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An alternative novel tool for DNA editing without target sequence limitation: the structure guided nuclease

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Figure

Fig. 1 Experimental strategies performed in vitro and in vivo. a Experimental strategies performed in vitro
Fig. 2 SGN cleaved ssDNA in vitro. Denatured-PAGE showed the DNA products produced by SGN cleavage
Fig. 3 The effect of different unpaired 3′ nucleotides on the DNA cleavage created by SGN
Fig. 4 The effect of the length of the gDNA on the DNA cleavage by SGN. Schematic (top) shows the gDNAs next to the ssDNA targets
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