BioNordika Oy (Finland) • tel 0207 410 270 • [email protected] • www.bionordika.fi
January - March 2021
Issue 1
LOADED WITH COOL STUFF:
• Cryo storage consumables!
• Cryo storage instruments and LN2 tanks!
• New Qool supplier – QKine!
• Luna qPCR offer!
• Great offer for Flow antibodies & dyes!
2
Do you and your samples keep cool in tough situations?
Ask more from
Mirja!
Mirja Heinonen
Tel. 020 7410 279
Refrigerated Centrifuge 5425 R is the new laboratory standard for temperature sensitive samples up to 5 mL.
Centrifuges, tubes, pipettes, tips? Let’s talk!
Festivo-Porkka Scientific - A new generation of
healthcare and research refrigeration equipment
• The new generation of refrigeration equipment for healthcare and research.
• Wide range of refrigerators and freezers, as well as ice cubes and ice makers for the pharmaceutical industry, hospitals and research institutes.
• The healthcare and research product range has been designed and manufactured in its entirety for professional use and to meet the most stringent requirements of customers and the EU F-Gas Regulation.
• The versatile range of healthcare and research refrigeration equipment is high-quality and easy to use, and thanks to its low energy consumption, it is also environmentally friendly.
• Quicker pre-cooling from
~ 21° C to 4° C in only eight
minutes reducing waiting times during the workflow Aerosol-tight Eppendorf
QuickLock®
• Extended rotor portfolio of 6 rotors including 5 mL tube rotor and swing-bucket rotor for PCR strips
• OptiBowl® design for quiet operation, even without
rotor lid
Order any Porkka
instrument from us and
get full box of ice cream
3
Let's talk
...about safe freezing of cells
TheraPEAK ProFreeze freezing medium
• Universal freezing medium, especially for cells grown serum-free
• No animal-origin components
• Maintains high cell viability
• Addition of DMSO required, final concentration 7,5%
Valid until 31.03.2021 with promo code BN-WINTER21
Contact Hanna for
more information
Hanna Laiho
Tel. 020 7410 280
Part no. Product Pack
size Price (0% VAT) B75-SQ Storage box, cardboard, 3 in/76 mm 1 4,65 EUR
B95-SQ Storage box, cardboard, 4 in/102 mm 1 4,65 EUR
D64 Divider for cardboard storage box, 8x8 1 3,94 EUR
D81 Divider for cardboard storage box, 9x9 1 3,94 EUR
802202 Cryo rack for 81 CRYO.S, PP, natural, lid natural 20 94 EUR
BOXES AND DIVIDERS
Part no. Product Pack size Price (0% VAT)
BEBP12-769E TheraPEAKTM ProFreeze Freezing Medium, Chemically Defined 100 ml (2X) 180 EUR
12-132A Cryoprotective Freezing Medium 100 ml (2X) 85 EUR
12611S DMSO (Dimethyl Sulfoxide), Sterile 5 x 10 ml 169 EUR
NEEDED FOR SAFE FREEZING
High quality cryo vials for every need
• 1, 2, 4 or 5 ml
• Internal or external thread
• Round or conical bottom
• Starfoot base available
• Ask for triple packed for cleanroom environment
• Available with datamatrix and linear barcode
Buy a package of Greiner cryovials and
get an alcohol and water-resistant cryomarker
free of charge!
Cryo storage boxes in
4 Valid until 31.03.2021 with promo code BN-WINTER21
...about cryo storage
Safe shipping in liquid or vapor phase below -150°C
Contact Hanna!
Hanna Laiho
Tel. 020 7410 280
• Economical one-way dry shippers charge in 2 hours: safe shipping in any orientation, holds cold 5/7 days
• Conform to MDD 93/42/EEC directive
• Safe for shipping by air by IATA
More reproducible thawing
process with SmartBlock cryo
• Controlled and reliable cell cryo vial thawing
• Prevents contaminations
• Cell-specific protocol possible
• Documentable and traceable process
• Clean-room friendly
• Exchangeable thermoblock is
compatible with both ThermoMixer C
and ThermoStat C
NEW!
Long term cryo storage at -190°C
• Aluminum tanks for storing vials or straws
• Conform to MDD 93/42/EEC directive
• 5 year vacuum warranty
• SC for small amount of samples, working duration up to 116 days
• XC for larger amount of samples, working duration up to 193 days
• CryoSystem for storage in boxes, working duration up to 104 days
Super-insulated dewars for
storing and dispensing
of li quid nitrogen
• Low evaporation rate
• Fit with a pouring sprout or pressurized dispensing pump for safe transfer of LN2
General
Roller Base
-50% with
any MVE
tank
5
CELLEDYRKNING
Valid until 31.03.2021 with promo code BN-WINTER21
Let's talk
...about tools for cell culture
ToxiLight™ non-destructive cytotoxicity bioassay kit
The ToxiLight BioAssay Kit is a bioluminescent, non-destructive cytolysis assay kit designed to measure the release of the enzyme, adenylate kinase (AK), from damaged cells. As the level of cytolysis increases, the amount of AK in the supernatant also increases, which results in emission of higher light intensity by the ToxiLight reagent. Because the ToxiLight BioAssay Kit exploits the fact that AK is released from cells when they die, there is no need for cell lysis (unlike many other cytotoxicity assays). Repeated samples of supernatant can therefore be taken over time without disrupting the cells themselves. This allows for kinetic analysis of cell death.
Cat. no. Product
LT07-117 ToxiLight BioAssay Kit, 1000 tests LT07-217 ToxiLight BioAssay Kit, 500 tests
LT07-517 ToxiLight 100% Lysis Control Set, 200 tests LT17-217 ToxiLight BioAssay Kit with Plates, 500 tests
-20%
on all ToxiLight
products
Lonza's MycoAlert kit detects viable Mycoplasma in less than 20 minutes. A small sample of the cell culture media is used with a two-step protocol: Add a reagent, measure, add substrate, and measure. The ratio between the two measurements indicates if the sample is contaminated or clean.
Mycoplasma detection
-10%
on all MycoAlert
products
The products from Qkine have high biochemical quality for stem cells, organoid culture and regenerative medicine applications. All products have high purity and bioactivity, are manufactured using animal-free processes, and are optimized for scalability.
Some of the products available today:
• Core Stem Cell Growth Factors: Activin A, hFGF2/bFGF, BMP2, EGF, TGFβ1 PLUS, HGF NK1,
mouse LIF
• Organoid Growth Factors: R-spondin-1, R-spondin-3, Gremlin-1, FGF4, FGF10
• TGFβ family biomarkers: GDF15
Products coming soon:
• Core & optimised stem cell growth factors: BMP4, human LIF, mouse & human Noggin, OSM, FGF7, IGF1 and stabilised FGF2
• Neuronal growth factors: BDNF, GDNF, GM-CSF, NGF
• Haematopoeitic growth factors: IL-3, IL-6 and IL-34
NEW supplier: Qkine
- specializes in growth factors and cytokines
Want to test the MycoAlert PLUS assay?
Request a trial kit at a 50% discount!
-20%
Introductory
offer
on all products
6
...about supporting COVID-19 research
Valid until 31.03.2021 with promo code BN-WINTER21
NEB Monarch HMW DNA Extraction Kits
Do you feel like the best thing to do when it’s cold and dark would be to retreat for a long hibernation? Still, we humans keep up the pace and stay active through the winter. While staying awake, take the new NEB Monarch HWM DNA Extraction Kit as your companion. The High Molecular Weight kit love DNA into the Mb size range and we are sure it can give some of its energy to you.
Take the chance and enjoy the long winter months together with HMW kits!
NEB has launched two Monarch HMW DNA Extraction Kits: one for extraction of high molecular weight genomic DNA from cells and blood (NEB #T3050), and another for tissue and bacteria (NEB #T3060). These kits employ a novel glass bead-based approach, allowing users to extract DNA from 50kb up to several megabase (Mb) quickly and easily, with best-in-class yields and purity
• Fast workflows (cells: 30 minutes, blood: 60 minutes, tissue & bacteria: 90 minutes
• Tune DNA size based on agitation speed during lysis
• Excellent performance in long read sequencing
NEB Restriction Enzymes
If there would be a chance to cut the winter short would you do it? Usually, it would be done easily by taking a flight somewhere warm. In this strange COVID-19 time it’s more difficult.
While NEB restriction enzymes does not offer a solution to this challenge, they stay active despite the winter and cut at the right spot. Choose the right restriction enzymes for your research, sink into your work and soon you will realize winter and COVID-19 has been left behind.
Ask Pia!
Pia Ojala [email protected] Tel. 020 7410 274NEW!
-10%
on all restriction
enzymes from
NEB
• NEB has over 40 years of experience with restriction enzymes
• NEB enzymes have high quality and reliability
• Choose from >285 restriction enzymes
• Simplify reaction setup and double digestion with CutSmart Buffer, a single buffer for over 210 restriction enzymes
• >195 restriction enzymes are Time-Saver qualified
• Choose a High-Fidelity (HF®) restriction enzyme, which has been engineered for reduced star activity, rapid digestion (5-15 minutes) and 100% activity in CutSmart Buffer
7
CELLEDYRKNING
Let's talk
...about going low
Valid until 30.6.2020 with promo code BN-SPRING20
Valid until 31.03.2021 with promo code BN-WINTER21
NEBNext Single Cell/Low Input RNA Library Prep
Did you know that Emperor penguins can dive for almost 30 minutes on a single breath and as deep as 457 meters? Their heart-rate can be as low as three beats per minute. Decreased heart heart-rates during dives correspond to conservation of oxygen, enabling the animals to dive for a longer time (1).
With NEB’s NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina you can generate the highest yields of high-quality sequencing libraries from single cells, or as little as 2 pg - 200 ng RNA. Impressively low for both penguins and NEBNext!
• Experience unmatched detection of low abundance transcripts
• Rely on consistent transcript detection for a wide range of input amounts and sample types
• Observe uniform transcript coverage, regardless of input amount or sample type
• Use with a variety of RNA inputs, including cultured or primary cells, or total RNA
• Save time with a fast, streamlined workflow, minimal handling steps and hands-on time
1. Meir, Jessica 2009: How Penguins & Seals Survive Deep Dives. National Science Foundation. 4.1.2021.
HTG EdgeSeq
HTG EdgeSeq system simplifies gene expression profiling and enables use of ultra low sample input
amounts.
HTG’s extraction-free solution enable fast, reliable, reproducible, and quantitative analysis of thou-sands of targeted RNAs from a single assay in as little as 36 hours.
HTG EdgeSeq chemistry allows for multiplexed, quantitative molecular profiling using a 5 µm FFPE tissue section or 15 µl of plasma/serum.
HTG EdgeSeq NGS workflow
How low
Valid until 31.03.2021 with promo code BN-WINTER21 8
...about bringing light at dark
Lighting the way!
The winter is long, even if there is snow. Let Luna light up your lab! Luna products from NEB are optimized for qPCR or RT-qPCR and are available for either inter-calating dye or probe-based detection methods. All Luna products provide robust performance on diverse sample sources
and target types.
Each Luna qPCR master mix has been
formulated with a unique passive reference dye that is compatible across a wide variety of instrument platforms, including those that require a ROX reference signal. This means that no additional
components are required to ensure
machine compatibility.
Let me help you!
Pia Ojala [email protected] Tel. 020 7410 274
-10%
on LUNA
products from
NEB
Find the right Luna product for your application:
LUNA products for
qPCR or RT-qPCR
NEW!
Now available a
real-time RT-PCR assay for
the qualitative detection
of SARS-CoV-2 nucleic
acid:
Luna SARS-CoV-2
RT-qPCR Multiplex
Assay Kit (E3019)
Multiplex detection of 2019-nCoV_N1 and 2019-nCoV_N2
targets and human RNase P gene enables high through -put workflows
If there is no light to
be found, then cut the
darkness with
NEBNext RNA
Depletion Core
Reagent Set!
It is a customized
approach to delete
unwanted RNA from
any organism using
probe sequnces design
with the online tool.
Valid until 31.03.2021 with promo code BN-WINTER21 9
CELLEDYRKNING
Let's talk
...about crystallography
NexTal crystallization plates
The EasyXtal crystallization plates are used for manual setup of hanging-drop protein crystallization trials in microliter volumes.
• Excellent optical properties and format for automatic visualization systems
• Highly suitable for hit optimization and ligand screening
• Unique, easy-to-open, greaseless, screw-in crystallization supports
• Highly suitable for structure-based drug design optimization and screening
Maximize your screening potential with the Morpheus
screen family from Molecular Dimensions
Talk to Juho!
Juho Niva [email protected]
Tel. 020 7410 276
The Morpheus family of rational screens is exclusively available from BioNordika. Designed from scratch and optimized against a range of protein samples to avoid bias by Molecular Dimensions. The Morpheus screens are available in multiple formulations and they have been widely used for crystallizing of both soluble and membrane proteins.
The Morpheus screen family includes:
• Morpheus® screen
• Morpheus® II screen
• Morpheus® III screen
• Morpheus® additive Screen
• MHippocrates™ additive screen
• LMB Crystallisation Screen
• Angstrom Additive Screen
-10%
on all Morpheus
screen family
products
C-flat Carbon Grids for Cryo-EM
C-Flat is an ultra-flat carbon support grid, optimized for Cryo-Electron Microscopy (CRYO-EM). Create 3D constructions with ease: just choose the best grid for your research and with C-Flat ultra-flat grids you’re ready to collect data immediately
- no pre-washing!
-10%
C-flat carbon grids
&
EasyXtal plates
Valid until 31.03.2021 with promo code BN-WINTER21 10
...about Flow Cytometry
New record in spectral flow cytometry
with CF® dyes!
Recent study showed that Biotium CF® Dyes enabled multiplexing of 30-40 fluorescent colors in spectral flow cytometry, which is a new record!
CF® Dye Advantages:
• Available in over 30 colors, offering the greatest coverage across visible and near-IR wavelengths
• Widest selection of spectrally unique dyes to accommodate highly complex panels
• CF® Dye antibody conjugates have superior brightness and signal-to-noise over alternative fluorophores
Save 16%
on all CF® Dye products for flow cytometry
• Mix-n-Stain Antibody labeling kits
• CF® Dye SE Protein labeling kits
• CF® Dye conjugated secondary antibodies
• Live-or-Dye Fixable Viability Stains
• NucView® Caspase-3 reagents
• ViaFluor® SE Cell Proliferation Kits
Flow-validated antibodies
Can you trust your antibody?
When choosing Cell Signaling Technology’s flow-validated antibodies you can be sure that the signal comes from the correct target.
Cross-platform validation is used to confirm the antibody specificity. In addition, all antibodies are been tested for optimal dilution, specificity, stability and lot-to-lot reproducibility to ensure they work the first time, every time.
Talk to Juho!
Juho Niva
Tel. 020 7410 276
Do you need Carrier-free antibodies for CyTOF or other
techniques?
We can help you!
Just contact Juho for more information.
-10%
on all
FACS-validated
antibodies
SoluLINK streptavidin magnetic and agarose beads have the highest biotin binding capacities in the market. The key to high biotin binding is in the unique covalently crosslinked streptavidin, based on SoluLINK’s proprietary linking technology.
NanoLink Streptavidin Magnetic Beads
• Highest biotin binding capacity of any magnetic bead on the market
• Small, approximately 1 µm diameter (high surface area to volume ratio)
• Best for ultra-high capacity immobilizations
• Polydisperse size distribution MagnaLink Streptavidin Magnetic Beads
• Highest biotin binding capacity of any magnetic bead in its size class
• Larger bead, 2.8 µm diameter
• Best for diagnostic applications
• Very uniform size distribution
Streptavidin Agarose Ultra Performance:
• Highest biotin binding capacity of
any agarose bead
• 6% crosslinked agarose and uniform 35 µm structure enable high flow rates
• Best for large-scale immobilizations and purifications
Valid until 31.03.2021 with promo code BN-WINTER21 11
CELLEDYRKNING
Let's talk
...about bioconjugation
Conjugate a biomolecule using SoluLINK!
SoluLINK bioconjugation technology mildly, efficiently, and reproducibly conjugates and immobilizes all categories of biomolecules, including proteins, peptides, oligo-nucleotides, carbohydrates, drugs, and surfaces.
• Easy to use — regardless of your level of experience with conjugation, our detailed step-by-step protocols clearly explain each part of the modification and conjugation process
• Quantifiable — SoluLINK linkers are chromogenic, so you can quantify the molar substitution ratios of HyNic and 4FB on your biomolecules prior to conjugation, as well as the degree of conjugation in real-time during the linking process; a simple UV scan ensures reproducibility of your reactions
• Rapid conjugation — each kit contains TurboLINK™ catalyst to accelerate the
conjugation of HyNic- and 4FB-modified biomolecules at least 10-fold
Talk to Juho!
Juho Niva [email protected]
Tel. 020 7410 276
Capture biomolecules with SoluLINK magnetic
and agarose beads
Save 12% on all
SoluLINK bioconjugation kits, HyNic-linkers,
4FB-linkers, magnetic and agarose beads
BioNordika Oy (Finland) • Kutomotie 18, 00380 Helsinki
tel 0207 410 270 • [email protected] • www.bionordika.fi
Promo code: BN-WINTER21
Note! The promotional code must be referenced when ordering to receive the discounts and special prices. The offers are listed ex. VAT and shipping charges, and are valid until 31.03.2021. The offers cannot be combined with other offers or agreements. We reserve the right for typos.
Now more than ever, QC professionals are looking for solutions to optimize endotoxin testing laboratory operations. Learn how to modernize the bacterial endotoxins test through error-proofing and future-proofing methods.
• Recombinant Factor C (rFC) equivalency to the LAL test
• Efficiencies gained in workstreams by implementation of a robotic system
• PyroCellTM Monocyte Activation Test (MAT) for in vitro testing of endotoxins and non-endotoxin pyrogens Log in to the Lonza’s QC Insider™ Toolbox to access a lot of useful material!
May we introduce Dr. Limulus?
Virtual exhibition booth with NEB 1.-5.2.2021:
Have a chat with us and try to find a little heart hiding in the booth
and you will get a nice surprise. The virtual booth will open on Monday 1.2.2021. Welcome!
HTG webinar:
HTG Molecular is specialised in molecular profiling. Welcome to hear more about HTG
EdgeSeq miRNA Whole Transcriptome Assay and applications.
Intensive
ChIP workshop
by Diagenode in February. Choose from two date
9-10.2
. or
16-17.2
. The work
-shop includes a mix of lecture, demo and activities to help you master the basics and more of ChIP-qPCR and
ChIP-seq.
Enrollment and more information from [email protected]
we cannot meet,
but we are virtually
present!
Stay tuned for
upcoming