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β2-microglobulin induces epithelial-mesenchymal transition in human renal proximal tubule epithelial cells in vitro

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Figure

Figure 1 Effects of β2-M treatment on the viability of HK-2 cells.HK-2 cells were grown to 80% confluence, and then, differentconcentrations of β2-M (5, 10, 25, and 50 μM) were added to themedia for an additional 24, 48, and 72 h
Figure 3 Effect ofto β2-M treatment on EMT in HK-2 cells. (A) HK-2 cells were stimulated with 25 μM β2-M for 72 h, Cell lysates were immunoblottedwith antibodies against E-cadherin, α-SMA, fibronectin, vimentin, and β-actin
Figure 5 The β2-M/HFE complex modulated iron levels and induced HIF-1α expression. (A) Iron levels in HK-2 cells and HFE knockdown cellswere measured by inductively coupled plasma mass spectroscopy
Figure 6 Effect of iron on EMT in HK-2 cells. (A) HK-2 cells were stimulated with DES for 48 h, and photographs were taken using a Nikon TMSmicroscope at 400x magnification

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