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Infrared absorbance spectroscopy of aqueous proteins : comparison of transmission and ATR data collection and analysis for secondary structure fitting

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Figure

FIGURE 1A, Transmission absorbance spectra of water (55M),hemoglobin (approximately 100 mg/mL), concanavalin(approximately 20 mg/mL), and lysozyme (approximately100 mg/mL) in transmission mode with a cell with 4cell assembled to 7 μm spacer but–10 μm path
FIGURE 2A, Attenuated total reflectance spectra of water(55M), hemoglobin (10 mg/mL), concanavalin (10 mg/mL), andlysozyme (10 mg/mL) with a ZnSe ATR unit and 45° angle ofincidence
FIGURE 3Baseline‐subtracted spectra for, A, hemoglobin, B, concanavalin A, and, C, lysozyme all normalized to a maximum of 1 in theamide I band
FIGURE 4‐Wavelength dependence of the refractive index ofwater in the amides I and II regions overlaid with the correctionfactor used to correct aqueous protein attenuated total reflectance(ATR) spectra to resemble transmission spectra
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