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Essential kinase independent role of a Fer like non receptor tyrosine kinase in Caenorhabditis elegans morphogenesis

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Fig. 1. Zygotic loss of FRK-1 blocks epidermal enclosure and elongationat approximately 8.5 hours post-fertilization
Fig. 3. FRK-1 is required for late epidermal differentiation. (A)Percent of embryos with a wild-type (empty bars) and frk-1(RNAi)(filled bars) genotype expressing the indicated marker
Fig. 4. Quantification of rescue experiments with homozygous mDf7embryos. Percent embryonic lethality (empty bars) and enclosuredefective (filled bars) in progeny from mDf7 heterozygotes is shown.All mDf7 homozygous embryos (25% of the progeny for this fully
Fig. 5. FRK-1 kinase activity is not required for enclosure andelongation. (A) In vitro assays demonstrate the autophosphorylationactivity of FRK-1, which is eliminated in the FRK-1(D308R) mutant.The enclosure and elongation defects of mDf7 homozygous embryos(B) are rescued by expression of the kinase-dead form of FRK-1 (C).The rescued embryos express the seam-cell-specific marker, NE2-1B4 (C), as is seen with rescue by wild-type FRK-1.
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