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Single cell profiling of human gliomas reveals macrophage ontogeny as a basis for regional differences in macrophage activation in the tumor microenvironment

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Figure

Fig. 1 ScRNA-seq of neoplastic and immune cells from human primary gliomas.somatic mutations that are clonal in exome sequencing (derived from glioma biopsies, were subjected to scRNA-seq ( a Both whole-tumor and CD11b-purified single-cell suspensions,top)
Fig. 2 Analysis of published data identifies markers of ontogeny.derived and microglial TAMs in mouse (outer circle); (2) their homologues; (3) genes expressed (mean CPM > 1) in human BMDM/microglia fromnon-malignant tissue; and (4) TAMs from human gliomas
Fig. 3 (See legend on next page.)
Figure S4a), compared to microglial TAMs. Intri-
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