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IPF1, a homeodomain protein with a dual function in pancreas development

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Figure

Fig. 3 Expressionof the IPF1recombinant expression vectors were co-transfected withand 5xP1malizedkind permission fromtransactivatesinternalcal/ycel/s
Fig. 4. IPF1affinity purified anti-IPF1 antibodiesis restrictedtotheB.-cells of adult mouse pancreas.Cryostat sections(8 urn) from adult mousepancreaswere stainedwithand anti-islet hormoneantibodies.(A) Staining (dark brown) with anti.fPF1 antisera showing
Fig. 5. IPF1 is specificallyexpressedferentiation.somite20-somitein both thedorsaland ventralgut wallof earlymouseembryosprior to morphogenesisand cytodif-Whole~mountimmunohistochemistryon wild-typemouseembryos(A,B,C,E,F) using anti-IPFlantisera.(A) 10-somiteembryo,(B) 15~embryo,IC) 2CJ.-somite embryo,(E) 27-somiteembryo,IF) 35-somiteembryo.(D) Immunohistochemistryon a 10 Jl cryostat sectionfrom aembryousing anti-IPF1 antisera.Magnificationx200.
Fig. 6. Ipf1 deficientmice lack a pancreas.IA) A pair of newborn littermares, wild-rypef+/+J and Ipl7 (-1-)mutant.(B) PhotOgraph ofthe stomachand duodenalregion of a new-born wild-type mouse85itappearsafterremoval from the abdomen.The arrows indi-cate the
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