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SIRT1 promotes proliferation and inhibits the senescence-like phenotype in human melanoma cells

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Figure

Figure 1: SIRT1 activity is elevated in melanoma cells. SIRT1 activity was determined in melanoma cells of different genetic background and in normal melanocytes using an in vitro deacetylation assay
Figure 2: SIRT1 suppression triggers growth arrest. (A-B) Western blot analysis of 501mel cells transfected with a control siRNA (siC) or two SIRT1 specific siRNAs (siSIRT1 and siSIRT1#2) for 96 hrs
Figure 4: MITF regulates the activity of SIRT1. (A) In vitro deacetylation assay in cells transfected with control, MITF or two different SIRT1 siRNA
Figure 5: MITF regulates SIRT1 expression at the transcriptional level. (A) qRT-PCR analysis of the genes indicated on the figure in 501mel melanoma cells transfected with control, MITF or SIRT1 siRNA for 96 hrs
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