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Optimization of 3-D organotypic primary colonic cultures for organ-on-chip applications

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Figure

Figure 1 Isolation of crypts from a mouse colon. (A) Schematic of crypt isolation. The resected colon was incubated in chelating buffer,washed and then mechanically agitated
Figure 2 Effect of Matrigel concentration on(enteroendocrine marker: green).fluorescence.(blue) in panelseGFP fluorescence images of the same colonoid over 1 week in culture
Figure 3 Crypt-substrate interaction. (A)glass, oxidized polystyrene and epoxy photoresist, crypt-cells rapidly differentiate

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