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Blockade of the Hedgehog pathway downregulates estrogen receptor alpha signaling in breast cancer cells

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Figure

Figure 1: Characterization of tamoxifen sensitive MCF7 and tamoxifen resistant LCC2 breast cancer cells
Figure 2: Depletion of GLI1 or ERα reduces the proliferation of MCF7 and LCC2 cells. (control siRNA (siCN), GLI1 siRNA (siGLI1) or ERα siRNA (siERα) for 48 hours, was determined by Western blot
Figure 3: GLI1 depletion reduces the activity of an ERα reporter. MCF7 and LCC2 cells were transfected with control siRNA (siControl) or GLI1 siRNA (siGLI1) and after 24 hours were co-transfected with the reporter plasmid ERE-TK-Luc and the pRL-TK control
Table 1: Primer sequences for qRT-PCR analysis
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