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The role of stem cell factor and of alternative c kit gene products in the establishment, maintenance and function of germ cells

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Academic year: 2020

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Figure

Fig. 1. Schematic representation of two distinct c-kit gene products
Fig. 2. Western blot analysis of equal amounts of protein extracts frompurified male germ cell populations obtained from mouse post-natal testisat different stages of differentiation, probed with a polyclonal antibodydirected against the 13 carboxy-terminal amino acids encoded by themouse c-kit open reading frame.
Fig. 3. Western blot analysis of equal amounts of protein from soluble(cytosol), detergent solubilized (Triton X-100) and detergent-insoluble(SDS) extracts from control COS cells (mock) or from COS cells producingrecombinant tr-kit after transfection with a eukaryotic expression plasmid(tr-kit).
Fig. 4. Schematic representation of possiblemechanisms through which spermatozoa canγegg-sperm binding, a tyrosine-kinase receptor orsible for the following events of egg activation:tively, after sperm-egg fusion, a sperm factorond messengers IPcellular st

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