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Structural basis for the indispensable role of a unique zinc finger motif in LNX2 ubiquitination

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Figure

Figure 1: Identification of E2 for FL-LNX2 using E2 profiling and its autoubiquitination activity
Figure 2: Polyubiquitination of Human Numb by LNX2. In vitro ubiquitination assays were performed for Numb in the presence (+) or absence (−) of each of E1, E2 (UbcH5b), FL-LNX2, GST-Numb, GST, Ubiquitin (Ub) and ATP respectively
Figure 3: Effect of autoubiquitination of LNX2 on Numb ubiquitination. In vitro ubiquitination assays were performed using E1, E2 (UbcH5b), unmodified FL-LNX2 or MBP beads immobilized autoubiquitinated FL-LNX2, GST-Numb, Ubiquitin and ATP at different time
Figure 4: Autoubiquitination of Zn-RING-Zn domain of LNX2. In vitro ubiquitination assays were performed for the Zn-RING-Zn domain in the presence (+) or absence (−) of each of E1, E2 (UbcH5b), Zn-RING-Zn domain, Ubiquitin and ATP, respectively
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