Product Focus November

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T^ARGET-VALIDATED AND CHARACTERIZED IVDA^NTIBODIES

FOR PATHOLOGY AND IMMUNOTHERAPY

Product Focus November

Zeta is very excited and proud to share IVD antibodies researched and developed for Anatomic Pathology market for Immunohistochemistry. Zeta is incorporating highly sensitive technology to develop many of these Monospecific primary antibodies that are Target-Validated and Characterized for IHC on FFPE tissue sections.

Zeta provides over 300 IVD antibodies for cancer targeted therapy and immunotherapy due to gene mutations, chromosomal translocations or gene amplifications.

CA 19-9 Mouse monoclonal Antibody IVD

Anti-mouse: Clone 121SLE, Cat # Z2072

CA19-9, a carbohydrate epitope expressed on a high MW (>400kDa) mucin glycoprotein, is a sialyl Lewis a structure which is synthesized from type 1 blood group precursor chains and is present in individuals expressing the Lewis a and/or Lewis b blood group antigens. In normal tissues, sialyl Lewis a antigen is present in ductal epithelium of the breast, kidney, salivary gland, and sweat glands. Its expression is greatly enhanced in serum as well as in the majority of tumor cells in gastrointestinal (GI) carcinomas, including adenocarcinomas of the stomach, intestine, and pancreas.

Preoperative elevated CA19-9 levels in patients with stage I pancreatic carcinoma decrease to normal values following surgery.

When used serially, CA19-9 can predict

recurrence of disease prior to radiographic or clinical findings.

Colon carcinoma stained with anti-CA19-9 antibody

*Reference:

1. Wu E, et al. Clin AdvHematol Oncol. 2013; 11:53–5.

2. Remmers N, et al. Clin Cancer Res.2013; 19:1981‐93.

3. Terada T. Int J ClinExp Pathol. 2013; 6:630‐8.

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Chromogranin A Mouse Monoclonal Antibody IVD

Anti-mouse: Clone ZM12, Cat # Z2347

Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.

Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti- chromogranin staining, and absence of anti- keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.

Pancreatic neuroendocrine tumor stained with anti- Chromogranin A antibody

*Reference:

1. Fischer‐Colbrie R, et al. Neuroscience. 1985; 16:547‐55.

2. Hearn SA. J Histochem Cytochem. 1987; 35:795‐801.

3. Wilson BS, et al. Am J Pathol. 1984; 115:458‐68.

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DPC4 (SMAD4) Mouse monoclonal Antibody IVD

Anti-mouse: Clone: B-8, Cat # Z2240

Signaling from the ligand-activated membrane receptor serine/threonine kinases to nuclear targets is mediated by a set of evolutionarily conserved proteins known as DPC4. Upon ligand binding, the receptors of the TGF-β family phosphorylate SMAD proteins (SMAD1 and SMAD2). These proteins then move into the nucleus, where they activate transcription. To carry out this function, the receptor activated SMAD1 and 2 require association with the product of deleted in pancreatic carcinoma, locus 4 (DPC4), also known as SMAD4.

SMAD4/DPC4 is also implicated as a tumor suppressor, since it is inactivated in more than half of pancreatic carcinomas and to a lesser extent in a variety of other cancers. The lack of SMAD4 expression is present in approximately 80% of cases of pancreatic adenocarcinoma, but rarely in endometrial (0%), colorectal (0%), ovarian (3%), lung (0%), breast (2%) adenocarcinomas, and malignant melanoma (4%). SMAD4 is an important marker for confirming a diagnosis of pancreatic adenocarcinoma.

*Reference:

1. Iacobuzio‐Donahue CA, et al. Am J Surg Pathol. 2000; 24:1544‐8.

2. Hornick JL, et al. Am J Surg Pathol. 2005; 29:381‐9.

Bile duct adenoma stained with anti-DPC4 antibody

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Insulin Mouse Monoclonal Antibody IVD

Anti-mouse: Clone ZM83a & ZM83b, Cat # Z2525

Antibodies recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets.

Antibodies to insulin are important as beta- cell and insulinoma marker.

A pancreatic islet stained with anti-Insulin antibody

*Reference:

1. Akagi T, et al. Cancer. 1981; 47:417-24.

2. Azzoni C, et al. Virchows Arch. 1998; 433:495-504.

3. Lubensky IA, et al. Am J Pathol. 1998; 153:223-31.

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Synaptophysin Mouse Monoclonal Antibody IVD

Anti-mouse: Clone: ZM208, Cat # Z2544

This antibody recognizes a protein of 38kDa, identified as synaptophysin. It labels normal neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, gastrointestinal mucosa, Paneth’s cells the gastrointestinal tract and of gastric parietal cells. Neurons in the brain, spinal cord, and retina are labeled.

In combination with anti-chromogranin and anti-NSE, Ab to synaptophysin is very useful the identification of normal neuroendocrine cells neuroendocrine neoplasms.

Pancreatic neuroendocrine tumor stained with anti- Synaptophysin antibody

*Reference:

1. Wiedenmann B, et al. Cell. 1985; 41:1017‐28.

2. Lyda MH, et al. Hum Pathol. 2000; 31:980‐7.

3. Skacel M, et al. Appl Immunohistochem Mol Morphol. 2000; 8:302‐9.

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All of our antibodies work on formalin-fixed paraffin embedded (FFPE) tissue sections. As an ISO 13485:2016 certified biomedical company, all our antibody clones are scientifically selected to fit the need of clinical immunohistochemical laboratories. Our primary antibodies are manufactured by FDA certified GMP facilities in the USA and purified by affinity chromatography with >99% purity.