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CARLSBERG RESEARCH LABORATORY ANNUAL REPORT 2020 SCIENTIFIC STAFF AT CARLSBERG RESEARCH LABORATORY

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Carlsberg Research Laboratory Carlsberg A/S

J. C. Jacobsens Gade 4 1799 Copenhagen V Denmark

CARLSBERG RESEARCH LABORATORY ANNUAL REPORT 2020

SCIENTIFIC STAFF AT CARLSBERG RESEARCH LABORATORY

Head of Carlsberg Research Laboratory, VP Group Research Birgitte Skadhauge

Director of Research Support & Project Management Anders Holme Jensen

Raw Materials platform Director

Kim Jørgensen

Professor (Associated) Geoff Fincher

Principal Scientists Finn Lok, Kenneth Fredlund Senior Scientists

Christoph Dockter, Pai Rosager Pedas Scientists and Postdocs

Jose Cuesta-Seijo, Hanne Thomsen, Magnus Rasmussen, Qiongxian Lu, Emiko Murozuka, Katarzyna Braune, Lucia Marri, Júlía Hafþórsdóttir, Morten Jørgensen, Iain Cooper, Jeppe Opstrup Husum, Sophia Tulloch

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Yeast & Fermentation platform Director

Jochen Förster

Senior Technical Brewer Birgitte Funch

Senior Scientists

Michael Katz, Claes Gjermansen, Rosa Garcia Sanchez, Klaus Lengeler Scientists and Postdocs

Ross Fennessy, Vratislav Stovicek, Kim Friis Olsson, Anna Chailyan PhD students

Isabella Jane Larsen

New Ingredients platform Director

Adam Fenton / Gustav Hambraeus

Distinguished Professor (Associated) Birger Lindberg Møller

Principal Scientists Pia Vaag

Senior Scientists

Ilka Braumann, Renil Manat, Jesper Harholt Scientists and Postdocs

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Brewing Science & Technology platform Director Zoran Gojkovic Head Brewer Erik Lund Principal Scientists Arvid Garde, Søren Knudsen Senior Scientists

Jeppe Frank Andersen Scientists and Postdocs

Michela Della Negra, Iuliana Stoica, Stefano Soprani, David Martinez, Ali Farsi, Yanyan Su, Rolf Ringborg, Ole Mikkelsen, Helena Friis, Kathrine Ørnslund PhD students

Anders Bagger Sørensen

Commercial Unit Director

Gustav Hambraeus Senior Scientists Toni Wendt

Scientists and Postdocs

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***

PEER-REVIEWED PUBLICATIONS

Betts, N.S., Collins, H.M., Shirley, N.J., Cuesta-Seijo, J.A., Schwerdt, J.G., Phillips, R.J., Finnie, C., Fincher, G.B., Dockter, C., Skadhauge, B., Bulone, V. (2020): Identification and spatio-temporal expression analysis of barley genes that encode putative modular xylanolytic enzymes. Plant Science, 110792.

Betts, N.S., Dockter, C., Berkowitz, O., Collins, H.M., Hooi, M., Lu, Q.X., Burton, R.A., Bulone, V., Skadhauge, B., et al. (2020): Transcriptional and biochemical analyses of gibberellin expression and content in germinated barley grain. Journal of Experimental Botany, 71, 6, 1870-1884.

Colomer, M.S., Chailyan, A., Fennessy, R.T., Olsson, K.F., Johnsen, L., Solodovnikova, N., Förster, J. (2020): Assessing Population Diversity of

Brettanomyces Yeast Species and Identification of Strains for Brewing Applications.

Frontiers in Microbiology, 11, 637.

Colomer, M.S., Funch, B., Solodovnikova, N., Hobley, T.J., Förster, J. (2020): Biotransformation of hop derived compounds by Brettanomyces yeast strains.

Journal of The Institute of Brewing, 126, 3, 280-288.

Iaccarino, N., Khakimov, B., Mikkelsen, M.S., Nielsen, T.S., Jensen, M.G., Randazzo, A., Engelsen, S.B. (2020): Structurally different mixed linkage beta-glucan supplements differentially increase secondary bile acid excretion in

hypercholesterolaemic rat faeces. Food & Function, 11, 1, 514-523.

Knudsen, C., Bavishi, K., Viborg, K.M., Drew, D.P., Simonsen, H.T., Motawia, M.S., Møller, B.L., Laursen, T. (2020): Stabilization of dhurrin biosynthetic

enzymes from Sorghum bicolor using a natural deep eutectic solvent.

Phytochemistry, 170, 112214.

Lengeler, Klaus B., Stovicek, V., Fennessy, R.T., Katz, M., Förster, J. (2020): Never Change a Brewing Yeast? Why Not, There Are Plenty to Choose From.

Frontiers in Genetics, 11, 582789.

Long, L.Z., Pedas, P.R., Kristensen, R.K., Schulze, W.X., Husted, S., Zhang, G.P., Schjoerring, J.K., Yuan, L.X. (2020): High light intensity aggravates latent manganese deficiency in maize. Journal of Experimental Botany, 17, 19, 6116-6127.

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Matyszczak, I., Tominska, M., Zakhrabekova, S., Dockter, C., Hansson, M. (2020): Analysis of early-flowering genes at barley chromosome 2H expands the repertoire of mutant alleles at the Mat-c locus. Plant Cell Reports, 39, 1, 47-61. Mnich, E., Bjarnholt, N., Eudes, A., Harholt, J., Holland, C., Jørgensen, B., Larsen, F.H., Liu, M., Manat, R., et al. (2020): Phenolic cross-links: building and de-constructing the plant cell wall. Natural Product Reports, 37, 7, 919-961.

Rustgi, S., Skadhauge, B. (2020): Diter von Wettstein, Professor of Genetics and Master of Translating Science into Applications, Methods in Molecular Biology, 2124, 3-18.

Skadhauge, B. (2020): Advancing Brewing Science. Proceedings 36, 1, 123. Presented at The Third International Tropical Agriculture Conference, Brisbane, Australia.

Xu, W.L., Jin, B., Zhou, S.F., Su, Y.Y., Zhang, Y.F. (2020): Triclosan Removal in Microbial Fuel Cell: The Contribution of Adsorption and Bioelectricity Generation.

Energies, 13, 3, 761.

Zhang, C.S., Kang, X.X., Wang, F.H., Tian, Y.F. Liu, T., Su, Y.Y., Qian, T.T., Zhang, Y.F. (2020): Valorization of food waste for cost-effective reducing sugar recovery in a two-stage enzymatic hydrolysis platform. Energy, 208, 118379. Zhao, N.N., Su, Y.Y., Angelidaki, I., Zhang, Y.F. (2020): Electrochemical capacitive performance of intact anaerobic granular sludge-based 3D bioanode.

Journal of Power Sources, 470, 228399.

Zhou, H.H., Xing, D.F., Xu, M.Y., Su, Y.Y., Zhang, Y.F. (2020): Biogas upgrading and energy storage via electromethanogenesis using intact anaerobic granular sludge as biocathode. Applied Energy, 269, 115101.

*** MASTER THESES

Cobo, M.F.: Brewery Wastewater Treatment and Nutrients Recovery by Microalgae, Technical University of Denmark (DTU), February 2020.

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Mikkelsen, O.: Non-Saccharomyces yeast for beer brewing with special focus on usability of Yarrowia in the production of sour beer, University of Copenhagen (UCPH), July 2020.

Thorball, N.A.: Co-cultivation of Hydrogen Oxidizing Bacteria and Microalgae for Brewery Wastewater Treatment, Technical University of Denmark (DTU), January 2020.

***

PUBLISHED PATENT APPLICATIONS AND GRANTED PATENTS

Donaldson, I., Vaag, P., Gojkovic, Z.: Method for producing beverages by acid removal, Patent no.: 3434113 (European Patent).

Møller, B., Mikkelsen, M., Jensen, M., Gojkovic, Z.: Beverages containing barley Beta-glucan, Patent no.: 3451852 (European Patent).

Møller, B., Mikkelsen, M., Jensen, M., Gojkovic, Z.: Beverages containing barley Beta-glucan, Patent no.: 748105 (New Zealand).

Riis, P., Knudsen, S., Skadhauge, B., Bech, L.M., Olsen, O.: A method for preparing a barley, a barley plant, a malt composition, and a barley based beverage prepared from a barley plant, Patent no.: 23028 (Vietnam).

Riis, P., Knudsen, S., Skadhauge, B., Bech, L.M., Olsen, O.: Energy saving brewing method, Patent no.: BR 11 2012 030806-8 B1 (Brazil).

Riis, P., Knudsen, S., Skadhauge, B., Bech, L.M., Olsen, O.: Energy saving brewing method, Patent no.: 344135 (India).

Singh, S., Jakob, M.: Fermentation tank with side ports and method of operation, Patent no.: 036267 (Eurasian Patent).

Singh, S., Jakob, M.: Fermentation tank with side ports and method of operation, Patent no.: 3452571 (European Patent).

Solodovnikova, N., Andersen, J.F., Sanchez, R.G., Gojkovic, Z.: Yeast for preparing alcoholic beverages, Patent no.: 2015371749 (Australia).

Solodovnikova, N., Andersen, J.F., Sanchez, R.G., Gojkovic, Z.: Yeast for preparing alcoholic beverages, Patent no.: 3237601 (European Patent).

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Wendt, T., Krucewicz, K., Marri, L., Skadhauge, B., Lok, F., Knudsen, S., Olsen, O.: Refined Cereal-based Beverages, Patent no.: 3478813 (European Patent).

Wendt, T., Krucewicz, K., Marri, L., Skadhauge, B., Lok, F., Knudsen, S., Olsen, O.: Refined Cereal-based Beverages, Patent no.: 19131 (OAPI).

Wendt, T., Krucewicz, K., Marri, L., Skadhauge, B., Lok, F., Knudsen, S., Olsen, O.: Refined Cereal-based Beverages, Patent no.: 11201810753S (Singapore).

Wendt, T., Krucewicz, K., Marri, L., Skadhauge, B., Lok, F., Knudsen, S., Olsen, O.: Refined Cereal-based Beverages, Patent no.: 2018/08217, (South Africa).

Wendt, T., Thomsen, H.C., Striebeck, A., Rasmussen, M.W., Carciofi, M., Olsen, O., Knudsen, S., Skadhauge, B.: Method of identifying a sub-pool comprising a mutant organism, Patent no.: 2019268102 (Australia).

Wendt, T., Thomsen, H.C., Striebeck, A., Rasmussen, M.W., Carciofi, M., Olsen, O., Knudsen, S., Skadhauge, B.: Method of identifying a mutant plant, Patent no.: 2019268103 (Australia).

References

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