HYDRA-antigen affinity column (1 ml matrix)
•Affinity isolation of antigen specific antibodies out of 50 ml serum or cell culture supernatant
•Sequential elution of up to 2 fractions of different affinity Upon Request •Purification control of monospecific antibodies
•ELISA quality control
•Detailed documentation, chromatograms
Monoclonal Antibody Production
in vitro production of hybridomas
Basic Package Price - €
•Immunisation of three BALB/c mice, four injections within 3 months •Serum titer control by ELISA tests
• Spleen cell preparation, fusion with mouse myeloma cell line,
distribution of hybridoma cells onto 96 well plates, selection of hybridomas, ten plates in total Upon Request
•Detection of specific antibody in culture medium of 96 supernatants by ELISA with one antigen •Delivery up to 20 supernatants with the strongest immune reactivity
•Delivery up to 5 vital or cryopreserved positive clones
Standard Package Price - €
•Immunisation of three BALB/c mice, four injections within three months •Serum titer control by ELISA tests
•Spleen cell preparation, fusion with mouse myeloma cell line, distribution of hybridoma cells onto 96 well plates, selection of hybridomas, 20 plates in total
•Detection of specific antibody in culture medium of 96 supernatants by ELISA with one antigen Upon Request
•Delivery up to 30 supernatants with the strongest immune reactivity •Delivery up to 10 vital or cryopreserved positive clones
Additional antibody services are available including antigen synthesis as well as purification and labelling of antibodies.
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ALzet
®oSMotiC PuMPS
ALzet
®osmotic Pumps
ALzet
®
osmotic Pumps
SCAnBuR Research is ALzet® distributor in norway, Sweden, denmark, Finland and the Baltic States. ALzet® osmotic Pumps are miniature infusion systems that deliver test agents at controlled rates into mice, rats, and other laboratory animals. in addition to systemic administration, targeted delivery to an area remote from the site of implantation can be achieved by attaching a catheter to the pumps. ALzet® osmotic Pumps offer a good alternative to repeated animal dosing by injection or oral gavage. their continuous delivery eliminates peak and trough fluctuations and allows the effects of test agents to develop fully and reproducibly, particularly those with short half-lives. ALzet® osmotic Pumps help minimise animal stress that develops through frequent handling. they can eliminate the necessity for weekend and nighttime dosing. during infusion, no external connections are required, and the animals are untethered and unrestrained. Since ALzet® osmotic Pumps are fully implanted, they are tamperproof. individual caging of the animals is not required.
Comprehensive technical information is available online at www.alzet.com
Pump Model Specifications Product Code 1 pack of 10 - €*
volume duration Release Rate
1003D 100 µl 3 days 1,0 µl/hr 0000289 342,17 1007D 100 µl 1 week 0,5 µl/hr 0000290 402,69 1002 100 µl 2 weeks 0,25 µl/hr 0004317 494,69 1004 100 µl 4 weeks 0,11 µl/hr 0009922 589,92 2001D 200 µl 1 day 8,0 µl/hr 0000294 494,69 2001 200 µl 1 week 1,0 µl/hr 0000292 342,17 2002 200 µl 2 weeks 0,5 µl/hr 0000296 402,69 2004 200 µl 4 weeks 0,25 µl/hr 0000298 494,69 2006 200 µl 6 weeks 0,15 µl/hr 0007223 588,66 2ML1 2 ml 1 week 10,0 µl/hr 0000323 495,70 2ML2 2 ml 2 weeks 5,0 µl/hr 0000325 589,92 2ML4 2 ml 4 weeks 2,5 µl/hr 0000327 707,74
Each pack contains 10 mini-osmotic pumps, 10 flow moderators, 1 disposable filling tube and 1 instruction/specification sheet.
Accessories Product Code Price per pack of 10 - €
Filling Tubes - 100 µl pumps 0007988 32,68
Filling Tubes - 200 µl pumps 0007987 32,68
Filling Tubes - 2 ml pumps 0007986 32,68
PEEK Tubing - 100 µl pumps 0002612 22,60 PEEK Tubing - 200 µl pumps 0002496 22,60
PEEK Tubing - 2 ml pumps 0002511 22,60
Flow Moderators - 100 µl pumps 0002602 41,76
Flow Moderators - 200 µl pumps 0002486 41,76
Flow Moderators - 2 ml pumps 0002501 41,76
Coloured Flow Moderators - 100 µl pumps BLUE 0002609 60,00
Coloured Flow Moderators - 100 µl pumps TEAL 0002607 60,00
Coloured Flow Moderators - 200 µl pumps BLUE 0002489 60,00
Coloured Flow Moderators - 200 µl pumps TEAL 0002488 60,00
Coloured Flow Moderators - 2 ml pumps BLUE 0002504 60,00
Coloured Flow Moderators - 2 ml pumps TEAL 0002503 60,00
ALZAID compatibility kit 0004750 39,04
Brain infusion Kits (BiK) Product Code Price per pack of 10 - €
BIK 1 0004760 149,11
BIK 2 0008663 149,11
BIK 3 0008851 149,11
Cyanoacrylate Adhesive (loctite 454), pack of 5 x 3 g tubes 0008670 Enquire
Cannula Holder 1 0008860 330,00
Cannula Holder 2 0008861 480,00
• ALZET® Pumps are sold in units of 10 pumps, together with one filling tube. • The ALZAID® Test Kit contains enough material to test up to 4 substances.
• Brain Infusion Kit units contain material for 10 infusions: 10 infusion cannulas, 10 vinyl catheter tubes, 40 spacers, 1 instruction leaflet.
Flow Moderator test agent Semi permeable membrane osmotic layer impermeable reservoir
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ALzet
®oSMotiC PuMPS
51
Catheters Product Code Price each - €
Medical grade polyethylene (PE) and vinyl tubing - Sterile
PE60 (0.76 mm ID) - 15 cm length 0007750 38,70 Vinyl (0.69 mm ID) - 15 cm length 0007760 37,84 Unsterile per metre - connects to all ALZET flow moderators PE60 - 0.76 mm ID / PE50 - 0.58 mm ID Enquire
Special Catheters Product Code Price each - €
Rat Jugular Catheter 0007710 17,69
Rat Femoral Catheter 0007720 14,85
Rat Femoral Tapered Catheter - Custom order 0007730 Enquire
Rat Intrathecal Catheter 0007740 28,17
Rat Intrathecal Catheter - Short 0007741 28,17
Rat Intraperitoneal (IP) Catheter 0007770 19,30
Mouse Jugular Catheter 0007700 16,87
Mouse Jugular Catheter - Adjustable Length 0007701 17,52
Mouse Jugular Catheter - Large Tip 0007702 17,52
Mouse Intrathecal Catheter 0007743 26,99
Mouse Intraperitoneal (IP) Catheter 0007771 19,30
AutoClip® Wound Closure System (9 mm) Price - €
AutoClip® 100 clips 0009950 37,77
AutoClip® Kit 100 (includes Applier, Remover and 100 clips) 0009953 439,05 AutoClip® Kit 500 (includes Applier, Remover and 500 clips) 0009954 555,22
AutoClip® Applier 0009956 282,45
AutoClip® Remover 0009957 153,33
Reflex® Wound Closure System (7 mm) Price - €
Reflex 100 clips 0009971 68,80
Reflex Kit 100 (includes Applier, Remover and 100 clips) 0009972 556,83 Reflex Kit 500 (includes Applier, Remover and 500 clips) 0009973 887,70
Reflex Applier 0009974 282,45
Reflex Remover 0009976 225,96
Choose the best pump model for your study design
Presently, there are 12 pump models available that differ in volume (100 µl - 2 ml), duration (1 day - 6 weeks) and delivery rate (0,11 µl/h - 10 µl/h).dosage variability can be achieved by modifying agent concentration; duration can be extended by serial implantation of the pumps.
Bypass the Blood-Brain-Barrier
For direct delivery to the cerebral ventricles and brain tissue, the pumps can be combined with the Brain Infusion Kit 1, 2 or 3.Animal Considerations
Choose the right pump model to suit your animal and route of delivery. the table below lists the estimated minimum animal size required for implantation of ALzet® pumps.
Pump Model Mice Rats
Subcutaneous intraperitoneal Subcutaneous intraperitoneal 1003D 1007D 10 g 20 g 10 g 20 g 1002 1004 2001D 2001 2002 20 g N/A 20 g 150 g 2004 2006 2ML1 2ML2 N/A N/A 150 g 300 g 2ML4
Note: The minimum animal size estimates are based on experience with male Sprague Dawley rats and Swiss Webster mice. When using the pumps with other types or genders of rats and mice, or with animals other than rats and mice, these guidelines should be modified accordingly.
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ASSoCiAted SeRviCeS oveRvieW
Sample preparation
All types of preparation can be performed (raw materials and drug products or medical devices): Weighing, dilution, cold or hot extraction, autoclaving …
Price - €
Sample preparation Custom protocol Upon request
in vivo Pyrogen testing
tests are performed in a protected area including two housing rooms, one sample preparation room and one testing room. this is equipped with a fully integrated test management system, from temperature recording to interpretation of the results, in compliance with the european, uS and Japanese Pharmacopoeias (other Pharmacopoeias on request). tests are performed using rabbits that are bred in SPF units on the same site.
Price - €
in vivo Pyrogen test Pre-test and test on 3 rabbits Upon request
Abnormal toxicity test, Systemic injection test, Safety tests
our test unit includes housing rooms and a laboratory. tests are performed in compliance with the european, uS and Japanese Pharmacopoeais (other Pharmacopoeais on request) using SPF mice and guinea pigs sourced from Charles River barrier rooms.
Abnormal toxicity test, Systemic injection test, Safety tests Price - €
Mouse Custom protocol Upon request
Mouse + Guinea pig Custom protocol Upon request
Biological Activity testing
tests are conducted in our immunology and Cell Culture Laboratories. testing methods include: eLiSA, electro-immunodiffusion, and cell-based assays. For other methods, please contact us (see page 6).
Price - €
Biological activity testing Custom protocol Upon request
Cell culture and banking Custom protocol Upon request
other biological testing
All biological tests (e.g., biological reactivity test USP88, irritation and skin sensitivity assays ISO 10993-10, haemolysis test…) described in any Pharmacopoeia or Sponsor references can be performed. Species used are: rabbit, rat, mouse and guinea pig. Please contact us (see page 6) for further information.
Price - €
Biological testing Custom protocol Upon request
Pharmaceutical Quality Control (Biological tests)
Charles River offers a set of tests conducted in compliance with good Manufacturing Practices in our
Pharmaceutical Facility (authorised by the French Health Authorities in 2004). Furthermore, the Pyrogen
test activity is also cgMP compliant and registered with the Food and drug Administration (FdA) since
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ASSoCiAted SeRviCeS oveRvieW
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other Quality Control testing
endotoxin and Microbial detection: endosafe
®and Accugenix
®the endotoxin and Microbial detection division provides a comprehensive portfolio of QC testing solutions. A full line of rapid LAL testing systems, including the endosafe-PtS™ (portable handheld) , endosafe®-MCS™ (multi-cartridge) and our new endosafe-nexus™ (multi cartridges robotic system), facilitate reliable and accurate endotoxin testing with FdA-licensed LAL cartridges, providing quantitative test results in about 15 minutes. PtS™-based microbiological testing products are also available for glucan contamination and gram determination.
Charles River’s endotoxin testing solutions are complemented by Accugenix® services, with FdA-registered, cgMP-compliant contract microbial testing service laboratory. Accugenix® services are specialized in bacterial and fungal identification and strain typing of environmental isolates to meet internal quality standards and government regulations. With a recent partnership agreement Charles River endotoxin and Microbial detection division sell the Axcess™ System, to combine the precision and robustness of the Bruker MALdi Biotyper technology with the Accugenix® cgMP compliant microbial database and software, and maximize performance for environmental monitoring applications while simplifying customers’ validation commitment.
Bolstered by broad-based, accessible technical support, our progressive technologies and proprietary methods of endotoxin and microbial testing ensure the safe manufacturing and timely release of your product. Charles River’s european testing services has been relocated in a purposely designed larger facility in ecully, France. the new location of the facility will allow Charles River to answer the growing demand for LAL services and fully integrate its microbial identification activity.
For more information on those programmes, contact us (see page 6).
Mouse embryo Assay (MeA)
the mouse embryo assay (MeA) is currently the most widely used bioassay to test for toxicity and functionality of media and devices used in medically assisted procreation. The Charles River group utilizes fresh one- and two-cell embryos produced from B6D2F1 mice to perform this service. the embryos are collected and incubated in the culture media (or device) to be tested for a predetermined amount of time.
After exposure, the embryos are then cultured for approximately 72-96 hours in a defined and tested culture medium and scored for embryos development until blastocysts. Charles River requires an 80% development rate from its control and test article/medium for the result to be considered a passing score. exposure conditions to test media or material are defined in collaboration with the customer.
tests are conducted in the embryology laboratory based in the Charles River genetically engineered Models and Services facility.
Mouse embryo Assay (MeA) Price - €
MEA testing 25 embryos exposed to media or product and tested for blastocyst development Upon request
Resea Rch animal Diagnostic se Rvices
ReseaRch animal
Diagnostic
seRvices
Rather than just identifying the problem, the Charles River 360 Diagnostic Programme is the
only comprehensive partner from Prevention to Resolution. Through development of innovative
solutions like LTM™ and PRIA Panels, our goal is to help you manage your programme
effectively and efficiently. To learn more about the 360 Diagnostics Mission and the tools you
need, and to request free sample shipping materials for animal health surveillance, cell line
testing and environmental monitoring visit www.criver.com/dx
Research animal Diagnostic services
Charles River’s goal is to identify and incorporate the most analytically sensitive and specific technologies
available in order to provide the most accurate and reproducible results. They perform rigorous research and development prior to the introduction of any new assay or technology and they implement standard operating procedures to ensure that your samples yield the correct results. For details regarding the Charles River services and reagents, including testing platforms, technology and sample submission, please visit
www.criver.com or contact us. Please refer to page 6 for contact details.
Diagnostic laboratory locations
Charles River has multiple diagnostic laboratories to provide a local and convenient option. They receive live animals and samples for diagnostic testing at all their laboratories:
• North America (Massachusetts and California, US) • Europe (France)
• Asia (Japan)
The same techniques and technologies are implemented at all the diagnostic sites worldwide,
giving you confidence that Charles River will
provide the consistent results that you have come to expect.
complimentary shipping materials
Charles River provides clients with International
Air Transport Association (IATA)-compliant sample and animal shipping containers (sample shipping materials, shipping labels and containers, live-animal shippers, etc.). To receive materials, simply
contact us. Please refer to page 6 for contact details.
consultation and education
Since health concerns vary greatly with facility size
and research type, we can, in cooperation with Charles River’s Professional and Technical staff
members, provide one-on-one consultation and
educational presentations on topics, including and not limited to:
• Routine and quarantine health surveillance programme design or refinement, including FELASA recommendations
• Results interpretation
• Prevalent infectious agents, outbreak detection
and management
• Technical training for users of serology reagents
and LTM™
submission Forms
In order to expedite the submission process, ensure the safety of the Charles River staff, and adhere to regulations, we kindly remind you to
please sign the Health Hazards Declaration Form, which includes the presence of zoonotic or class 2 agents as mentioned on the Submission Form.
Research animal health
surveillance
• PRIA (PCR Rodent Infectious Agent) panels for
Mouse, Rat, Gerbil, Rabbit, Hamster, Guinea pig
• Health monitoring, serology, microbiology and
additional tests for : Mouse, Rat, Gerbil, Rabbit, Hamster, Guinea pig
• Ferret testing services • Simian testing services
• Serology reagents (MFIA®, ELISA and IFA) for
in-house surveillance
cell line and Research Biologics
screening
PCR Panels to screen for:
• Rodent infectious agents • Human infectious agents
Cell Line Examination and Report (CLEAR) Panel
to detect cell line contamination
environmental monitoring
• Microbial/bioburden testing of water, feed and
bedding
• Surface testing
health Data management
• Laboratory Test Management (LTM™)
LTM™ is Charles River’s online, interactive order entry and results management system that centralises your health and genetic testing programmes into one virtual location.
Use LTM™ to search for tests and sample
submission information, create and track orders, as well as archive and trend your testing results. To learn more, visit www.criver.com/ltm
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Rodent and Rabbit Health Surveillance
PRIA (PCR Rodent Infectious Agent) Panels
Direct Animal Sampling
detect viruses, bacteria and parasites in principal animal by screening non-invasive samples with a PRia Panel. Up to 10 samples (e.g. one fecal pellet each from 10 animals) can be combined and submitted as one pool for PcR testing at no additional charge.
Panel Mouse Rat Hamster Guinea Pig Rabbit Gerbil
Enteric Basic PRIA 210,00 190,00 - - -
-Prevalent PRIA 234,00 218,00 - - -
-Surveillance PRIA 367,00 337,00 - - -
-Surveillance Plus PRIA 409,00 373,00 373,00 361,00 301,00 301,00
FELASA Basic PRIA (3 months) 277,00 283,00 226,00 264,00 210,00
-FELASA Complete PRIA (Annual) 337,00 332,00 257,00 295,00 226,00
-Fecal PRIA 355,00 319,00 - - -
-Bacteria PRIA 301,00 283,00 - - -
-Custom PRIA Panel Upon request
Single Agent Test 80 € / agent*
* Except for Helicobacter genus (50 €) and Helicobacter screen (genus, bilis, hepaticus, typhlonius: 90 €).
Environmental Sampling
detect viruses, bacteria and parasites by screening ventilated caging system to augment sentinel monitoring programmemes. Panel Mouse Price - € Rat Price - €
Prevalent Environmental PRIA 234,00 226,00
Surveillance Plus Environmental PRIA 409,00 373,00
Complete Environmental PRIA 415,00 415,00
Custom Environmental PRIA Panel Upon request
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Health Monitoring (HM) Protocols
Whole animals can be submitted for a health monitoring (hm) protocol - samples will be collected in our necropsy laboratory and will be screened for the presence of infectious agents. also, services offered as part of a hm protocol are available individually - samples can be collected at your facility and directly submitted to our laboratory for testing. custom testing is available upon request.
Included TesTs
Protocol serology* PcR** Microbiology* Parasitology Pathology Pathology
HM Basic (None) (None) Upper
respiratory and gastrointestinal tracts included with all protocols. Endoparasite and ectoparasite exams included with all protocols. Gross necropsy with histology of lesions included with all protocols. Brain, kidney, and lung collection (included with all rabbit protocols.) HM Basic (Immunodeficient) (None) C. bovis and Pneumocystis (mouse only); Lawsonia (Hamster only)
HM Prevalent Prevalent (None)
HM Standard Tracking (None)
HM Assessment Assessment (None) HM Plus Assessment Plus (None) HM Plus without
Microbiology
Assessment
Plus (None) (None)
HM Quarterly FELASA FELASA quarterly Helicobacter Upper respiratory and gastrointestinal tracts Endoparasites and ectoparasites Gross necropsy and histology of lesion -HM Annually
FELASA FELASA annually Helicobacter
Upper respiratory and gastrointestinal tracts Endoparasites and ectoparasites Gross necropsy and histology of lesion -Sentinel Programme HM Standard
HM Standard or HM Plus profile on sentinels. • 2 nu/nu + 2 nu/+ SOPF sentinel mice. • or 2 SOPF sentinel rats.
Pathology, microbiology, parasitology performed on 2 nu/nu. Tracking (for HM standard) or
Assessment Plus (for HM Plus) serology on 2 nu/+.
PCR for Helicobacter in HM Plus.
Shipping crates containing hydration, food and bedding. Crate delivery. Animal pickup service available on request.
Sentinel Programme HM Plus
Custom Protocol Fully customisable, build-your-own protocols are available for any species; upon request. *Visit the online catalogue to view the list of agents included for each protocol and animal species.
**In addition to the included PCR tests, samples can be collected and screened for agent(s) of your choice (e.g. Helicobacter) for an added fee.
hm Protocol pricing includes all tests indicated in the table above.
Protocol Price - Mouse € Price - Rat € Hamster Price - € Guinea Pig Price - € Price - Rabbit € Price - Gerbil €
HM Basic 200,00 200,00 283,00 239,50 272,50 239,50 HM Basic (Immunodeficient) 350,00 350,00 - - - -HM Prevalent 255,00 255,00 - - - -HM Standard 281,50 287,50 - - - -HM Assessment 309,00 315,00 380,00 356,00 384,00 367,00 HM Plus 398,00 398,00 - - - -HM Quarterly FELASA 285,00 330,00 - - - -HM Annually FELASA 405,00 405,00 - - -
-Custom Protocol Upon request
Sentinel Programme HM Standard 785,00 785,00 - - -
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Serology Profiles
Blood samples collected at your facility can be submitted directly to our laboratory for testing. Protocol Mouse Price - € Rat Price - € Hamster Price - € Guinea Pig Price - € Rabbit Price - € Gerbil Price - € Miscellaneous Rodent Price - €* Parvovirus 67,00 67,00 - - - - -Prevalent 78,00 78,00 - - - - - Tracking 90,00 96,00 - - - - -
Assessment 119,00 129,00 107,00 129,00 132,00 138,00 Upon request
Assessment Plus 169,00 175,00 - - - - -
Custom Profile Upon request
Individual test
MFIA / IFA / ELISA 36,30 36,30 36,30 36,30 36,30 36,30 36,30
*For applicable species (e.g. Armenian hamster, cotton rat, ground squirrel), contact us (see page 6).
Microbiology Culture
swabs and other samples collected at your facility can be submitted directly to our laboratory for testing.
culture Price - Mouse € Price - Rat € Hamster Price - € Guinea Pig Price - € Price - Rabbit € Price - Gerbil €
Upper Respiratory 74,88 74,88 74,88 74,88 74,88 74,88
Gastrointestinal Tract 54,50 54,50 54,50 54,50 54,50 54,50
Custom Culture Upon request
Single Agent Culture Upon request
Rodent Parasitology
samples (e.g. feces, swabs or tapes) collected at your facility can be submitted directly to our laboratory for testing.
sample Type Test Price - €
Feces* and anal swab*
Faecal Concentration Centrifugation (FCC)* 50,00
Pinworm PCR (Aspiculuris and Syphacia) 50,00
Cryptosporidium PCR 50,00
Giardia PCR (G. lamblia, G. muris) 50,00
Spironucleus muris PCR 50,00
Fur swab* Furmite PCR (Myobia, Myocoptes, Radfordia). 50,00
Tape Tape Test for Ectoparasites 29,00
Tape Test for Endoparasites 29,00
Live animal Direct Exam for Ectoparasites 42,00
Direct Exam for Endoparasites 42,00
*Up to 8 samples for FCC and 10 for PCR can be pooled and tested as a single group with one result reported.
Pathology
as part of a hm protocol, gross lesions are collected for histopathology at no additional charge. Further, we offer full-service diagnostic histopathology, either with live-animal submission or by direct submission of fixed tissues, paraffin blocks or slides. We offer histologic evaluation of tissues from multiple species, complete tissue processing and slide preparation, and routine or specialised staining techniques, as well as contract slide preparation and evaluation. contact us for details and pricing, see page 6.
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Simian (Nonhuman Primate) Health Surveillance
services available for samples only; whole animals are not accepted.
service Item Price - €
Serology*
Herpes B virus only 45,75
Macaque Tracking 59,00
Macaque Assessment 94,75
Baboon Tracking 59,00
African Green Tracking 59,00
Infectious Disease PCR
Blood PCR Panel 356,50
Blood PCR – Custom Panel Upon request
Blood PCR – Single Agent 102,00
Fecal PCR Panel 244.50
Fecal PCR – Custom Panel Upon request
Fecal PCR – Individual Agent 80,00
Combination Panel
(Serology* and PCR) Combination screen – Individual Agent 120,25
Microbiology
Simian Cultures 110,65
SAL and SHIG 55,25
SAL 47,84
SHIG 47,84
CAMPY 58,50
YERS 58,50
Other Upon request
Parasitology Fecal Concentration Centrifugation 54,50
Histopathology Histopathology services Upon request
*Service available for Old World simian species only.
Ferret Health Surveillance*
charles River offers the following services to screen your ferret colonies:
• infectious disease PcR
• microbiology
• Parasitology
• histopathology
• clinical pathology
Zebrafish Health Surveillance*
charles River offers the following services to screen your zebrafish:
• infectious disease PcR
• microbiology
• Parasitology
• histopathology
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Serology Reagents for In-House
Animal Health Surveillance
commercial use of charles River reagents requires specific licensing. Please inquire for further details.
MFIA
®Reagents
MFIA® Bead Mixtureeach unit contains 0.3 ml and is sufficient for one plate of 96 tests.
Item Price - €
Mouse Parvovirus 1 497,60
Mouse Prevalent 2 808,00
Mouse Tracking 4 681,04
Mouse Assessment 6 864,00
Mouse Assessment Plus 8 799,44
Rat Prevalent 3 110,64
Rat Tracking 4 306,64
Rat Assessment Plus 7 450,56
Macaque Tracking 4 037,28
Macaque Assessment 6 458,40
Baboon Tracking 4 037,28
African Green Tracking 4 037,28
Rabbit Assessment 4 153,76
Guinea Pig Assessment 3 738,80
Hamster Assessment 2 579,20
MFIA® Control Sera Package
each unit contains 0.25 ml (high/low range positivity, nonimmune) and is sufficient for five plates.
Item Price - €
“Mouse A” for Parvovirus, Prevalent, Tracking 422,94
“Mouse B” for Assessment, Assessment Plus 1 371,30
“Rat A” for Prevalent, Tracking 416,85
“Rat B” for Assessment Plus 723,06
Macaque Tracking 936,60
Macaque Assessment 1 573,95
Baboon Tracking 936,60
African Green Tracking 936,60
Rabbit Assessment 725,92
Guinea Pig Assessment 378,56
Hamster Assessment 277,68
MFIA® Supplemental Reagents
each unit is sufficient for five plates.
Item Price
Conjugate for Mouse/Rat Samples 209,04
Conjugate for Simian Samples 534,24
Conjugate for Rabbit Samples 385,84
Conjugate for Guinea Pig Samples 196,56
Conjugate for Hamster Samples 579,28
Primary Diluent (Rodent and Rabbit) 187,20
Primary Diulent (Simian) 190,80
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ELISA Reagents
Item unit
each unit
Sufficient for: Price - €
96-well coated slide 1 plate 48 tests 221,52
Conjugate 0.2 ml 10,000 tests 51,94
Control sera - Positive (High or Low Score) 0.5 ml 10 plates 51,94
Control sera - Negative 0.1 ml 10 plates 21,84
IFA Reagents
Item unit Sufficient for:each unit Price - €
18-well coated slide 1 slide 18 tests 113,42
Conjugate 0.1 ml 180 tests 51,94
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Cell Line and Research Biologics Screening
charles River cleaR (cell line examination and Report) PcR Panels are performed non-gXP; this service is available for research purposes only.
Rodent CLEAR
detect the presence of rodent infectious agents.
Item Price - €
Mouse Essential CLEAR Panel 451,00
Rat Essential CLEAR Panel 394,00
Mouse/Rat Comprehensive CLEAR Panel 622,00
Custom CLEAR Panel* Upon request
Single Agent Test (Individual or Add-on) Upon request
*Rodent and human infectious agent tests can be combined into one custom panel.
Human CLEAR
detect the presence of human infectious agents.
Item Price - €
Human HEP/HIV CLEAR Panel 189,00
Human Essential CLEAR Panel 474,89
Human Comprehensive CLEAR Panel 530,00
Custom CLEAR Panel* Upon request
Single Agent Test (Individual or Add-on) Upon request
*Rodent and human infectious agent tests can be combined into one custom panel.
Contamination CLEAR
detect contamination of cell lines with cells of another species.
Item Price - €
Stand-Alone Service 76,96
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Environmental Monitoring
Microbial Bioburden / sterility Testing Price - €
Water Microbial/Bioburden 148,72
Sterility 63,44
Rodent Feed Microbial/Bioburden 148,72
Sterility 63,44
Rodent Bedding Microbial/Bioburden 148,72
Sterility 63,44
surface Testing Price - €
Environmental swab (culture) for rodent pathogens 148,72
Environmental swab (PCR) for rodent pathogens* Upon request
Surface swab (culture) for relative count 63,44
RODAC™ plate count 15,60
RODAC™ plate count with Gram stain 20,80
*PCR testing for the agent(s) of your choice. Price may vary with agent selection. Please contact us for details (see page 6). Pathogen Monitoring using PCR Rodent Infectious Agent (PRIA) Panels. For details, please see page 55.
Biomarker Testing Services:
Clinical Pathology and Immunology
samples can be collected at your facility and submitted directly to our laboratory for testing.
Clinical Pathology
Clinical Chemistry
visit the online catalogue to view the details including the list of analytes in each panel and all available single analyte tests. Item Price - € Lipid Profile 39,52 Liver Profile 44,72 Kidney Profile 46,80 Diabetes Profile 30,58 Iron Profile 45,58 Complete Profile 82,16
Custom Profile or Single Analyte Test (individual test or add-on) Pricing available online
Other Clinical Pathology Tests*
additional tests available (e.g. urinalysis); visit the online catalogue to view the comprehensive test list and pricing.
Immunology*
charles River offers fast, flexible testing of biomarker testing on sample types from small and large in vivo study models. testing can be performed on multiple sample matrices (e.g. blood, urine). Biomarker immunoassays are available on several platforms including enzyme-linked immunosorbent assay (elisa), luminex® and meso scale
discovery (msd®), covering a broad continuum of dynamic ranges and providing the ability to multiplex. custom
reports can be formatted to your specifications.
Item Price - €
Insulin 42,69
Adrenocorticotropic Hormone (ACTH) 75,00
Corticosterone 10,67
Custom Profile or Single Test Upon request
tests are available individually or combined into custom panels; pricing available upon request. On-Demand Immunoassay Development
charles River’s scientists will help to design and qualify study-specific biomarker assays and readouts for small and large research animal models. they can transfer and qualify assays or develop them for you. Pricing available upon request.
Geneticall
y
en
Gineered Models and
services
Genetically
enGineered Models
and services
For nearly 25 years, Charles River has delivered genetically engineered models and related services
to biopharmaceutical companies, world renowned academic institutions and a host of government
agencies globally. Today, GEMS (Genetically Engineered Models & Services) prides itself on being
a “one-stop shop” provider of integrated services, including customised breeding programmes,
quarantine space, genetic testing, rederivation, cryopreservation, IVF and proof of concept studies.
Contact us with your enquiry, please refer to page 6.
Genetically engineered Models and services
introducing icM™
Charles River is pleased to announce the launch of our Internet Colony Management (ICM™) system. ICM™ is a revolutionary, custom designed software solution for both project and vivarium manage-ment. Capturing thousands of data points using tablet computers and RFID, ICM™ prioritises tasks and drives day-to-day activities. The data collected in the vivarium is instantly made available to pro-ject managers and clients through an online portal, where clients also have the option of initiating animal-related tasks at any time.
ICM™ Features:
• Improved data integrity through simple,
source-captured data inputs
• Real-time data sharing among animal care and
project management staff and customers
• Two-way, data-rich customer portal to share
information and direct animal / colony requests
• Extensive filtering and sorting capabilities to
quickly navigate to the data you need
• Data export to Microsoft Excel® to allow for cus -tom data manipulation
• 360° data flow to ensure accuracy, confirm task
completion and manage colony goals In addition to its broad colony management fea-tures, ICM™ also serves as a repository of project
information, such as current and past goals, exten -sive details on each colony, health reports, ship-ment history and a complete record of any tasks you have requested. With ICM™, all of your animal and project information is just a mouse click away. To learn more about ICM™ or to view a video of the system in action, visit www.criver.com/icm
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y
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services
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Genetically Engineered Models and Services
Quarantine Services
dedicated, isolator-based space reserved for assessing the health profile of animals coming from outside institutions. charles river offers several different standard quarantine options and can develop custom protocols to meet animal facility requirements. Please contact us to discuss these custom options (see page 6).
Service Description
Estimated
Time Line Price - €
PRIA Quarantine Up to 10 mice 4 weeks 1 700,00
Standard Quarantine 18 cages isolator, including complete health report 11 weeks 4 520,00
Standard Breeding Services
off-site space for holding, breeding, and developing genetically engineered colonies. all colonies are assigned a dedicated project manager and clients are granted access to charles river’s innovative internet colony management (icm) system. icm provides a two-way communication portal with real-time data access, a comprehensive suite of colony management tools, and a complete historical record of all colonies housed at charles river. standard Breeding services are invoiced monthly depending on the real number of cages needed.
MouSE & RaT PRoDucTion coLony MainTEnancE includes*
BaSic caGE Price - €/week
GEnETic caGE Price - €/week**
MouSE RaT MouSE RaT
• Husbandry • Mating • Weaning
• Dedicated Project Manager
17,50 22,00 20,30 25,60
* Does not include weekly isolator maintenance (additional cost: 60 € /week/ isolator) and health monitoring controls.
** Sample collection for genetic testing.
Advanced Breeding Services
through the use of sophisticated techniques such as natural and laser-assisted in vitro fertilisation (ivF) and
intracytoplasmic sperm injection, animals can be produced more quickly and in greater quantities than with traditional methods. these techniques can also help overcome breeding difficulties.
Service Requirements Deliverables Estimated Time Line Price - €
Mouse Rapid Expansion
I (IVF)
5 males
(proven breeders):
8-15 weeks old
40 - 80 rederived animals delivered, with complete
health report* 15 weeks
171 € / animal produced
Mouse Rapid Expansion
II (IVF)
5 males
(proven breeders):
8-15 weeks old
81-120 rederived animals delivered, with complete
health report* 15 weeks
153 € / animal produced
Mouse Rapid Expansion
III (IVF)
5 males
(proven breeders):
8-15 weeks old
121+ rederived animals delivered, with complete
health report*
15 weeks 142 produced€ / animal
Mouse Rescue Package I
Recovery of a line that
is no longer producing
offspring using IVF All animals produced Project based 6 245,00
Mouse Rescue Package II (Mouse Rescue with Sperm Cryopreservation)
Recovery of a line that
is no longer producing
offspring using IVF and sperm cryopreservation
All animals produced
Sperm cryopreservation Project based 7 382,00
Long-Term Aging
Transgenic Models Project based
Animals housed to reach
specified age Project based Upon request
* Applicable for most strains
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Rederivation Services
rederivation can eliminate unwanted parasites, viruses, bacteria and other opportunistic agents from research colonies. similar techniques can also be used to recover previously frozen embryos or sperm.
Service Description Requirements Deliverables
Estimated
Time Line Species Price - € Embryo Rederivation I (Rapid Rederivation) For Heterozygous x Wildtype 5 males (proven breeders): 8-15 weeks old • Minimum 2 visibly pregnant embryo recipient females
• Conventional colony maintained for 4 weeks following delivery 7 weeks Mouse 3 315,00 Embryo Rederivation II (Standard Rederivation) For Heterozygous x Wildtype 5 males (proven breeders):
8-15 weeks old Minimum 10 offspring with
complete health report 4 months
Mouse 4 740,00 5 males (proven breeders): 8-15 weeks old Rat 8 235,00 Embryo Rederivation III For Homozygous x Homozygous 10 males (proven breeders)
8-15 weeks old and
10 females
4-9 weeks old • Expansion of Homozygous colony
• Minimum 10 offspring • Complete health report
6 months
Mouse 6 635,00
5 males (proven
breeders)
8-15 weeks old and
5 females 8-12 weeks old Rat 9 490,00 Embryo Reconstitution 3 recipient females 45-60 Embryos
• Any animals recovered (with no guarantee of quantity if embryos not cryopreserved by Charles River). Minimum 10 offspring if embryos frozen
and stored at Charles River
• Complete health monitoring 15 weeks Mouse 2 005,00 Rat 3 410,00 Sperm Rederivation I (Rapid Rederivation) For Heterozygous x Wildtype 4 males (proven breeders) 8-15 weeks old • Minimum 2 visibly pregnant embryo receipient females • Sperm cryopreservation 7 weeks Mouse 4 190,00 Sperm Rederivation II (Standard Rederivation) For Heterozygous x Wildtype 4 males (proven breeders) 8-15 weeks old • Minimum 10 offspring • Complete health report • Sperm cryopreservation 4 months Mouse 5 500,00 Sperm Reconstitution Live animal recovery from cryopreserved sperm
Sperm from stock frozen by Charles River or by a third party
• Any animals recovered (with no guarantee of quantity if sperm not cryopreserved by Charles River). Minimum 10 offspring if sperm frozen
and stored at Charles River
• Complete health
monitoring
15 weeks Mouse 3 860,00
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Cryopreservation Services
cryopreservation provides a permanent solution to archiving genetically engineered lines no longer being actively used as well as safeguarding valuable lines in the event of a problem with the health or genetics of the line, or a major disaster.
Service Description Requirements Deliverables Species Price - €
Embryo
Cryopreservation I For Heterozygous x Wildtype strain
5 males (proven
breeders) 8-15
weeks old • 300 embryos
Mouse 3 640,00 Rat 5 460,00 Embryo Cryopreservation II Homozygous strain undergoes preliminary expansion breeding 10 males (proven breeders) 8-15
weeks old and
10 females 4-9
weeks old • 150 embryos
• Homozygous expansion breeding
Mouse 3 640,00
5 males (proven
breeders) 8-15
weeks old and
5 females 8-12
weeks old
Rat 5 460,00
Sperm
Cryopreservation sperm from 2 malesCryopreservation of
4 males (proven
breeders) 8-15
weeks old
• Pre & post thaw QC • 15 straws preserved,
most strains Mouse 1 330,00
Cryopreservation Storage Secure Cryostorage in two independent facilities 415,00 € / year
I•CRYO™ Do-it-yourself sperm cryopreservation kit
• Instructions • Materials
• Cryo Media for 5 Lines • IVF QC Media for
5 Lines
• Cryostorage at Charles River (1 year)
Mouse 3 675,00
Model Creation Services
charles river provides core-lab support products and services to ease the process of model creation.
Product/Service Deliverables Price - €
BlastoKit® C57BL/6NCrl Small 400 C57BL6N/Crl embryos 3 365,00
BlastoKit® C57BL/6NCrl Large 1,600 C57BL/6NCrl embryos 11 915,00
BlastoKit® BALB/c-AnNCrl Small 400 BALB/cAnNCrl embryos 4 580,00
BlastoKit® BALB/c-AnNCrl Large 1,600 BALB/cAnNCrl embryos 16 325,00
BlastoKit® B6 Albino Small 400 C57BL/6N-Tyrc-Brd/BrdCrCrl embryos 4 015,00
BlastoKit® B6 Albino Large 1,600 C57BL/6N-Tyrc-Brd/BrdCrCrl embryos 14 315,00
BlastoKit® Custom Custom Quantity or Strain of Embryos Upon request
ES Cell Injection Package I ES cells injected into ~80 blastocysts, chimeric offspring returned with complete health report Upon request
ES Cell Injection Package II bred with wildtype animals to confirm germline transmission, ES cells injected into ~80 blastocysts, chimeric offspring positive F1 offspring returned with complete health report
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Genetic Testing Services
Full-service dna and rna based testing to identify common elements, gene of interest, expression levels, copy number, and background strain information. the charles river maX-BaX® speed congenics service can help develop
fully congenic strains in half the traditional time. New Genetic Testing Services Laboratory in Europe
the charles river genetic testing services are performed in their state-of-the-art facility in erkrath, germany. this facility has recently undergone a modernisation and capacity expansion allowing us to offer more flexibility to our customers. enhancements have also been made in terms of biosecurity with an improved workflow helping to speed turnaround time for providing testing results.
the charles river genetic testing services facilities are using their new online data management platform: ltm™ (laboratory testing management). ltm™ centralises genetic testing programmes in one virtual location with a user-friendly, webbased, secure interface. this robust software solution has many uses including easily duplicating orders for repeat testing, instant alerts and access to historical data.
Service Description Price - Sample€/assay/
Genotyping PCR for transgenic and KO/KI animals using gel based
conventional PCR
Zygosity testing Upon request
Genotyping including restriction
digest analysis E.g. point mutation detection in SCID mice Upon request
Q-PCR analysis Zygosity testing for transgenic lines Upon request
Relative copy number determination of transgenes Upon request
Assay development and optimization Conventional PCR 305,00
Q-PCR 715,00
SNP (Single Nucleotide
Polymorphism testing)* Analysis of genetic background 155,00
MAx-BAx® Speed Congenics* Fast development of congenic strains 265,00
* Services performed in United States.
