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Global regulation of mRNA translation and stability in the early Drosophilaembryo by the Smaug RNA binding protein

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Figure

Figure 1 Identification of Smaug-bound mRNAs.across three biological replicates and one technical replicate, of themicroarray signal intensities of each expressed transcript in the Smaugand control RIPs divided by the signal intensities of each transcript
Figure 2 Validation of Smaug-bound mRNAs. The fold enrichment of mRNAs in Smaug RIPs versus control RIPs was determined via RT-qPCRand normalized to the levels of RpL32 mRNA in the immunoprecipitated material
Figure 4 Identification of the targets of Smaug-mediated translational repression. (A) The averages, across three biological replicates, ofthe TI in smaug-mutant and wild-type embryos were plotted against one another
Figure 5 Smaug represses the translation of thousands of mRNAs in the early embryo. (A) The 500 bottom Smaug binders are the 500genes whose mRNAs show the lowest fold enrichment in Smaug RIPs versus control RIPs and they were plotted as in Figure 4A
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