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ERN1 and ALPK1 inhibit differentiation of bi-potential tumor- initiating cells in human breast cancer

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Figure

Figure 1: Screening for inducers of differentiation. A. Summary of the lentiviral screen on MDA-MB-468 cells
Figure 2: shRNA-mediated knockdown of ERN1 and ALPK1 reduces mRNA and protein expression
Figure 3: siRNA knockdown of ERN1 and ALPK1 enriches for a luminal cell type. A, B. qPCR data of ERN1 mRNA (A) and ALPK1 mRNA (B) expression in MDA-MB-468 cells transfected with siRNA targeting either ERN1 or ALPK1 mRNA or GFP (control) (n=3)
Figure 4: MDA-MB-468 cells with an ERN1 or ALPK1 knockdown form fewer colonies in 2D and 3D and develop fewer and slower growing tumors
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